Author: Chen, XiPublisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
The DNA-binding Specificity of Forkhead Transcription Factors
Author: Chen, XiA Handbook of Transcription Factors
Author: Timothy R. HughesPublisher: Springer Science & Business Media
ISBN: 904819069X
Category : Medical
Languages : en
Pages : 310
Book Description
Transcription factors are the molecules that the cell uses to interpret the genome: they possess sequence-specific DNA-binding activity, and either directly or indirectly influence the transcription of genes. In aggregate, transcription factors control gene expression and genome organization, and play a pivotal role in many aspects of physiology and evolution. This book provides a reference for major aspects of transcription factor function, encompassing a general catalogue of known transcription factor classes, origins and evolution of specific transcription factor types, methods for studying transcription factor binding sites in vitro, in vivo, and in silico, and mechanisms of interaction with chromatin and RNA polymerase.
DNA Binding Specificity of Human Transcription Factors Oct-1 and USF as Revealed by Random DNA Sequence Selection Strategies
Author: Andrew John BendallPublisher:
ISBN:
Category : Binding sites (Biochemistry)
Languages : en
Pages : 288
Book Description
Exploring the DNA-binding Specificities of Mouse Transcription Factors
Author: Shaheynoor TalukderPublisher:
ISBN: 9780494403037
Category :
Languages : en
Pages : 222
Book Description
Two striking findings of vertebrate genome sequencing are the presence of large numbers of DNA binding proteins and the high proportion of conserved non-coding sequences, much of which is cis-regulatory. Lack of comprehensive knowledge about binding specificities of transcription factors (TFs) complicates de novo computational searches for novel cis-regulatory elements. To address the paucity of information on binding affinity of TFs, I aimed to characterize the DNA-binding specificities of & sim;1500 murine TFs. I was involved in the construction of a library of DNA-binding domains (DBDs) of mouse TFs. I utilized this library to express/purify the DBDs for application on Protein Binding Microarrays, an array-based approach used to determine binding specificity of TFs. Analysis of preliminary PBM results are consistent with known binding specificities for TFs. Data for uncharacterized TFs yielded diverse new motifs. The data will provide a valuable resource in understanding the relationship between TFs and their cis-regulatory elements.
Visualizing the Full Landscape of DNA Binding Specificity for the Development of Artificial Transcription Factors
Author: Clayton D. CarlsonTranscription Factors
Author: David LatchmanPublisher: OUP Oxford
ISBN: 0191565792
Category : Science
Languages : en
Pages : 326
Book Description
Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library. Chapter 6 deals with identification of transcription factors based on sequence homology analysis by both experimental screening and database searches. Chapter 7 is a new chapter that describes methods of identifying the target genes of a previously uncharacterized factor. The next chapters deal with analysis of transcription factor function. Chapter 8 deals with general techniques, and then the following chapters cover the specialized techniques of in vitro transcription assays using transcriptionally active nuclear extracts derived from rat brain, and analysis of the effect of transcription factors on chromatin structure. The final chapter describes methods for detecting the phosphorylation and glycosylation state of transcription factors.
Forkhead FOXO Transcription Factors in Development and Disease
Author: Publisher: Academic Press
ISBN: 0128043369
Category : Science
Languages : en
Pages : 226
Book Description
Forkhead FOXO Transcription Factors in Development and Disease, Volume 127, the latest release in the Current Topics in Developmental Biology series, provides a comprehensive set of reviews on transcription factors FOXO and their various functions in health and disease. Each chapter is contributed by a leading expert in the field, with specific sections covering Exceptional Longevity: Insights from Hydra to Humans, FOXO in neural cells and diseases of the nervous system, maintaining the equilibrium for better or for worse, and The Role of FoxOs in Bone Health and Disease, amongst other topics. This compilation provides a unique, up-to-date view of this enigmatic, highly evolutionary conserved family of transcription factors with a diverse array of functions in aging and longevity, cancer, and metabolism. Provides the authority and expertise of leading contributors Includes sections on the regulation of FOXO factors and stem cells Presents a unique view of this highly evolutionary family of transcription factors
Gene Regulation in Eukaryotes
Author: Edgar WingenderPublisher: Wiley-Blackwell
ISBN:
Category : Science
Languages : en
Pages : 452
Book Description
A much-needed guide through the overwhelming amount of literature in the field. Comprehensive and detailed, this book combines background information with the most recentinsights. It introduces current concepts, emphasizing the transcriptional control of genetic information. Moreover, it links data on the structure of regulatory proteins with basic cellular processes. Both advanced students and experts will find answers to such intriguing questions as: - How are programs of specific gene repertoires activated and controlled? - Which genes drive and control morphogenesis? - Which genes govern tissue-specific tasks? - How do hormones control gene expression in coordinating the activities of different tissues? An abundant number of clearly presented glossary terms facilitates understanding of the biological background. Speacial feature: over 2200 (!) literature references.
DNA Binding Specificity and Transcriptional Regulation of Six4
Author: Theodore KiossesPublisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Attaining an understanding of the mechanisms underpinning development has been amongst the cardinal scientific challenges of our age. The transition from a single cell organism to the level of complexity evidenced in higher eukaryotes has been facilitated by the advent of intricate developmental networks involving a plethora of factors that synergise to allow for precise spatio-temporal expression of the proteins present in higher organisms. Development is often portrayed as a domino like cascade of events stemming from relatively uncomplicated origins that go on to branch out and form associations and interactions amongst multitudinous actors that will inexorably lead towards a higher state of order. Transcription factors occupy a central position within this tapestry of interactions. They regulate expression of the various required proteins and they provide the cues for the developmental events that will eventually shape an organism. These factors frequently remain unknown until some occurrence causes developmental processes to fail and inadvertently focus attention on the factors that facilitate development. Myotonic dystrophy is a useful paradigm of such a developmental dysfunction that has led to the discovery of a transcription factor integral to both muscle development and gonadogenesis in both Drosophila and higher eukaryotes.
Author: Ka Chun Wong