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Author: Ke-li Han Publisher: Springer Science & Business Media ISBN: 3319029703 Category : Medical Languages : en Pages : 488
Book Description
This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression. On the experimental side, the technical advances have offered deep insights into the conformational motions of a number of proteins. These studies greatly enrich our knowledge of the interplay between structure and function. On the theoretical side, novel approaches and detailed computational simulations have provided powerful tools in the study of enzyme catalysis, protein / drug design, protein / ion / other molecule translocation and protein folding/aggregation, to name but a few. This work contains detailed information, not only on the conformational motions of biological systems, but also on the potential governing forces of conformational dynamics (transient interactions, chemical and physical origins, thermodynamic properties). New developments in computational simulations will greatly enhance our understanding of how these molecules function in various biological events.
Author: Ke-li Han Publisher: Springer Science & Business Media ISBN: 3319029703 Category : Medical Languages : en Pages : 488
Book Description
This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression. On the experimental side, the technical advances have offered deep insights into the conformational motions of a number of proteins. These studies greatly enrich our knowledge of the interplay between structure and function. On the theoretical side, novel approaches and detailed computational simulations have provided powerful tools in the study of enzyme catalysis, protein / drug design, protein / ion / other molecule translocation and protein folding/aggregation, to name but a few. This work contains detailed information, not only on the conformational motions of biological systems, but also on the potential governing forces of conformational dynamics (transient interactions, chemical and physical origins, thermodynamic properties). New developments in computational simulations will greatly enhance our understanding of how these molecules function in various biological events.
Author: Dennis R. Livesay Publisher: Humana ISBN: 9781493963072 Category : Science Languages : en Pages : 0
Book Description
In Protein Dynamics: Methods and Protocols, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting modern protein research. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Dynamics: Methods and Protocols describes the most common and powerful methods used to characterize protein dynamics.
Author: Rory Sleno Publisher: ISBN: Category : Languages : en Pages :
Book Description
"As key effector units of the cell, understanding structure and function relationships of proteins are integral to explaining physiology and, in turn, pathophysiology. With the cost of drug development soaring and success dwindling, new ways to probe protein function will be at the forefront of innovation. A protein's function is the product of its conformational dynamics, where distinct 3-dimensional conformations of a protein dictate its various effects on the cell. Though highly valuable technologies capable of reporting on protein conformation currently exist, X-ray crystallography and nuclear magnetic resonance among others, their capacity to report on dynamic events and/or in physiologically relevant assay conditions are limited.This thesis describes the development and application of biosensors capable of reporting on protein conformation in live human cells. Biosensors were developed for two classes of protein targets, ion channels - Kir3, and G protein-coupled receptors (GPCR) - FP and AT1R, together representing the targets of a majority of current prescription drugs. The biosensor design was based on bioluminescence resonance energy transfer (BRET) and required the insertion of a bioluminescent protein and a short peptide sequences within the protein of interest. Due to the specific affinity for the peptide sequence of an organic fluorophore, fluorescein biarsenical hairpin binder (FlAsH), the latter could be used as a resonance energy transfer acceptor to probe the different conformations of the protein of interest without disrupting its function.Both panels of biosensors successfully reported on ligand-induced conformational rearrangements in the target proteins and correlated with functional effects. The GPCR biosensors were further applied to enrich our understanding of receptor dimerization and allosteric communication between proteins, in this case, the FP/AT1R heterodimer." --
Author: Timir Tripathi Publisher: Academic Press ISBN: 0323902650 Category : Science Languages : en Pages : 716
Book Description
Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. - Presents a thorough overview of the latest and emerging methods and technologies for protein study - Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy - Includes computational and machine learning methods - Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins
Author: Ke-li Han
Author: Ke-li Han
Author: Ferruccio Palazzesi
Author: Zhaoning Cui
Author: Jeffrey Alan Hanson
Author: Dennis R. Livesay
Author: Rahul Banerjee
Author: Robert M. Culik
Author: Robel Birru Yirdaw
Author: Rory Sleno
Author: Timir Tripathi