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Author: Corinna R. Hess Publisher: ISBN: Category : Amine oxidase Languages : en Pages : 194
Book Description
Amine oxidases (AO) are copper-containing enzymes that catalyze the conversion of amines to aldehydes. The active site is deeply buried within the protein network, a factor which has precluded many investigations into the mechanism. A series of electron tunneling molecular wires has been synthesized, designed to access the cofactors of amine oxidase by means of the substrate channel. These wires are comprised of phenyl-ethynyl units and thus promote rapid rates of electron transfer to the active site. An amine group attached to one end of the oligomer serves as the channel-specific functionality, which binds within the channel. Binding of the oligomers to AO was demonstrated through inhibition and fluorescence quenching studies. A variety of reporter groups has been attached to the chain end opposite the amine group, allowing us to probe the enzyme using a variety of techniques. A Re terminated complex was designed for electron transfer studies with the enzyme. The photophysics of this complex were investigated in the presence of AO. A thiol terminated wire also has been synthesized, which was employed in electrochemical studies of the amine oxidase cofactors. These studies provide evidence for the ability to communicate with the active site using the molecular wires. In addition, these complexes fulfilled our primary goal and enabled us to determine a redox potential of the TPQ cofactor.
Author: Corinna R. Hess Publisher: ISBN: Category : Amine oxidase Languages : en Pages : 194
Book Description
Amine oxidases (AO) are copper-containing enzymes that catalyze the conversion of amines to aldehydes. The active site is deeply buried within the protein network, a factor which has precluded many investigations into the mechanism. A series of electron tunneling molecular wires has been synthesized, designed to access the cofactors of amine oxidase by means of the substrate channel. These wires are comprised of phenyl-ethynyl units and thus promote rapid rates of electron transfer to the active site. An amine group attached to one end of the oligomer serves as the channel-specific functionality, which binds within the channel. Binding of the oligomers to AO was demonstrated through inhibition and fluorescence quenching studies. A variety of reporter groups has been attached to the chain end opposite the amine group, allowing us to probe the enzyme using a variety of techniques. A Re terminated complex was designed for electron transfer studies with the enzyme. The photophysics of this complex were investigated in the presence of AO. A thiol terminated wire also has been synthesized, which was employed in electrochemical studies of the amine oxidase cofactors. These studies provide evidence for the ability to communicate with the active site using the molecular wires. In addition, these complexes fulfilled our primary goal and enabled us to determine a redox potential of the TPQ cofactor.
Author: Astrid Sigel Publisher: John Wiley & Sons ISBN: 0470028149 Category : Science Languages : en Pages : 678
Book Description
Helmut Sigel, Astrid Sigel and Roland K.O. Sigel, in close cooperation with John Wiley & Sons launch a new Series “Metal Ions in Life Sciences”. There exists a whole range of books on Cytochromes P450, but none with the focus of this volume. This new volume in the Series concentrates on current hot topics in the area and tries to work out the underlying common developments. As a result the reader will find a systematic account of new results in this exciting research area. The table of contents gives an idea on the wide span of chapters, starting with overviews and the presentation of specific systems, and ending with chapters on carbon-carbon bond cleavage by P450 sytems, drug metabolism as catalyzed by P450 systems, decomposition of xenobiotics by P450 enzymes and design and engineering of new P450 systems.
Author: Rudolf K. Allemann Publisher: Royal Society of Chemistry ISBN: 0854041222 Category : Science Languages : en Pages : 412
Book Description
In recent years, there has been an explosion in knowledge and research associated with the field of enzyme catalysis and H-tunneling. Rich in its breath and depth, this introduction to modern theories and methods of study is suitable for experienced researchers those new to the subject. Edited by two leading experts, and bringing together the foremost practitioners in the field, this up-to-date account of a rapidly developing field sits at the interface between biology, chemistry and physics. It covers computational, kinetic and structural analysis of tunnelling and the synergy in combining these methods (with a major focus on H-tunneling reactions in enzyme systems). The book starts with a brief overview of proton and electron transfer history by Nobel Laureate, Rudolph A. Marcus. The reader is then guided through chapters covering almost every aspect of reactions in enzyme catalysis ranging from descriptions of the relevant quantum theory and quantum/classical theoretical methodology to the description of experimental results. The theoretical interpretation of these large systems includes both quantum mechanical and statistical mechanical computations, as well as simple more approximate models. Most of the chapters focus on enzymatic catalysis of hydride, proton and H" transfer, an example of the latter being proton coupled electron transfer. There is also a chapter on electron transfer in proteins. This is timely since the theoretical framework developed fifty years ago for treating electron transfers has now been adapted to H-transfers and electron transfers in proteins. Accessible in style, this book is suitable for a wide audience but will be particularly useful to advanced level undergraduates, postgraduates and early postdoctoral workers.
Author: Kenji Kano Publisher: Springer Nature ISBN: 9811589607 Category : Science Languages : en Pages : 135
Book Description
This book covers the fundamental aspects of the electrochemistry and redox enzymes that underlie enzymatic bioelectrocatalysis, in which a redox enzyme reaction is coupled with an electrode reaction. Described here are the basic concept and theoretical aspects of bioelectrocatalysis and the various experimental techniques and materials used to study and characterize related problems. Also included are the various applications of bioelectrocatalysis to bioelectrochemical devices including biosensors, biofuel cells, and bioreactors. This book is a unique source of information in the area of enzymatic bioelectrocatalysis, approaching the subject from a cross-disciplinary point of view.
Author: Corinna R. Hess
Author: Corinna R. Hess
Author: Electrochemical Society
Author: 
Author: Astrid Sigel
Author: Robert Maurice George Hynson
Author: 
Author: Mårten Wikström
Author: E. Buncel
Author: Rudolf K. Allemann
Author: Kenji Kano