Morpho-physiological Traits of Sorghum (Sorghum Bicolor (l.) Moench) Lines in Relation to Water Stress PDF Download

Author: C.L.Narasimha Rao
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ISBN:
Category :
Languages : en
Pages : 159

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Author: C. L. Narasimha Rao
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Category :
Languages : en
Pages : 17

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Author: Jerry R. Rice
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Category :
Languages : en
Pages : 322

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Author: Terry Wayne Barger
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Category : Sorghum
Languages : en
Pages : 134

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Author: Sidy B. Coulibaly
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Category :
Languages : en
Pages : 316

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Languages : en
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Grain sorghum (Sorghum bicolor L. Moench) is the fourth most important cereal crop grown throughout the semi-arid regions of the world. It is a staple food crop in Africa and Asia, while it is an important feed crop in the United States (US). More recently it is increasingly becoming important as a potential bioenergy feedstock crop around the world. The state of Kansas is the largest producer of grain sorghum in the US and contributes 40% of the total production. Drought is one of the major environmental factors limiting sorghum production in the semi-arid regions of the US, Asia and Africa. It is estimated that global crop losses due to drought stress exceed $10 billion annually. In crop production, drought stress can be classified into pre- or post-flowering. Even though the world collections of sorghum contain over 35,000 accessions, the genetic base currently used in breeding programs is very small (about 3%). Thus, it is important to identify diverse breeding lines for crop improvement. The diversity (association) panel consisting of 300 sorghum lines from all over the world was assembled for trait evaluation and association mapping. In this research these lines were grouped into the five major races (Figure 1) and 10 intermediate races of sorghum. The objectives of the research are to: (i) quantify the performance of the diversity panel under field conditions in Kansas, (ii) identify critical physiological traits affected by drought at both pre- and post-flowering stages of sorghum development, (iii) identify the most sensitive stage to drought stress during the reproductive phase of sorghum development and, (iv) test the feasibility of using a chlorophyll fluorescence assay (CVA) as a tool for identifying stay-green lines in grain sorghum during early stages of crop development. Field experiments were conducted in 2006 and 2007 in two locations in Kansas (Manhattan and Hays) under rain fed and irrigated conditions for the association panel. Objectives (iii) and (iv) were achieved with controlled environment experiments conducted in the greenhouse at the agronomy department, Kansas State University in 2006 and 2007. Results showed that there was large genetic variability among and within different races in the diversity panel for growth, physiological traits and yield components. Some genotypes showed yield stability across the different environments that were investigated. Drought significantly decreased seed number and harvest index across genotypes and races. In grain sorghum the period prior to flowering (panicle initiation) was the most sensitive stage to drought stress, in terms of its effect on seed-set, during reproductive development. A cell viability assay showed that there were significant differences in the loss of cell viability between leaf sample of stay green and non-stay green genotypes when leaf samples are collected in the morning and subjected to high respiratory demand. Therefore the chlorophyll fluorescence assay has potential as a tool for stay green trait screening at early stages of growth in grain sorghum.

Author: Mohankumar Heraganahally Kapanigowda
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Languages : en
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There is an increasing need to improve crop water-use efficiency (WUE) (ratio of whole-plant biomass to cumulative transpiration) due to decreased water availability and increased food and energy demands throughout the world. The objective of the study was to estimate the genetic variation and genetic basis for transpiration efficiency A:E (CO2 assimilation rate (A) divided by transpiration rate (E)) trait and its relationship to WUE related to pre-flower drought tolerance in recombinant inbred lines (RILs) of sorghum and associated QTLs. A greenhouse study was conducted at Bushland, TX, 2008, using 71 RILs derived from cross of Tx430 x Tx7078. A randomized complete block experimental design was used, with both genotype and water regime (40 and 80 percent water regime) as experimental factors, and four replications. Genotype had a significant effect on A, E and A:E under both the environments. Among the RILs, entry means for A:E ranged from 1.58 to 3.07 mmol CO2 mol^-1 H2O and 1.18 to 4.36 mmol CO2 mol^-1 H2O under 80 percent and 40 percent water regime, respectively. Heritability estimates based on individual environments for A:E, A and E were 0.77, 0.45 and 0.37 under 80 percent water regime and 0.90, 0.33 and 0.71 under 40 percent water regime, respectively. A genetic map was constructed by digital genotyping method using Illumina GAII sequencer with 261 informative indel/ single-nucleotide polymorphism (SNP's) markers distributed over 10 linkage groups. Three significant QTLs associated with transpiration efficiency were identified; two on SBI-09 and one on SBI-10 with one logarithmic of odds (LOD) interval length ranging from 5.3 to 5.7 cM and accounting for 17 percent - 21 percent of the phenotypic variation. In field and greenhouse evaluation of agronomic of traits at College Station and Halfway, TX, 91 QTL that control variation in six major agronomic traits such as plant height, flowering, biomass, leaf area, leaf greenness and stomatal density were identified. Co-localization of transpiration efficiency QTLs with agronomic traits such as leaf area, biomass, leaf width and stomatal density indicated that these agronomically important QTLs can be used for further improving the sorghum performance through marker assisted selection (MAS) under pre-flowering drought stress conditions.

