Optical Trapping in Microfluidic Channels for Functional and Chemical Analysis of Single Cells PDF Download

Author: Michael Werner
Publisher:
ISBN:
Category :
Languages : en
Pages : 97

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Author: Jin-Ming Lin
Publisher: Springer Nature
ISBN: 9813297298
Category : Science
Languages : en
Pages : 261

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Book Description
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Author: Takasi Nisisako
Publisher: MDPI
ISBN: 3039366947
Category : Technology & Engineering
Languages : en
Pages : 230

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Book Description
Microfluidic platforms are increasingly being used for separating a wide variety of particles based on their physical and chemical properties. In the past two decades, many practical applications have been found in chemical and biological sciences, including single cell analysis, clinical diagnostics, regenerative medicine, nanomaterials synthesis, environmental monitoring, etc. In this Special Issue, we invited contributions to report state-of-the art developments in the fields of micro- and nanofluidic separation, fractionation, sorting, and purification of all classes of particles, including, but not limited to, active devices using electric, magnetic, optical, and acoustic forces; passive devices using geometries and hydrodynamic effects at the micro/nanoscale; confined and open platforms; label-based and label-free technology; and separation of bioparticles (including blood cells), circulating tumor cells, live/dead cells, exosomes, DNA, and non-bioparticles, including polymeric or inorganic micro- and nanoparticles, droplets, bubbles, etc. Practical devices that demonstrate capabilities to solve real-world problems were of particular interest.

Author: Cesare Gargioli
Publisher: Frontiers Media SA
ISBN: 2889459985
Category :
Languages : en
Pages : 272

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Extracellular Matrix (ECM) has been considered for a long time merely a scaffold sustaining cell and tissue function. Despite this simplistic view shared by many, nowadays ECM and their mechanic-physical and chemical characteristic acquired a progressive larger role actively regulating cell life: survival, proliferation, gene expression and differentiation. The interplay between cells and the ECM is continuously controlled at the cell level in a dynamic way. While cells synthesize the raw components of the ECM, this in turn impacts on cell function by providing chemical, topographical and mechanical hints. Such stimuli have been proven to control several aspects of cell function, including survival, proliferation, differentiation and migration. The molecular pathways activated by cells in response to the physical cues arising from the ECM are being disclosed and thus the possibility to control cell function through materials design is becoming more realistic. Current in vitro protocols, relying in 2D cell culture system, entail reductionist approaches to the complexity of cell-ECM interaction and result in cells rapidly losing their distinctive functions in culture. Understanding and replicating the 3D microenvironmental cues affecting cell function appears as a mandatory requirement for the development of next-generation biomaterials, as well as for the establishment of more physiologically relevant and predictive in vitro models of diseases. Such an effort will require a multidisciplinary approach at the convergence of biophysics, biology, nanotechnology, and bioengineering.

Author: Albert Berg
Publisher: Springer Science & Business Media
ISBN: 1402028601
Category : Medical
Languages : en
Pages : 373

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Book Description
This volume is volume entirely dedicated to microfabricated cell-based systems. It will provide readers with a quick introduction to the field as well as with a variety of specific examples of such Lab-on-Chip systems for cellomics applications. It will give investigators inspiration for innovative research topics, whereas end users will be surprised about the wide variety of new and exciting applications.

Author: Andreas Manz
Publisher: Royal Society of Chemistry
ISBN: 1788019385
Category : Technology & Engineering
Languages : en
Pages : 307

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Book Description
Microfluidic technology is revolutionising a number of scientific fields, including chemistry, biology, diagnostics, and engineering. The ability to manipulate fluids and objects within networks of micrometre-scale channels allows reductions in processing and analysis times, reagent and sample consumption, and waste production, whilst allowing fine control and monitoring of chemical or biological processes. The integration of multiple components and processes enable “lab-on-a-chip” devices and “micro total analysis systems” that have applications ranging from analytical chemistry, organic synthesis, and clinical diagnostics to cell biology and tissue engineering. This concise, easy-to-read book is perfectly suited for instructing newcomers on the most relevant and important aspects of this exciting and dynamic field, particularly undergraduate and postgraduate students embarking on new studies, or for those simply interested in learning about this widely applicable technology. Written by a team with more than 20 years of experience in microfluidics research and teaching, the book covers a range of topics and techniques including fundamentals (e.g. scaling laws and flow effects), microfabrication and materials, standard operations (e.g. flow control, detection methods) and applications. Furthermore, it includes questions and answers that provide for the needs of students and teachers in the area.

Author: Luc Charron
Publisher:
ISBN: 9780494975534
Category :
Languages : en
Pages :

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Author: Jin-Ming Lin
Publisher: Springer
ISBN: 9811053944
Category : Science
Languages : en
Pages : 435

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Book Description
This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Author: Huibin Wei
Publisher: Springer Science & Business Media
ISBN: 3642323588
Category : Science
Languages : en
Pages : 117

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Book Description
This thesis describes a new approach for cell analysis by the rapid developing microfluidic technology. The nominee has made great contributions to develop a new analysis platform which combined microfluidic devices with mass spectrometry to determine the trace compounds secreted by cells. Based on this analysis platform, she studied the specific cell secreting behaviors under controlled microenvironment, of which the secretion compounds were qualified and semi-quantified by mass spectrometry. A novel cell sorting device integrated homogenous porous PDMS membrane was invented to classify cells from real samples based on the size difference. The nominee further studied the signal transmission between different cells, and the signal chemicals were qualitative and quantitative monitored by the analysis platform. This indicates the potential significant application of the new cell analysis platform in medicine screening and early diagnosis.

Author: Peter Rodgers
Publisher: World Scientific
ISBN: 9814466867
Category : Technology & Engineering
Languages : en
Pages : 367

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Book Description
This book contains 35 review articles on nanoscience and nanotechnology that were first published in Nature Nanotechnology, Nature Materials and a number of other Nature journals. The articles are all written by leading authorities in their field and cover a wide range of areas in nanoscience and technology, from basic research (such as single-molecule devices and new materials) through to applications (in, for example, nanomedicine and data storage).