Author: Thomas Hutchinson
Publisher: Elsevier
ISBN: 0323150195
Category : Nature
Languages : en
Pages : 443
Book Description
Microprobe Analysis of Biological Systems covers the proceedings of the 1980 Microprobe Analysis of Biological Systems conference held at Battelle Conference Center in Seattle, Washington. Most of the major laboratories in the field of biological microanalysis in the United States, England, Scotland, France, and Germany are represented. The conference presents the findings, theories, techniques, and procedures of the laboratory represented, no matter how tentative and exploratory. This book is divided into four parts encompassing 22 chapters that focus on biological applications of microprobe analysis. The introductory part describes the application of electron microprobe and X-ray microanalyses in studies of epithelial transport, avian salt gland, electrolyte transport, and acrosome reaction. The subsequent part covers the application of microprobe techniques in the analysis of cardiac, skeletal, vascular smooth, and freeze-dried muscles. It also describes a method for obtaining erythrocyte preparations for validating biological microprobe methods and the continuum-fluorescence effect on thick biological tissue. The method using freeze-substitution to localize calcium in quick-frozen tissue for X-ray microanalysis is also explained. The third part of the book tackles the principles, basic features, and applications of electron energy-loss spectroscopy. Discussions on the use of inner-shell signals for a quantitative local microanalysis technique; theoretical study of the energy resolution; and collection efficiency of a magnetic spectrometer are also included. The final part covers the elemental distribution in single erythrocytes using X-ray microanalysis. It also discusses the fundamentals of cryosectioning process for X-ray microanalysis of diffusible elements and the freezing behavior of a number of chemically different gels chosen for their partial resemblance to biological structures. Considerable chapters contain materials and methods, results, discussions, conclusions, and references. This book will be of value to scientists interested in elemental and ion transport within cells and between cells and extracellular compartments.
Microprobe Analysis of Biological Systems
Author: Thomas Hutchinson
Publisher: Elsevier
ISBN: 0323150195
Category : Nature
Languages : en
Pages : 443
Book Description
Microprobe Analysis of Biological Systems covers the proceedings of the 1980 Microprobe Analysis of Biological Systems conference held at Battelle Conference Center in Seattle, Washington. Most of the major laboratories in the field of biological microanalysis in the United States, England, Scotland, France, and Germany are represented. The conference presents the findings, theories, techniques, and procedures of the laboratory represented, no matter how tentative and exploratory. This book is divided into four parts encompassing 22 chapters that focus on biological applications of microprobe analysis. The introductory part describes the application of electron microprobe and X-ray microanalyses in studies of epithelial transport, avian salt gland, electrolyte transport, and acrosome reaction. The subsequent part covers the application of microprobe techniques in the analysis of cardiac, skeletal, vascular smooth, and freeze-dried muscles. It also describes a method for obtaining erythrocyte preparations for validating biological microprobe methods and the continuum-fluorescence effect on thick biological tissue. The method using freeze-substitution to localize calcium in quick-frozen tissue for X-ray microanalysis is also explained. The third part of the book tackles the principles, basic features, and applications of electron energy-loss spectroscopy. Discussions on the use of inner-shell signals for a quantitative local microanalysis technique; theoretical study of the energy resolution; and collection efficiency of a magnetic spectrometer are also included. The final part covers the elemental distribution in single erythrocytes using X-ray microanalysis. It also discusses the fundamentals of cryosectioning process for X-ray microanalysis of diffusible elements and the freezing behavior of a number of chemically different gels chosen for their partial resemblance to biological structures. Considerable chapters contain materials and methods, results, discussions, conclusions, and references. This book will be of value to scientists interested in elemental and ion transport within cells and between cells and extracellular compartments.
