Methods of Enzymatic Analysis: Fundamentals PDF Download

Author: Hans Ulrich Bergmeyer
Publisher:
ISBN:
Category : Enzymatic analysis
Languages : en
Pages : 608

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Author: Hans-Ulrich Bergmeyer
Publisher: Wiley-Blackwell
ISBN: 9783527260416
Category : Science
Languages : en
Pages : 574

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Author: Edward F. Rossomando
Publisher: John Wiley & Sons
ISBN: 0470111003
Category : Science
Languages : en
Pages : 480

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The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.

Author: Athel Cornish-Bowden
Publisher: Elsevier
ISBN: 1483161196
Category : Science
Languages : en
Pages : 245

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Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.

Author:
Publisher:
ISBN: 9780895732316
Category :
Languages : en
Pages : 0

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Author: Hans Ulrich Bergmeyer
Publisher:
ISBN:
Category : Biochemistry
Languages : en
Pages : 1102

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Author: Hans Ulrich Bergmeyer
Publisher:
ISBN:
Category : Enzymatic analysis
Languages : en
Pages : 594

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Book Description

Author: Hans Ulrich Bergmeyer
Publisher:
ISBN: 9780895732316
Category :
Languages : en
Pages : 0

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Author: Rickey Y. Yada
Publisher: Elsevier
ISBN: 1782422978
Category : Technology & Engineering
Languages : en
Pages : 266

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Improving and Tailoring Enzymes for Food Quality and Functionality provides readers with the latest information on enzymes, a biological processing tool that offers the food industry a unique means to control and tailor specific food properties. The book explores new techniques in the production, engineering, and application of enzymes, covering sourcing, isolation, and production of enzymes for food applications. In addition, chapters include detailed discussions of enzyme processing, analytical and diagnostic applications of enzymes in the food industry, and enzyme applications in specific food commodities. - Provides readers with the latest information on enzymes and their unique applications in the food industry - Explores new techniques in the production, engineering, and application of enzymes, covering sourcing, isolation, and production of enzymes for food applications - Chapters include detailed discussions of enzyme processing, engineering and analytical and diagnostic applications of enzymes in the food industry, and enzyme applications in specific food commodities

Author: Hans-UIrich Bergmeyer
Publisher: Elsevier
ISBN: 0323161375
Category : Science
Languages : en
Pages : 640

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Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases. Part III discusses hydrolases including esterases, glycoside hydrolases, peptidases, and proteinases, whereas Part IV looks at lyases, isomerases, and ligases, such as fructose-1, 6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase. This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.