Practical Enzymology PDF Download

Author: Hans Bisswanger
Publisher: John Wiley & Sons
ISBN: 3527820809
Category : Science
Languages : en
Pages : 574

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Book Description
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.

Author: Hans Bisswanger
Publisher: John Wiley & Sons
ISBN: 352734604X
Category : Science
Languages : en
Pages : 419

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Book Description
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.

Author: Hans Bisswanger
Publisher: John Wiley & Sons
ISBN: 3527659242
Category : Science
Languages : en
Pages : 362

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Book Description
The second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. www.wiley-vch.de/home/enzymology: A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter.

Author: Robert A. Copeland
Publisher: John Wiley & Sons
ISBN: 0471461857
Category : Science
Languages : en
Pages : 416

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Book Description
Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research.

Author: Lawrence C. Kuo
Publisher: Academic Press
ISBN: 0123812747
Category : Medical
Languages : en
Pages : 662

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Book Description
There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD. Also elaborated by experienced researchers in FBDD are sample preparations of fragments, proteins, and GPCR as well as examples of how to generate leads from hits. Offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in FBDD

Author: N.S. Punekar
Publisher: Springer
ISBN: 9811307857
Category : Science
Languages : en
Pages : 560

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Book Description
This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 0123809258
Category : Science
Languages : en
Pages : 915

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Book Description
Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. - Historical analysis of kinetic principles including advanced enzyme science - Provides both theoretical and practical measurements tools - Coverage of single molecular kinetics - Examination of force generation mechanisms - Discussion of organic and inorganic enzyme reactions

Author: Hyone-Myong Eun
Publisher: Elsevier
ISBN: 008053113X
Category : Science
Languages : en
Pages : 727

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Book Description
Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. - Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology - Presents information in an easily accessible format to serve as a quick reference source - Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques

Author: Haruo Suzuki
Publisher: CRC Press
ISBN: 1000681432
Category : Medical
Languages : en
Pages : 209

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Book Description
The first edition of this book covered the basic treatment of the enzyme reaction using the overall reaction kinetics and stopped-flow method, the general properties of protein and cofactors, the control of enzyme reaction, and the preparation of enzyme protein. These topics are the basis of enzyme research and thus suitable for the beginner in the field. The second edition presents the cofactors produced via the post-translational modification of the enzyme’s active site. These cofactors expand the function of enzymes and open a new research field. The carbonyl reagent phenylhydrazine and related compounds have been useful in finding some of the newly discovered cofactors and thus have been discussed in this edition. The topic of the control of enzyme activity through the channel of substrates and products in polyfunctional enzymes has also been expanded in this book.

Author: Jeannine Yon-Kahn
Publisher: Springer Science & Business Media
ISBN: 3642012299
Category : Science
Languages : en
Pages : 809

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Book Description
Molecular and Cellular Enzymology addresses not only experienced enzymologists but also students, teachers and academic and industrial researchers who are confronted with enzymological problems during their fundamental or applied research. In this field there is an urgent need for training in order to meet the requirements of both research and industrial endeavours. This book consists of several levels. Practical aspects and elementary explanations are given for the benefit of non-specialists’ and students’ understanding. In order to facilitate the task of students, two typographies have been adopted. The main text corresponds to basic knowledge, whereas text in a smaller font provides more specialised information. Specialists will also find topics more deeply expounded with the principal bibliographic references cited. The bibliography, however, is not exhaustive; the choice includes general books and review articles as well as some specialised articles. In this book, for the first time, the different molecular and cellular aspects of enzymology are presented together. Until now, there has been no book available in which these different aspects are treated in the same volume. In addition, besides the theoretical developments, this book provides a wealth of practical information for experimentalists.