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Author: Gregory R. Bock Publisher: John Wiley & Sons ISBN: 0470862629 Category : Science Languages : en Pages : 310
Book Description
A comprehensive review of recent work on chromatin and non-histone proteins, this book arises from the interactions of a multidisciplinary group of scientists involved in the study of acetylation. This area of research opens up new and exciting possibilities for drug design, and so the final chapters in the book examine some of the potential applications in the treatment of various diseases.
Author: Gregory R. Bock Publisher: John Wiley & Sons ISBN: 0470862629 Category : Science Languages : en Pages : 310
Book Description
A comprehensive review of recent work on chromatin and non-histone proteins, this book arises from the interactions of a multidisciplinary group of scientists involved in the study of acetylation. This area of research opens up new and exciting possibilities for drug design, and so the final chapters in the book examine some of the potential applications in the treatment of various diseases.
Author: Robert M. Brosh Publisher: ISBN: 9781493994342 Category : Acetylation Languages : en Pages : 310
Book Description
The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.
Author: T. Shantha Raju Publisher: John Wiley & Sons ISBN: 1119053366 Category : Science Languages : en Pages : 293
Book Description
A Comprehensive Guide to Crucial Attributes of Therapeutic Proteins in Biological Pharmaceuticals With this book, Dr. Raju offers a valuable resource for professionals involved in research and development of biopharmaceutical and biosimilar drugs. This is a highly relevant work, as medical practitioners have increasingly turned to biopharmaceutical medicines in their search for safe and reliable treatments for complex diseases, while pharmaceutical researchers seek to expand the availability of biopharmaceuticals and create more affordable biosimilar alternatives. Readers receive a thorough overview of the major co-translational modifications (CTMs) and post-translational modifications (PTMs) of therapeutic proteins relevant to the development of biotherapeutics. The majority of chapters detail individual CTMs and PTMs that may affect the physicochemical, biochemical, biological, pharmacokinetic, immunological, toxicological etc. properties of proteins. In addition, readers are guided on the methodology necessary to analyze and characterize these modifications. Thus, readers gain not only an understanding of CTMs/PTMs, but also the ability to design and assess their own structure-function studies for experimental molecules. Specific features and topics include: Discussion of the research behind and expansion of biopharmaceuticals Twenty chapters detailing relevant CTMs and PTMs of proteins, such as glycosylation, oxidation, phosphorylation, methylation, proteolysis, etc. Each chapter offers an introduction and guide to the mechanisms and biological significance of an individual CTM or PTM, including practical guidance for experiment design and analysis An appendix of biologic pharmaceuticals currently on the market, along with an assessment of their PTMs and overall safety and efficacy This volume will prove a key reference on the shelves of industry and academic researchers involved in the study and development of biochemistry, molecular biology, biopharmaceuticals and proteins in medicine, particularly as biopharmaceuticals and biosimilars become ever more prominent tools in the field of healthcare.
Author: Tanveer Ali Dar Publisher: Academic Press ISBN: 9780128119136 Category : Science Languages : en Pages : 0
Book Description
Protein Modificomics: From Modifications to Clinical Perspectives comprehensively deals with all of the most recent aspects of post-translational modification (PTM) of proteins, including discussions on diseases involving PTMs, such as Alzheimer's, Huntington's, X-linked spinal muscular atrophy-2, aneurysmal bone cyst, angelman syndrome and OFC10. The book also discusses the role PTMs play in plant physiology and the production of medicinally important primary and secondary metabolites. The understanding of PTMs in plants helps us enhance the production of these metabolites without greatly altering the genome, providing robust eukaryotic systems for the production and isolation of desired products without considerable downstream and isolation processes.
Author: William P. Jencks Publisher: Courier Corporation ISBN: 9780486654607 Category : Science Languages : en Pages : 866
Book Description
Exceptionally clear coverage of mechanisms for catalysis, forces in aqueous solution, carbonyl- and acyl-group reactions, practical kinetics, more.
