Author: Jan Michiels Publisher: Humana ISBN: 9781493928538 Category : Medical Languages : en Pages : 0
Book Description
This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.
Author: Juan Carlos Alonso Publisher: Mdpi AG ISBN: 9783036506746 Category : Science Languages : en Pages : 170
Book Description
Bacterial toxin-antitoxin (TA) systems, which are ubiquitously present in bacterial genomes, are not essential for normal cell proliferation. The TA systems regulate fundamental cellular processes, facilitate survival under stress conditions, have essential roles in virulence and represent potential therapeutic targets. These genetic TA loci are also shown to be involved in the maintenance of successful multidrug-resistant mobile genetic elements. The TA systems are classified as types I to VI, according to the nature of the antitoxin and to the mode of toxin inhibition. Type II TA systems encode a labile antitoxin and its stable toxin; degradation of the antitoxin renders a free toxin, which is bacteriostatic by nature. A free toxin generates a reversible state with low metabolic activity (quiescence) by affecting important functions of bacterial cells such as transcription, translation, DNA replication, replication and cell-wall synthesis, biofilm formation, phage predation, the regulation of nucleotide pool, etc., whereas antitoxins are toxin inhibitors. Under stress conditions, the TA systems might form networks. To understand the basis of the unique response of TA systems to stress, the prime causes of the emergence of drug-resistant strains, and their contribution to therapy failure and the development of chronic and recurrent infections, must be known in order to grasp how TA systems contribute to the mechanisms of phenotypic heterogeneity and pathogenesis that will enable the rational development of new treatments for infections caused by pathogens.
Author: Kim Lewis Publisher: Springer Nature ISBN: 3030252418 Category : Medical Languages : en Pages : 300
Book Description
This volume is a collection of chapters from the leading experts in the relatively new and burgeoning field of persister cell studies. Persisters play a leading role in the recalcitrance of chronic infections, and enable the development of classical antibiotic resistance. The focus of the book is on studies that provide an understanding of the mechanisms of persister formation, antibiotic tolerance and role in disease, at the molecular level.
Author: P. Gopalakrishnakone Publisher: Springer ISBN: 9789400764484 Category : Medical Languages : en Pages : 502
Book Description
In recent years, the field of Toxinology has expanded substantially. On the one hand it studies venomous animals, plants and micro organisms in detail to understand their mode of action on targets. While on the other, it explores the biochemical composition, genomics and proteomics of toxins and venoms to understand their three interaction with life forms (especially humans), development of antidotes and exploring their pharmacological potential. Therefore, Toxinology has deep linkages with biochemistry, molecular biology, anatomy and pharmacology. In addition, there is a fast developing applied subfield, clinical toxinology, which deals with understanding and managing medical effects of toxins on human body. Given the huge impact of toxin-based deaths globally, and the potential of venom in generation of drugs for so-far incurable diseases (for example, Diabetes, Chronic Pain), the continued research and growth of the field is imminent. This has led to the growth of research in the area and the consequent scholarly output by way of publications in journals and books. Despite this ever growing body of literature within biomedical sciences, there is still no all-inclusive reference work available that collects all of the important biochemical, biomedical and clinical insights relating to Toxinology. The Handbook of Toxinology aims to address this gap and cover the field of Toxinology comprehensively.
Author: P. Gopalakrishnakone Publisher: Springer ISBN: 9789400758681 Category : Medical Languages : en Pages : 0
Book Description
Biological toxins are an important part of our world, a reality with which we need to cope, so in parallel with understanding their mechanisms of action and thereby improving our fundamental knowledge, there are successful efforts to utilize them as therapeutics against some debilitating human and animal diseases. In view of the complexity of different types of biotoxins and the broad range of toxin structure, physiology, utility, and countermeasures including regulatory issues, it was thus aimed to compile a book on biotoxins and bioweapons. This reference work in the Toxinology handbook series gathers together knowledge from around the globe about naturally inspired and manufactured biological weapons. The authors describe how they work; how authorities may detect their presence, prevent their use, and diagnose their impacts; and the means by which medical and paramedical professionals may treat victims. Also described are how they have been used to further our knowledge and what insights they have given us into evolutionary and physiological processes. Finally, it is also discussed how these toxins can be used as therapeutics and what the implications of such therapeutics are to their use as biothreat agents. This volume provides a reference accessible to scientists, educators, and medical experts alike with an interest in biotoxins, focusing on the major toxins used as bioweapons. Regulatory agencies will also benefit from the information provided in this book. Some in the intended audience may need to understand how they elicit their effects and how we can defend ourselves against them. Others may be interested in the sometimes colorful histories that surround this subset of biotoxins that can be and, in some cases, have been used as weapons.
