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Author: Yulia Tsifansky-Zarchin Publisher: ISBN: Category : Languages : en Pages : 126
Book Description
"The ability of enzymes to fold into unique structures drives their remarkable catalytic activity. Recently, a great effort is done in order to duplicate this high activity of natural enzymes by using simple molecules, for instance amphiphilic peptides, which can self-assemble into different structures in water, creating microenvironments that emulate the active site of enzymes. In addition, the controlled assembly of amphiphilic peptides governs their self-replicating propensity, as it promotes their own formation by self-recognition of building blocks, as was previously shown by our group. The addition of functional groups to the selfreplicating peptides may provide bi-functional systems, which will be able to selfreplicate and perform enhanced catalysis, benefiting one another (Figure 1). In this study, we have first used short dipeptides and further synthesized longer amphiphilic peptides that can readily self-assemble into elongated fibrils and catalyse hydrolysis of thioesters.. The supramolecular architectures formed by the selfassembly of the peptides was characterized using battery of techniques. The peptides were tested for the esterase-like activity using different substrates, and the difference between ester and thioester hydrolysis was tested. We observed that Fmoc-FH appears to catalyze the hydrolysis reaction of the thioester substrate better than the oxyester substrate. For control, we used molecules such as acetyl Histidine (aH) as a nonaggregating analogue. We found the the designed catalyst enhancing the catalysis as twice as fast than the control reaction. We were able to provide insights into the factors that dictate the efficiency of the catalytic reaction, and in particular to demonstrate the role of the self-assembly property upon the catalytic activity. We believe that this research will get us closer to the ability of assembling an autocatalytic-replication cycle that will carry out the both functions as well as benefiting each other. "-- abstract.
Author: Yulia Tsifansky-Zarchin Publisher: ISBN: Category : Languages : en Pages : 126
Book Description
"The ability of enzymes to fold into unique structures drives their remarkable catalytic activity. Recently, a great effort is done in order to duplicate this high activity of natural enzymes by using simple molecules, for instance amphiphilic peptides, which can self-assemble into different structures in water, creating microenvironments that emulate the active site of enzymes. In addition, the controlled assembly of amphiphilic peptides governs their self-replicating propensity, as it promotes their own formation by self-recognition of building blocks, as was previously shown by our group. The addition of functional groups to the selfreplicating peptides may provide bi-functional systems, which will be able to selfreplicate and perform enhanced catalysis, benefiting one another (Figure 1). In this study, we have first used short dipeptides and further synthesized longer amphiphilic peptides that can readily self-assemble into elongated fibrils and catalyse hydrolysis of thioesters.. The supramolecular architectures formed by the selfassembly of the peptides was characterized using battery of techniques. The peptides were tested for the esterase-like activity using different substrates, and the difference between ester and thioester hydrolysis was tested. We observed that Fmoc-FH appears to catalyze the hydrolysis reaction of the thioester substrate better than the oxyester substrate. For control, we used molecules such as acetyl Histidine (aH) as a nonaggregating analogue. We found the the designed catalyst enhancing the catalysis as twice as fast than the control reaction. We were able to provide insights into the factors that dictate the efficiency of the catalytic reaction, and in particular to demonstrate the role of the self-assembly property upon the catalytic activity. We believe that this research will get us closer to the ability of assembling an autocatalytic-replication cycle that will carry out the both functions as well as benefiting each other. "-- abstract.
Author: Publisher: Elsevier ISBN: 0443236682 Category : Science Languages : en Pages : 550
Book Description
Peptide Catalysts, including Catalytic Amyloids, Volume 697 in this esteemed series, highlights new advances in the field, with this new volume presenting interesting topics on Screening of oxidative behaviors in catalytic amyloid assemblies, Catalytic amyloids derived for natural proteins, AFM-IR studies of catalytic amyloids, MD structural studies of catalytic amyloids, Characterization of crystalline, amyloid-like amino acid assemblies, Computational modeling of supramolecular peptide assemblies, and Assembly and activity of short prion-inspired peptides. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Updated release includes the latest information on Peptide Catalysts, including Catalytic Amyloids
Author: Mustafa O. Guler Publisher: Royal Society of Chemistry ISBN: 1839161159 Category : Science Languages : en Pages : 413
Book Description
Research and new tools in biomaterials development by using peptides are currently growing, as more functional and versatile building blocks are used to design a host of functional biomaterials via chemical modifications for health care applications. It is a field that is attracting researchers from across soft matter science, molecular engineering and biomaterials science. Covering the fundamental concepts of self-assembly, design and synthesis of peptides, this book will provide a solid introduction to the field for those interested in developing functional biomaterials by using peptide derivatives. The bioactive nature of the peptides and their physical properties are discussed in various applications in biomedicine. This book will help researchers and students working in biomaterials and biomedicine fields and help their understanding of modulating biological processes for disease diagnosis and treatments.
