Single Molecule Microscopy in Neurobiology PDF Download

Author: Nobuhiko Yamamoto
Publisher: Humana
ISBN: 9781071605349
Category : Medical
Languages : en
Pages : 331

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Book Description
This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.

Author: Fritjof Helmchen
Publisher:
ISBN: 9780879699376
Category : Medical
Languages : en
Pages : 0

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Book Description
As imaging technologies have revolutionized research in many areas of biology and medicine, neuroscientists have often pioneered the use of these new visualization techniques. This volume is an essential guide to discovering and implementing these techniques in the neuroscience lab.

Author: Rafael Yuste
Publisher: CSHL Press
ISBN: 0879696923
Category : Medical
Languages : en
Pages : 813

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Book Description
As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.

Author: Rusty Lansford
Publisher:
ISBN: 9781621820130
Category : Genes
Languages : en
Pages : 0

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Book Description
A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.

Author: Matt Carter
Publisher: Academic Press
ISBN: 0323915612
Category : Medical
Languages : en
Pages : 416

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Book Description
Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step

Author: Haruyuki Kamiya
Publisher: Frontiers Media SA
ISBN: 2889661873
Category : Science
Languages : en
Pages : 272

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Book Description
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Author: C. U. M. Smith
Publisher: John Wiley & Sons
ISBN: 047085717X
Category : Medical
Languages : en
Pages : 630

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Book Description
This edition of the popular text incorporates recent advances in neurobiology enabled by modern molecular biology techniques. Understanding how the brain works from a molecular level allows research to better understand behaviours, cognition, and neuropathologies. Since the appearance six years ago of the second edition, much more has been learned about the molecular biology of development and its relations with early evolution. This "evodevo" (as it has come to be known) framework also has a great deal of bearing on our understanding of neuropathologies as dysfunction of early onset genes can cause neurodegeneration in later life. Advances in our understanding of the genomes and proteomes of a number of organisms also greatly influence our understanding of neurobiology. * Well known and widely used as a text throughout the UK, good reviews from students and lecturers. * Good complement to Fundementals of Psychopharmacology by Brian Leonard. This book will be of particular interest to biomedical undergraduates undertaking a neuroscience unit, neuroscience postgraduates, physiologists, pharmacologists. It is also a useful basic reference for university libraries. Maurice Elphick, Queen Mary, University of London "I do like this book and it is the recommended textbook for my course in Molecular Neuroscience. The major strength of the book is the overall simplicity of the format both in terms of layout and diagrams."

Author: Robert D. Goldman
Publisher: CSHL Press
ISBN: 9780879696832
Category : Science
Languages : en
Pages : 658

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Book Description
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

Author: Peter J. Verveer
Publisher: Humana
ISBN: 9781493942527
Category : Medical
Languages : en
Pages : 0

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Book Description
This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Author: Krishnarao Appasani
Publisher: Cambridge University Press
ISBN: 1108423361
Category : Medical
Languages : en
Pages : 171

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Book Description
A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.