Author: Alexis Jo Lawton (Ph.D.) Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
Reversible protein acetylation is a widespread, regulatory modification that was first discovered on histone proteins, but since has been described throughout the cell in major subcellular compartments. Several functional roles of lysine acetylation have been described including regulating enzymatic activity, protein-protein interactions, protein-DNA interactions, protein stability, and subcellular localization. With the improvements in mass spectrometry technologies, the number of identified acetylated sites has grown exponentially creating a new challenge to determine which sites are functional versus spurious. Furthermore, studies investigating the mechanisms regulating protein acetylation have primarily focused on histone acetylation, but how they may regulate non-histone protein acetylation has not been fully understood. Of importance, one of the main mechanisms through which acetylation may be regulated is through changes in the availability of acetyl-CoA. Since most acetyltransferases, including p300/CBP, have a Km near the estimated cellular concentrations of acetyl-CoA, manipulating concentrations of the substrate through activation of its production can have major effects on the levels of acetylation. Several studies have investigated the effect of knockdowns of acetyl-CoA generating enzymes (such as ACLY or ACSS2), however, few studies have measured the effects on non-histone protein acetylation. My research uses two main approaches to respond to these challenges in the field: 1) a robust, mass spectrometry method to quantify acetylation stoichiometry across the proteome, and 2) investigating dynamics of acetylation in response to changes in acetyl-CoA availability. Understanding both stoichiometry and dynamic responses during cellular stimulation are key features of interrogating the role of protein acetylation at a site- and protein-specific level. My research aims to understand the cellular mechanisms that are controlling dynamic acetylation in response to metabolic changes in acetyl Coenzyme A (acetyl-CoA) availability and production.Here, I examine the dynamics of global protein acetylation and the mechanisms regulating growth-factor stimulated dynamics, with a particular focus on how metabolism and the production and availability of acetyl-CoA is a major regulator of protein acetylation. Chapter 1 introduces protein acetylation and discusses the field's current understanding of acetylation functions, the quantitative techniques for investigating global non-histone protein acetylation, and the mechanisms involved in regulating protein acetylation. Chapter 2 presents an updated method for quantifying lysine acetylation stoichiometry and applies this method to identify serum-stimulated protein acetylation dynamics. Chapter 3 presents an in-progress project that examines the key regulatory nodes controlling growth-factor stimulated acetylation, including the kinase AKT, acetyl-CoA production through ACLY, and citrate export by SLC25A1. Chapter 4 presents the ongoing experiments and future directions for these projects. My thesis work has also allowed me to be a part of some critical collaborations that have investigated the connection between metabolism, acetyl-CoA availability, and protein acetylation in several interesting mouse models. My contributions and our key findings are presented in the Appendixes of this thesis. Appendix 1 investigates the effect of the dysregulation of the ER-localized acetyl-CoA transporter, AT-1, on protein acetylation. Appendix 2 investigates the effect of increased flux of citrate into the cytoplasm and nucleus through overexpression models of the extracellular membrane citrate transporter, SLC13A5, and the mitochondrial citrate transporter, SLC25A1, and their effect on protein acetylation. Appendix 3 investigates the effect of age, diet, and the loss of the mitochondrial localized deacetylase, SIRT3, on mitochondrial protein acetylation. Lastly, Appendix 4 examines the effects of the loss of individual ER-localized acetyltransferases, ATase1 and ATase2, on secretory pathway protein acetylation.
Author: Trygve Tollefsbol Publisher: Academic Press ISBN: 0128032405 Category : Science Languages : en Pages : 944
Book Description
Medical Epigenetics provides a comprehensive analysis of the importance of epigenetics to health management. The purpose of this book is to fill a current need for a comprehensive volume on the medical aspects of epigenetics with a focus on human systems, epigenetic diseases that affect these systems and modes of treating epigenetic-based disorders and diseases. The intent of this book is to provide a stand-alone comprehensive volume that will cover all human systems relevant to epigenetic maladies and all major aspects of medical epigenetics. The overall goal is to provide the leading book on medical epigenetics that will be useful not only to physicians, nurses, medical students and many others directly involved with health care, but also investigators in life sciences, biotech companies, graduate students and many others who are interested in more applied aspects of epigenetics. Research in the area of translational epigenetics is a cornerstone of this volume. Critical reviews dedicated to the burgeoning role of epigenetics in medical practice Coverage of emerging topics including twin epigenetics as well as epigenetics of gastrointestinal disease, muscle disorders, endocrine disorders, ocular medicine, pediatric diseases, sports medicine, noncoding RNA therapeutics, pain management and regenerative medicine Encompasses a disease-oriented perspective of medical epigenetics as well as diagnostic and prognostic epigenetic approaches to applied medicine