Author: Kyle Morris Publisher: ISBN: Category : Languages : en Pages :
Book Description
Self-assembling molecules are central to a plethora of processes found in nature, biotechnology and even disease. The importance of the non-covalent interaction of monomers to the formation of fibrillar assemblies is evident in the repeated use of this mechanism throughout nature, from essential cellular processes such as the formation of the cytoskeleton to the production of silk. Further, it has been recognised in the last two decades that a self-assembly mechanism, that is the formation of amyloid, underpins the pathology of protein misfolding diseases; it is therefore essential to dissect these mechanisms. Despite recent technological and model system developments, self-assembling molecules remain challenging to investigate. Using combined structural and biophysical characterisations of penta- and hexa-peptide self-assembling model systems these investigations shed further light on the structure of amyloid-like fibrils. The elucidation of the structures of these fibrillar systems not only has implications for disease but also makes them well placed for consideration for biotechnological applications. In reflecting upon how cross-ß structural architectures can be organised in the fibrillar state, a molecular and supramolecular model of fibrils formed by a fragment of!-synuclein is reported. The fibrils are found to consist of a novel and elaborate cross-ß architecture that leads to a helical supramolecular assembly spanning length scales previously unobserved for such a system. Where self-assembly is a useful route to supramolecular structure formation, the use of low molecular weight gelator (LMWG) peptides to create fibrillar structures with defined material properties is also explored. The complex link between molecular structure, self-assembled architecture, fibril formation, fibril interaction and ultimately bulk material properties is described. It is found that the determinants of self-assembly are distinct from the determinants of gelation and so future LMWG design will have to consider both individually. This work presents methodological advances in the characterisation of self-assembled structures. The investigations presented here have relevance for disease related processes but also to the technological use of these systems as materials. Finally, this work emphasises the beauty of the extravagant, yet elegant connection between molecular interaction and supramolecular selfassembly.
Author: Ayumi Imayoshi Publisher: Springer ISBN: 9789811674334 Category : Science Languages : en Pages : 0
Book Description
This book proposes a novel concept for molecular recognition. In the field of asymmetric synthesis approaching the mature science, asymmetric discrimination and catalytic synthesis of chiral supramolecules still stand as unsolved problems. The extreme difficulty in asymmetric synthesis of such supramolecules may result from the mobile nature of supramolecular chirality. Here the author shows the first highly enantioselective synthesis of mechanically chiral supramolecules. In the presence of a chiral organocatalyst, a mechanically planar chiral rotaxane was obtained with p erfect enantiopurity (>99% ee) with an excellent selectivity. The dynamic and flexible recognition mode enabled asymmetric synthesis of supramolecules with conformational flexibility and mobility. The recognition mode of the catalyst is a contrast to the traditional static and rigid recognition mode of the typical conventional catalysts. The concept of dynamic molecular recognition will be adopted as a novel concept in a wide range of fields beyond the field of organic chemistry, including material chemistry, biochemistry, and medicinal chemistry.
