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Ultrastructure Techniques for Microorganisms

Ultrastructure Techniques for Microorganisms PDF Author: H.C. Aldrich
Publisher: Springer Science & Business Media
ISBN: 1468451197
Category : Medical
Languages : en
Pages : 540

Book Description
The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.

Ultrastructure Techniques for Microorganisms

Ultrastructure Techniques for Microorganisms PDF Author: H.C. Aldrich
Publisher: Springer Science & Business Media
ISBN: 1468451197
Category : Medical
Languages : en
Pages : 540

Book Description
The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.

Microbial Ultrastructure

Microbial Ultrastructure PDF Author: Society for Applied Bacteriology
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 364

Book Description


Electron Microscopy in Microbiology

Electron Microscopy in Microbiology PDF Author:
Publisher: Academic Press
ISBN: 0080860494
Category : Science
Languages : en
Pages : 443

Book Description
This volume of this acclaimed series deals with electron microscopic techniques applied for the elucidation of microbial structures and structure-function relationships at cellular, sub-cellular, and macromolecular levels. Many of the recent findings on ultrastructural features of microorganisms have been obtained with newly developed methods, though classical approaches have not lost their validity. Therefore, both conventional and new methods have been incorporated into this volume. The topics dealt with are meaningful not only in bacterial cytology but also in physiology, enzymology, biochemistry, and molecular biology, and include aspects of medical and biotechnological application.

Fixation Techniques for Two Newly Discovered Bacteria Used to Determine Their Ultrastructure in the Transmission Electron Microscope

Fixation Techniques for Two Newly Discovered Bacteria Used to Determine Their Ultrastructure in the Transmission Electron Microscope PDF Author: Peter Feiner
Publisher:
ISBN:
Category :
Languages : en
Pages : 76

Book Description


Methods for General and Molecular Microbiology

Methods for General and Molecular Microbiology PDF Author: C. A. Reddy
Publisher: American Society for Microbiology Press
ISBN: 1555812236
Category : Science
Languages : en
Pages : 2949

Book Description
A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

Bacterial Cell Wall

Bacterial Cell Wall PDF Author: J.-M. Ghuysen
Publisher: Elsevier
ISBN: 0080860877
Category : Science
Languages : en
Pages : 607

Book Description
Studies of the bacterial cell wall emerged as a new field of research in the early 1950s, and has flourished in a multitude of directions. This excellent book provides an integrated collection of contributions forming a fundamental reference for researchers and of general use to teachers, advanced students in the life sciences, and all scientists in bacterial cell wall research. Chapters include topics such as: Peptidoglycan, an essential constituent of bacterial endospores; Teichoic and teichuronic acids, lipoteichoic acids, lipoglycans, neural complex polysaccharides and several specialized proteins are frequently unique wall-associated components of Gram-positive bacteria; Bacterial cells evolving signal transduction pathways; Underlying mechanisms of bacterial resistance to antibiotics.

Microbiology

Microbiology PDF Author: Holly Ahern
Publisher:
ISBN: 9781942341536
Category : Microbiology
Languages : en
Pages : 140

Book Description
As a group of organisms that are too small to see and best known for being agents of disease and death, microbes are not always appreciated for the numerous supportive and positive contributions they make to the living world. Designed to support a course in microbiology, Microbiology: A Laboratory Experience permits a glimpse into both the good and the bad in the microscopic world. The laboratory experiences are designed to engage and support student interest in microbiology as a topic, field of study, and career. This text provides a series of laboratory exercises compatible with a one-semester undergraduate microbiology or bacteriology course with a three- or four-hour lab period that meets once or twice a week. The design of the lab manual conforms to the American Society for Microbiology curriculum guidelines and takes a ground-up approach -- beginning with an introduction to biosafety and containment practices and how to work with biological hazards. From there the course moves to basic but essential microscopy skills, aseptic technique and culture methods, and builds to include more advanced lab techniques. The exercises incorporate a semester-long investigative laboratory project designed to promote the sense of discovery and encourage student engagement. The curriculum is rigorous but manageable for a single semester and incorporates best practices in biology education.

International Review of Cytology

International Review of Cytology PDF Author:
Publisher: Academic Press
ISBN: 0080569471
Category : Science
Languages : en
Pages : 304

Book Description
International Review of Cytology presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.

Biological Electron Microscopy

Biological Electron Microscopy PDF Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
ISBN: 146840010X
Category : Science
Languages : en
Pages : 368

Book Description
In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

Electron Microscopy Of Subcellular Dynamics

Electron Microscopy Of Subcellular Dynamics PDF Author: Helmut Plattner
Publisher: CRC Press
ISBN: 9780849360794
Category : Science
Languages : en
Pages : 390

Book Description
This illustrated volume surveys the correlated use of currently available methods of electron microscopic techniques, along with the goals and perspectives for future developments. The authors discuss an integrative approach of different EM preparation and analysis techniques that can allow for an analysis of dynamic cellular processes with high temporal and spatial resolution on the electron microscope level. This concise, yet thorough, work is a valuable reference for researchers in the field.