Author: A.L. Burlingame Publisher: Gulf Professional Publishing ISBN: 9780121828073 Category : Science Languages : en Pages : 520
Book Description
Describes and integrates the techniques of many advances in both chromatographic and mass spectrometric technologies. This book also covers various biophysical applications, such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. It also describes atto-to-zepto-mole quantitation of 14C and 3H.
Author: Gregory R. Ciottone Publisher: Elsevier Health Sciences ISBN: 0323286658 Category : Medical Languages : en Pages : 1050
Book Description
The most comprehensive resource of its kind, Ciottone's Disaster Medicine, 2nd Edition, thoroughly covers isolated domestic events as well as global disasters and humanitarian crises. Dr. Gregory Ciottone and more than 200 worldwide authorities share their knowledge and expertise on the preparation, assessment, and management of both natural and man-made disasters, including terrorist attacks and the threat of biological warfare. Part 1 offers an A-to-Z resource for every aspect of disaster medicine and management, while Part 2 features an exhaustive compilation of every conceivable disaster event, organized to facilitate quick reference in a real-time setting. Quickly grasp key concepts, including identification of risks, organizational preparedness, equipment planning, disaster education and training, and more advanced concepts such as disaster risk reduction, tactical EMS, hazard vulnerability analysis, impact of disaster on children, and more. Understand the chemical and biologic weapons known to exist today, as well as how to best manage possible future events and scenarios for which there is no precedent. Be prepared for man-made disasters with new sections that include Topics Unique to Terrorist Events and High-Threat Disaster Response and Operational Medicine (covering tactical and military medicine). Get a concise overview of lessons learned by the responders to recent disasters such as the earthquake in Haiti, Hurricane Sandy, the 2014 Ebola outbreak, and active shooter events like Sandy Hook, CT and Aurora, CO. Learn about the latest technologies such as the use of social media in disaster response and mobile disaster applications. Ensure that everyone on your team is up-to-date with timely topics, thanks to new chapters on disaster nursing, crisis leadership, medical simulation in disaster preparedness, disaster and climate change, and the role of non-governmental agencies (NGOs) in disaster response - a critical topic for those responding to humanitarian needs overseas. Expert Consult eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, and references from the book on a variety of devices.
Author: Luis P. Villarreal Publisher: Springer Science & Business Media ISBN: 0387779981 Category : Science Languages : en Pages : 632
Book Description
A sense of belonging is basic to the human experience. But in this, humans are not unique. Essentially all life, from bacteria to humans, have ways by which it determines which members belong and which do not. This is a basic cooperative nature of life I call group membership which is examined in this book. However, cooperation of living things is not easily accounted for by current theory of evolutionary biology and yet even viruses display group membership. That viruses have this feature would likely seem coincidental or irrelevant to most scientist as having any possible relationship to human group identity. Surely such simple molecular-based relationships between viruses are unrelated to the complex cognitive and emotional nature of human group membership. Yet viruses clearly affect bacterial group membership, which are the most diverse and abundant cellular life form on Earth and from which all life has evolved. Viruses are the most ancient, numerous and adaptable biological entities we know. And we have long recognized them for the harm and disease they can cause, and they have been responsible for the greatest numbers of human deaths. However, with the sequencing of entire genomes and more recently with the shotgun sequencings of habitats, we have come to realize viruses are the black hole of biology; a giant force that has until recently been largely unseen and historically ignored by evolutionary biology. Viruses not only can cause acute disease, but also persist as stable unseen agents in their host.
Author: Seyed Ehtesham Hasnain Publisher: Springer ISBN: 9789813294127 Category : Medical Languages : en Pages : 514
Book Description
This book reviews recent advances in the molecular and infection biology, pathology, and molecular epidemiology of Mycobacterium tuberculosis, as well as the identification and validation of novel molecular drug targets for the treatment of this mycobacterial disease. Despite being completely curable, tuberculosis is still one of the leading global causes of death. M. tuberculosis, the causative organism – one of the smartest pathogens known – adopts highly intelligent strategies for survival and pathogenesis. Presenting a wealth of information on the molecular infection biology of M. tuberculosis, as well as nontuberculous mycobacteria (NTM), the book provides an overview of the functional role of the PE/PPE group of proteins, which is exclusive to the genus Mycobacteria, of host-pathogen interactions, and virulence. It also explores the pathogenesis of the infection, pathology, epidemiology, and diagnosis of NTM. Finally it discusses current and novel approaches in vaccine development against tuberculosis, including the role of nanotechnology. With state-of-the-art contributions from experts in the respective domains, this book is an informative resource for practitioners as well as medical postgraduate students and researchers.
Author: Jan Michiels
Author: Juan Carlos Alonso
Author: Kim Lewis
Author: P. Gopalakrishnakone
Author: P. Gopalakrishnakone
Author: A.L. Burlingame
Author: Gregory R. Ciottone
Author: P. Gopalakrishnakone
Author: Luis P. Villarreal
Author: Seyed Ehtesham Hasnain