Author: Karen Ruth Harris
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Category :
Languages : en
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Sorghum (Sorghum bicolor [L.] Moench) is the fifth most economically important cereal grown worldwide and is a source of food, feed, fiber and fuel. Sorghum, a C4 grass and a close relative to sugarcane, is adapted to hot, dry adverse environments. Some genotypes of sorghum called stay-green have delayed leaf senescence during grain ripening under drought stress conditions which allows normal grain filling whereas most sorghum lines senesce early under post-anthesis drought. Eight sources of stay-green have been identified in the sorghum germplasm collection, most originating from Sudan and Ethiopia. The diversity of the eight sources of staygreen was analyzed using 55 simple sequence repeats (SSR) markers with genome coverage. This analysis showed that the sources of stay-green are quite diverse and can be divided into five groups based on race or working group. Three sources of stay-green have been used to identify 12 major quantitative trait loci (QTL) that modulate this trait. The origin of favorable alleles for stay-green was traced backward to ancestral lines and forward into breeding materials derived from stay-green germplasm. The analysis of the origin of favorable alleles for stay-green helped explain why subsets of stay-green QTL were identified in different studies and provided evidence that there may be more than one favorable allele in the sorghum germplasm for several of the stay-green QTL. Analysis of stay-green breeding lines from three public sorghum-breeding programs revealed that one of the main QTL identified in mapping studies was not being used in the breeding programs (0/13), most likely due to its association with an allele for lemon yellow seeds. In addition, a subset of the regions containing favorable alleles for staygreen from the genotype BTx642 were over represented in stay-green breeding lines. Nearly isogenic lines containing favorable alleles from BTx642 for Stg1, Stg2, Stg3, and Stg4 in a RTx7000 (senescent) background were characterized and each NIL was shown to exhibit a stay-green phenotype. Based in part on this information, fine-mapping of Stg1 was undertaken by crossing the Stg1 NIL to RTx7000. Overall, these results revealed the origin of favorable alleles for stay-green and the current utilization of alleles for stay-green in public breeding programs. In addition, this study identified additional stay-green sources that could be used for further QTL analysis and highlighted the genetic complexity of the stay-green trait.

Author: Anuj Chiluwal
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Category :
Languages : en
Pages :

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Sorghum (Sorghum bicolor (L.) Moench) is one of the hardiest crop to abiotic stresses compared with other grain crops. However early stage chilling, terminal heat and drought stress are three most damaging abiotic stresses that have limited sorghum productivity in the US Great plains and other locations having similar environmental conditions. Three studies were conducted with an overall goal aimed at increasing grain sorghum's resilience to harsh climatic conditions. In the first study, four promising chilling stress tolerant sorghum advanced breeding lines, a known early stage chilling tolerant Chinese landrace (Shan Qui Red - SQR) and a susceptible US elite cultivar (RTx430) as checks were assessed for chilling tolerance during emergence and early growth under field and controlled environments. Aerial phenotyping using unmanned aircraft systems (UAS) fitted with multispectral camera was used to capture reflectance-based vegetation indices (NDVI and NDRE) in field experiments. Some advanced breeding lines with superior agronomic background also recorded significantly better emergence, seedling growth and vigor compared to SQR under chilling conditions. Aerial phenotyping indices from images taken between 30 and 60 days after emergence were consistently correlated with destructive measurements under early plantings, indicating their effectiveness in differentiating chilling responses. Second study was conducted to understand physiological mechanisms inducing heat stress resilience in sorghum during flowering. A diverse set of sorghum inbreds and selected hybrids were tested under greenhouse, growth chamber facilities and field conditions. A highly conserved early-morning-flowering mechanism was observed across all the inbreds and hybrids, with the peak anthesis wherein >90% of florets completed flowering within 30 min after dawn. The conserved response was consistent even under drought stress and heat stress exposure imposed at different times of the day. Our findings report a novel heat escaping early-morning-flowering mechanism effectively employed by sorghum to minimize heat stress impact at anthesis. Another experiment with sequential increase in daytime temperature treatments suggest heat stress induced loss in pollen viability to be a key factor resulting in reduced seed-set and grain yield. The findings suggest heat stress could have a greater impact on post-pollen germination processes such as fertilization, embryo formation and development. We identified a heat tolerant genotype "Macia" which appears to be a promising donor for developing improved heat tolerant sorghum hybrids. In the third study, a bi-parental recombinant inbred lines (RILs) mapping population developed from elite post flowering drought susceptible cultivar (RTx430) and a known drought tolerant cultivar (SC35) were evaluated under wide spectrum of environments and moisture conditions. Several novel and major QTL for grain yield, panicle neck diameter, effective quantum yield of photosystem II and chlorophyll content were identified. The genomic regions and the candidate genes within these regions can potentially help in improving source and sink dynamics in sorghum under diverse environments. The findings from these studies will complement ongoing efforts in developing future sorghum with enhanced resilience to different abiotic stresses that continue to limit sorghum productivity.

Author: Abdalla I. Sheikh-Mohamed
Publisher:
ISBN:
Category : Plants
Languages : en
Pages : 106

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