Author: Eric Fung Publisher: Springer Science & Business Media ISBN: 1592597599 Category : Science Languages : en Pages : 279
Book Description
Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies.
Author: Joanna S. Albala Publisher: CRC Press ISBN: 0824756347 Category : Science Languages : en Pages : 422
Book Description
From disease marker identification to accelerated drug development, Protein Arrays, Biochips, and Proteomics offers a detailed overview of current and emerging trends in the field of array-based proteomics. This reference focuses on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein-protein interactions, structural proteomics, and the proteomic marketplace for comprehensive understanding of past, present, and future proteomic research. Offering an abundance of figures and charts, the book compiles a wide variety of technologies and applications ranging from functionalized chip surfaces to strategies for protein expression.
Author: Rodrigo Barderas Publisher: Humana ISBN: 9781071615614 Category : Science Languages : en Pages : 282
Book Description
This volume focuses on protein analysis, and covers a wide array of uses of protein microarray for disease analysis. The chapters in this book discuss different stages of protein microarrays from their construction to their use, including different types of protein microarrays such as recombinant proteins, antibody, phage, and NAPPA protein microarrays, in planar format or in solution via beads arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Protein Microarrays for Disease Analysis: Methods and Protocols is a valuable resource for graduate and post-doctoral fellows interested in protein microarrays, as well as senior researchers interested in gaining more insight into this developing field.
Author: Dev Kambhampati Publisher: Wiley-Blackwell ISBN: Category : Medical Languages : en Pages : 284
Book Description
Protein microarrays soon will become one of the major tools for realizing the goals of drug discovery research in the post-genome era. The book is aimed at life science researchers and scientists working in drug and pharma industry.
Author: Mark Schena Publisher: Jones & Bartlett Learning ISBN: 9780763731274 Category : Science Languages : en Pages : 510
Book Description
Protein Microarrays is one of the first books in this exciting new technology and will be an essential reference for most biologists and biotechnology professionals.
Author: Dhammika Amaratunga Publisher: John Wiley & Sons ISBN: 0470317965 Category : Mathematics Languages : en Pages : 270
Book Description
A cutting-edge guide to the analysis of DNA microarray data Genomics is one of the major scientific revolutions of this century, and the use of microarrays to rapidly analyze numerous DNA samples has enabled scientists to make sense of mountains of genomic data through statistical analysis. Today, microarrays are being used in biomedical research to study such vital areas as a drug’s therapeutic value–or toxicity–and cancer-spreading patterns of gene activity. Exploration and Analysis of DNA Microarray and Protein Array Data answers the need for a comprehensive, cutting-edge overview of this important and emerging field. The authors, seasoned researchers with extensive experience in both industry and academia, effectively outline all phases of this revolutionary analytical technique, from the preprocessing to the analysis stage. Highlights of the text include: A review of basic molecular biology, followed by an introduction to microarrays and their preparation Chapters on processing scanned images and preprocessing microarray data Methods for identifying differentially expressed genes in comparative microarray experiments Discussions of gene and sample clustering and class prediction Extension of analysis methods to protein array data Numerous exercises for self-study as well as data sets and a useful collection of computational tools on the authors’ Web site make this important text a valuable resource for both students and professionals in the field.
Author: Paul F. Predki Publisher: CRC Press ISBN: 1420009842 Category : Medical Languages : en Pages : 482
Book Description
An ideal text for biotechnologists, protein chemists, and biochemists, Functional Protein Microarrays in Drug Discovery explores all aspects of functional protein microarrays, including basic principles, methods, and applications. The book discusses the generation of functional protein content and describes both standard and state-of-the-art fabrication methods. It reviews current and next generation approaches to assay detection and presents a wide range of applications, from biomolecular interaction discovery and characterization to immune response profiling. The book also addresses several fundamental computational issues, bioinformatics and data analysis.
Author: Dev Kambhampati Publisher: John Wiley & Sons ISBN: 3527605215 Category : Science Languages : en Pages : 276
Book Description
This book is the first of its kind in the field of protein microarrays and addresses novel strategies for constructing highly functional and biocompatible microarrays for screening proteins. The list of authors consisting of world leading experts provide a roadmap for solving the complex challenges that are currently faced while monitoring protein-protein interactions over a wide range of microarray platforms. In doing so, they also offer a comprehensive overview of microarray surface chemistry, detection technologies, fabrication options for array development, and data analysis of numerous types of protein interactions. Topics covered include: -Types of biomolecular interactions -Surface chemistry -Detection technologies -Spotting technologies -Bioinformatics/data analysis. While primarily intended to serve as a reference for researchers and students embarking on the exciting fields of proteomics, drug discovery and clinical diagnostics, this technology is also expected to potentially impact the areas of food diagnostics, environmental monitoring and national security.
Author: Greta Pifat-Mrzljak Publisher: Springer Science & Business Media ISBN: 1461513634 Category : Science Languages : en Pages : 238
Book Description
This volume represents a collection of lectures delivered by outstanding specialists in the fields of biophysics and of related scientific disciplines th during the 7 International Summer School on Biophysics held in Rovinj, Croatia from 14 to 25 September 2000 under the title "Super molecular Structure and Function ". This scientific-educational event was organized by the Ruder Boskovic Institute ofZagreb, Croatia with substantial material and intellectual support of a number of national and international institutions including the Croatian Biophysical Society (CBS), the International Union of Pure and Applied Biophysics (IUPAB), the International Centre for Genetic Engineering and Biotechnology (ICGEB) and the UNESCO Venice Office - Regional Office for Science and Technology for Europe (UVO-ROSTE). The seventh edition of the series of International Summer Schools on Biophysics, which was started in 198I, attracted more than 120 young researchers and post-graduate students coming from 27 countries of Europe, Asia, Africa and Latin America. Twenty-five outstanding experts in pure and applied biophysics presented the most advanced knowledge ofthis very interdisciplinary area of science during their lectures and round tables. It was commonly acknowledge that the Summer School achieved great success and fully reached its objectives. The success of the Rovinj Summer School was also due to the constantly growing attention being paid by scientific communities to younger generations of scientists, thanks also to the major outcomes of the World Conference on Science "Science for the Twenty-first Century: A New Commitment" held by UNESCO and ICSU in Budapest, Hungary in June 1999.
Author: Uwe R. Müller Publisher: Springer Science & Business Media ISBN: 9783540229315 Category : Medical Languages : en Pages : 414
Book Description
The genomics revolution would not have been possible without the 'parallelisation' offered by microarray technology. This technological - and commercial - success has been since emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved.
Author: Eric Fung
Author: Joanna S. Albala
Author: Rodrigo Barderas
Author: Dev Kambhampati
Author: Mark Schena
Author: Dhammika Amaratunga
Author: Paul F. Predki
Author: Dev Kambhampati
Author: Greta Pifat-Mrzljak
Author: Uwe R. Müller