Author: Vibin Ramakrishnan Publisher: Academic Press ISBN: 0323985432 Category : Science Languages : en Pages : 297
Book Description
De novo Peptide Design: Principles and Applications presents the latest developments in the fields of therapeutic peptides and bio-nanotechnology. The title focuses on the design of peptides, particularly how peptides may be tailored to specific functions. It includes computational and experimental protocols to assist in the design of peptides. Sections cover the basics of protein and peptide structure, modeling and simulation, solid phase peptide synthesis, peptide-based antibiotics, drug delivery, peptide nanomaterials, aromatic interactions directing nano-assembly, protein/peptide aggregation, therapeutic interventions against protein/peptide aggregation diseases, peptide based hydrogels, computational tools and algorithms for peptide design, and experimental protocols in peptide chemistry. In addition, the book covers key aspects in peptide design, providing a solution for researchers working within the 'peptidic universe' to create new therapeutic agents. Gives comprehensive coverage, including peptide design, modeling, synthesis and applications Presents emerging topics in the design of peptide-based therapeutics Details the latest developments in the fields of therapeutic peptides and bio-nanotechnology Considers peptide design and the tailoring of peptides to specific functions Offers computational tools and algorithms for peptide design and experimental protocols for peptide chemistry
Author: Charalampos Pappas Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
Responsiveness, functionality and adaptability are further demonstrated using different stimuli. Biocatalytic self-assembly has been utilised to direct supramolecular systems. Thermodynamically driven biocatalytic self-assembly is used to achieve morphological control (fibres, sheets and tubes) on aromatic dipeptide amphiphiles, via minimal stereo-electronic substitution on the para position of the phenylalaline amino acid residue. The control of the resultant supramolecular nanostructures and properties arises from the relative importance of stacking interactions among the aromatics and H-bonding between the dipeptide backbones. The concept of thermodynamically driven biocatalytic self-assembly is further utilised to direct the formation of Dynamic Peptide Libraries (DPLs). The amino acids within a dipeptide sequence are exchanged dynamically using in situ catalytic synthesis and hydrolysis of amide bonds, where the free energy involved in selfassembly of the nanostructure provides the driving force for its formation. The use of searchable dynamic peptide libraries gives rise to the ability to explore the structural sequences space of short peptides. Selective catalytic amplification is achieved through an interplay with environmental triggers. Differential formation of peptide subunits (library members), accompanied with structural reconfiguration is favored in the presence of different environment, such as solvents and salts. Additionally, a direct comparison between biocatalytically driven formation of different molecular species and chemically synthesized assemblies is used to show that shape control may be achieved. Biocatalytic self-assembly is also used to trigger the formation of non-equilibrium transient supramolecular systems. In this case, structural adaption is achieved based on biocatalytic formation and hydrolysis of self-assembling tripeptides, which catalyzed by chymotrypsin, starting from a simple dipeptide methyl ester, the wellknown sweetener, aspartame. The chemical design dictates the kinetics and the consequent lifetime of the nanostructures formed, which can be refueled several times by the addition of the fuel (aspartame), where the fibres are continuously forming and shortening, indicating a dynamically unstable system. This thesis ends with a small chapter, where we demonstrate the use of audible sound frequencies (
Author: Andreas Walther Publisher: John Wiley & Sons ISBN: 3527822003 Category : Science Languages : en Pages : 448
Book Description
Out-of-Equilibrium (Supra)molecular Systems and Materials A must-have resource that covers everything from out-of-equilibrium chemical systems to active materials Out-of-Equilibrium (Supra)molecular Systems and Materials presents a comprehensive overview of the synthetic approaches that use molecular and supramolecular bonds in various out-of-equilibrium situations. With contributions from noted experts on the topic, the text contains information on the design of dissipative chemical systems that adapt their structures in space and time when fueled by an external source of energy. The contributors also examine molecules, nanoscale objects and materials that can produce mechanical work based on molecular machines. Additionally, the book explores living supramolecular polymers that can be trapped in kinetically stable states, as well as out-of-equilibrium chemical networks and oscillators that are important to understand the emergence of complex behaviors and, in particular, the origin of life. This important book: Offers comprehensive coverage of fields from design of out-of-equilibrium self-assemblies to molecular machines and active materials Presents information on a highly emerging and interdisciplinary topic Includes contributions from internationally renowned scientists Written for chemists, physical chemists, biochemists, material scientists, Out-of-Equilibrium (Supra)molecular Systems and Materials is an indispensable resource written by top scientists in the field.
Author: Toshiki Asao Publisher: BoD – Books on Demand ISBN: 9535132415 Category : Science Languages : en Pages : 296
Book Description
Amino Acid - New Insights and Roles in Plant and Animal provides useful information on new aspects of amino acid structure, synthesis reactions, dietary application in animals, and metabolism in plants. Section 1 includes chapters that describe the therapeutic uses, antiallergic effects, new aspects in the D-amino acid structure, historical background of desmosines, and stereoselective synthesis of ?-aminophosphonic acids. Section 2 presents the role of amino acids in plants, which includes new insights and aspects of D-amino acids, metabolism and transport in soybean, changes during energy storage compound accumulation of microalgae, and determination of amino acids from natural compounds. Section 3 describes the chapters on methodologies and requirement of dietary amino acids for Japanese quails, laying hens, and finishing pigs. The final chapter identifies potential importance of glutathione S-transferase activity for generating resistance to triclabendazole in Fasciola hepatica.
Author: Jennifer J. McManus Publisher: Humana ISBN: 9781493996803 Category : Science Languages : en Pages : 266
Book Description
This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Author: Yulia Tsifansky-Zarchin
Author: Yulia Tsifansky-Zarchin
Author: 
Author: Mustafa O. Guler
Author: Kyle Morris
Author: Ayumi Imayoshi
Author: Vibin Ramakrishnan
Author: Charalampos Pappas
Author: Andreas Walther
Author: Toshiki Asao
Author: Jennifer J. McManus