Author: Alexis Jo Lawton (Ph.D.) Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
Reversible protein acetylation is a widespread, regulatory modification that was first discovered on histone proteins, but since has been described throughout the cell in major subcellular compartments. Several functional roles of lysine acetylation have been described including regulating enzymatic activity, protein-protein interactions, protein-DNA interactions, protein stability, and subcellular localization. With the improvements in mass spectrometry technologies, the number of identified acetylated sites has grown exponentially creating a new challenge to determine which sites are functional versus spurious. Furthermore, studies investigating the mechanisms regulating protein acetylation have primarily focused on histone acetylation, but how they may regulate non-histone protein acetylation has not been fully understood. Of importance, one of the main mechanisms through which acetylation may be regulated is through changes in the availability of acetyl-CoA. Since most acetyltransferases, including p300/CBP, have a Km near the estimated cellular concentrations of acetyl-CoA, manipulating concentrations of the substrate through activation of its production can have major effects on the levels of acetylation. Several studies have investigated the effect of knockdowns of acetyl-CoA generating enzymes (such as ACLY or ACSS2), however, few studies have measured the effects on non-histone protein acetylation. My research uses two main approaches to respond to these challenges in the field: 1) a robust, mass spectrometry method to quantify acetylation stoichiometry across the proteome, and 2) investigating dynamics of acetylation in response to changes in acetyl-CoA availability. Understanding both stoichiometry and dynamic responses during cellular stimulation are key features of interrogating the role of protein acetylation at a site- and protein-specific level. My research aims to understand the cellular mechanisms that are controlling dynamic acetylation in response to metabolic changes in acetyl Coenzyme A (acetyl-CoA) availability and production.Here, I examine the dynamics of global protein acetylation and the mechanisms regulating growth-factor stimulated dynamics, with a particular focus on how metabolism and the production and availability of acetyl-CoA is a major regulator of protein acetylation. Chapter 1 introduces protein acetylation and discusses the field's current understanding of acetylation functions, the quantitative techniques for investigating global non-histone protein acetylation, and the mechanisms involved in regulating protein acetylation. Chapter 2 presents an updated method for quantifying lysine acetylation stoichiometry and applies this method to identify serum-stimulated protein acetylation dynamics. Chapter 3 presents an in-progress project that examines the key regulatory nodes controlling growth-factor stimulated acetylation, including the kinase AKT, acetyl-CoA production through ACLY, and citrate export by SLC25A1. Chapter 4 presents the ongoing experiments and future directions for these projects. My thesis work has also allowed me to be a part of some critical collaborations that have investigated the connection between metabolism, acetyl-CoA availability, and protein acetylation in several interesting mouse models. My contributions and our key findings are presented in the Appendixes of this thesis. Appendix 1 investigates the effect of the dysregulation of the ER-localized acetyl-CoA transporter, AT-1, on protein acetylation. Appendix 2 investigates the effect of increased flux of citrate into the cytoplasm and nucleus through overexpression models of the extracellular membrane citrate transporter, SLC13A5, and the mitochondrial citrate transporter, SLC25A1, and their effect on protein acetylation. Appendix 3 investigates the effect of age, diet, and the loss of the mitochondrial localized deacetylase, SIRT3, on mitochondrial protein acetylation. Lastly, Appendix 4 examines the effects of the loss of individual ER-localized acetyltransferases, ATase1 and ATase2, on secretory pathway protein acetylation.
Author: Oscar Alzate Publisher: CRC Press ISBN: 1420076264 Category : Medical Languages : en Pages : 356
Book Description
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Author: Tso-Pang Yao Publisher: Springer Science & Business Media ISBN: 3642216315 Category : Medical Languages : en Pages : 269
Book Description
The book highlights work from many different labs that taught us abnormal HDACs potentially contribute to the development or progression of many human diseases including immune dysfunctions, heart disease, cancer, memory impairment, aging, and metabolic disorders.
Author: Gregory R. Bock
Author: Gregory R. Bock
Author: Robert M. Brosh
Author: T. Shantha Raju
Author: Tanveer Ali Dar
Author: William P. Jencks
Author: Alexis Jo Lawton (Ph.D.)
Author: Trygve Tollefsbol
Author: Alexis Jo Lawton (Ph.D.)
Author: Oscar Alzate
Author: Tso-Pang Yao