Publisher: Elsevier
ISBN: 0323150195
Category : Nature
Languages : en
Pages : 443
Book Description
Microprobe Analysis of Biological Systems covers the proceedings of the 1980 Microprobe Analysis of Biological Systems conference held at Battelle Conference Center in Seattle, Washington. Most of the major laboratories in the field of biological microanalysis in the United States, England, Scotland, France, and Germany are represented. The conference presents the findings, theories, techniques, and procedures of the laboratory represented, no matter how tentative and exploratory. This book is divided into four parts encompassing 22 chapters that focus on biological applications of microprobe analysis. The introductory part describes the application of electron microprobe and X-ray microanalyses in studies of epithelial transport, avian salt gland, electrolyte transport, and acrosome reaction. The subsequent part covers the application of microprobe techniques in the analysis of cardiac, skeletal, vascular smooth, and freeze-dried muscles. It also describes a method for obtaining erythrocyte preparations for validating biological microprobe methods and the continuum-fluorescence effect on thick biological tissue. The method using freeze-substitution to localize calcium in quick-frozen tissue for X-ray microanalysis is also explained. The third part of the book tackles the principles, basic features, and applications of electron energy-loss spectroscopy. Discussions on the use of inner-shell signals for a quantitative local microanalysis technique; theoretical study of the energy resolution; and collection efficiency of a magnetic spectrometer are also included. The final part covers the elemental distribution in single erythrocytes using X-ray microanalysis. It also discusses the fundamentals of cryosectioning process for X-ray microanalysis of diffusible elements and the freezing behavior of a number of chemically different gels chosen for their partial resemblance to biological structures. Considerable chapters contain materials and methods, results, discussions, conclusions, and references. This book will be of value to scientists interested in elemental and ion transport within cells and between cells and extracellular compartments.
Electron Probe Microanalysis
Author: Karl Zierold
Publisher: Springer Science & Business Media
ISBN: 364274477X
Category : Science
Languages : en
Pages : 331
Book Description
The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Publisher: Springer Science & Business Media
ISBN: 364274477X
Category : Science
Languages : en
Pages : 331
Book Description
The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Microprobe Analysis in Biology and Medicine
Author: Patrick Echlin
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 400
Book Description
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 400
Book Description
Microbeam Analysis in Biology
Author: Claude Lechene
Publisher: Elsevier
ISBN: 0323143342
Category : Nature
Languages : en
Pages : 695
Book Description
Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology.
Publisher: Elsevier
ISBN: 0323143342
Category : Nature
Languages : en
Pages : 695
Book Description
Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology.
Microprobe Analysis of Biological Sistems
Author: Thomas E. Hutchinson
Publisher:
ISBN:
Category :
Languages : en
Pages : 427
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 427
Book Description
High Resolution Scanning Electron Microprobe Analysis of Biological Material
Biomedical Applications of Microprobe Analysis
Author: Peter Ingram
Publisher: Academic Press
ISBN: 0080524567
Category : Science
Languages : en
Pages : 573
Book Description
Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter
Publisher: Academic Press
ISBN: 0080524567
Category : Science
Languages : en
Pages : 573
Book Description
Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter
Electron Probe Microanalysis in Biology
Author: David A. Erasmus
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 288
Book Description
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 288
Book Description
Research Grants Index
Author: National Institutes of Health (U.S.). Division of Research Grants
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1074
Book Description
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1074
Book Description
Microprobe Analysis in Medicine
Author: Peter Ingram
Publisher:
ISBN:
Category : Diagnosis, Electron microscopic
Languages : en
Pages : 388
Book Description
A work reviewing the applications of new microprobe analysis techniques in medicine. Authoritative chapters illustrate a variety of techniques and demonstrate appropriate applications to specific diseases. Key coverage includes pulmonary pathology and asbestoses.
Publisher:
ISBN:
Category : Diagnosis, Electron microscopic
Languages : en
Pages : 388
Book Description
A work reviewing the applications of new microprobe analysis techniques in medicine. Authoritative chapters illustrate a variety of techniques and demonstrate appropriate applications to specific diseases. Key coverage includes pulmonary pathology and asbestoses.