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Author: Rony Seger Publisher: Springer Science & Business Media ISBN: 1592596711 Category : Science Languages : en Pages : 335
Book Description
Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals. These cascades operate as major lines of communication within a complicated signaling network that regulates many cellular processes, including proliferation, differentiation, development, stress response, and apoptosis. More than 15,000 papers on MAPKs have been published over the past few years, with the number of publications increasing each year. More and more laboratories embark on the study of MAPK cascades in many d- tinct cellular systems and in particular their role in disease. Future challenges in the study of MAPK cascades remain in understa- ing the role of the various components and isoforms of the cascades in the multiple critical functions that they regulate in the whole organism, as well as the diseases caused by their malfunction. Data from gene-disrupted mice s- gest that inhibition of the MAPK cascades may have serious consequences on the development and growth of the animals. For example, targeted deletion of MEK1 is lethal, owing to developmental problems of placental vasculature and abnormal fibroblast migration. This lethality occurs in spite of the normal expression of MEK2, indicating that although the two MEK isoforms are apparently similar, they do have distinct functions, at least during embryog- esis. The ERK cascade was also shown to play a central role in brain function and in learning and memory.
Author: Rony Seger Publisher: Springer Science & Business Media ISBN: 1592596711 Category : Science Languages : en Pages : 335
Book Description
Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals. These cascades operate as major lines of communication within a complicated signaling network that regulates many cellular processes, including proliferation, differentiation, development, stress response, and apoptosis. More than 15,000 papers on MAPKs have been published over the past few years, with the number of publications increasing each year. More and more laboratories embark on the study of MAPK cascades in many d- tinct cellular systems and in particular their role in disease. Future challenges in the study of MAPK cascades remain in understa- ing the role of the various components and isoforms of the cascades in the multiple critical functions that they regulate in the whole organism, as well as the diseases caused by their malfunction. Data from gene-disrupted mice s- gest that inhibition of the MAPK cascades may have serious consequences on the development and growth of the animals. For example, targeted deletion of MEK1 is lethal, owing to developmental problems of placental vasculature and abnormal fibroblast migration. This lethality occurs in spite of the normal expression of MEK2, indicating that although the two MEK isoforms are apparently similar, they do have distinct functions, at least during embryog- esis. The ERK cascade was also shown to play a central role in brain function and in learning and memory.
Author: George Komis Publisher: Humana ISBN: 9781493945894 Category : Science Languages : en Pages : 0
Book Description
Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students.
Author: Heribert Hirt Publisher: Springer Science & Business Media ISBN: 354049166X Category : Science Languages : en Pages : 237
Book Description
Mitogen-activated protein kinase (MAPK) pathways are modules involved in the transduction of extracellular signals to intracellular targets in all eukaryotes. Distinct MAPK pathways are regulated by different extracellular stimuli and are implicated in a wide variety of biological processes. In plants, there is evidence for MAPKs playing a role in the signaling of abiotic stresses, pathogens, plant hormones, and cell cycle cues. The large number and divergence of plant MAPKs indicates that this ancient mechanism of bioinformatics is extensively used in plants and may provide new molecular hands on old questions.
Author: Rony Seger Publisher: Humana Press ISBN: 9781607617945 Category : Science Languages : en Pages : 530
Book Description
Mitogen-activated protein (MAP) kinase (MAPK) cascades are key signaling components that govern essentially all cellular processes evoked by any type of stimulation, and it has been well established that the malfunctioning of these cascades leads to various diseases including cancer, autoimmunity, and diabetes. In MAP Kinase Signaling Protocols, Second Edition, expert researchers fully update the popular first edition the key techniques used in the study of MAPK signaling cascades in various cellular contexts. This thorough volume explores essential topics such as activation and function of components of the MAPK signaling cascades, the study of MAPK cascades as transmitters of membranal receptor signals, structure-function relationships of MAPKs, studies on the regulation of MAPK cascades, the use of lower organisms, animal models, and human genetics in the study of MAPKs, as well as the study of MAPKs in specific systems and diseases. Written in the highly successful Methods in Molecular BiologyTM series format, chapters includes introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, MAP Kinase Signaling Protocols, Second Edition aims to facilitate the study of MAPKs and allow for quicker progress in our knowledge of many vital cellular processes as well as devastating diseases.
Author: Alastair D. Reith Publisher: Springer Science & Business Media ISBN: 1592590594 Category : Science Languages : en Pages : 367
Book Description
As key components of many cell signaling pathways, protein kinases are implicated in a broad variety of diseases, including cancers and neurodegenerative conditions, and offer considerable potential as tractable targets for therapeutic intervention. In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and provide more experienced investigators many novel time-saving ploys. Emphasis is given to the critical technical steps that are often omitted from methods published in the primary literature. There are also tips on potential pitfalls and copious notes on how to adjust the protocols to work in related systems. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.
Author: Kaladhar D. S. V. G. K. Publisher: LAP Lambert Academic Publishing ISBN: 9783659129193 Category : Languages : de Pages : 100
Book Description
Mitogen-activated protein (MAP) kinases (EC 2.7.11.24) are activated within the protein kinase cascades called "MAPK cascade." MAPK pathway contains a chain of proteins in the cell, which sends extracellular stimuli such as mitogens, osmotic stress, heat shock and proinflammatory cytokines, to the DNA in the nucleus. MAPK pathway is the best characterized signal transduction pathway in cell biology, plays a critical role in the infection-induced expression and in cancer. MAP kinase signal transduction system is essential for the rational design of novel pharmacotherapeutic approaches and in pathophysiology studies. The book provides basic idea in modulation of cellular activities such as Apoptosis, Cell Proliferation, Angiogenesis, Cancer, MAPK/ERK Pathway and Anti-Apoptosis. In silico application such as MAPK pathway analysis, molecular studies and docking techniques has been provided in the last chapter.
Author: Dafna Bar-Sagi Publisher: Springer Science & Business Media ISBN: 0896034887 Category : Cell membranes Languages : en Pages : 631
Book Description
This collection of practical, cutting-edge techniques for the study of cell signaling provides detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the newest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. Transmembrane Signaling Protocols makes available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling.
Author: Kandarp Harshadray Shah Publisher: ISBN: 9781124312217 Category : Languages : en Pages : 150
Book Description
Mitogen-activated protein kinase (MAPK) cascades in eukaryotic cells act in multiple pathways that share signaling components. Saccharomyces cerevisiae, the budding yeast, is an ideal model to study MAPK signaling specificity because its mating and invasive growth pathways share an entire cascade yet maintain specificity. The cascade contains the MAPK kinase kinase Ste11, MAPK kinase Ste7 and the MAPK Kss1. In addition there is another MAPK Fus3 that acts in mating. It has been proposed that Ste5, the scaffold for MAPK cascade, and Ste7 docking sites for Fus3 and Kss1 may promote specificity. Here the role of Ste7 docking sites in combination with Ste5 scaffolding of Ste7 was analyzed to understand signal transduction in both pathways. Results indicate that the two previously identified docking sites on Ste7 function in signal transduction for both mating and invasive growth. Fus3 can be phosphorylated in the absence of docking sites but requires the sites for full activation. Kss1 is entirely dependent on docking sites for its activation. In contrast, Ste5 scaffolding of Ste7 is required for mating but not for invasive growth. In absence of scaffolding, phosphorylation of Fus3 is reduced dramatically and level of Kss1 activation is reduced moderately. Interestingly, this moderate Kss1 activation by mating pheromone leads to a low-level induction of invasive growth genes but not mating genes, indicating that mating genes have a higher threshold for activation by Kss1. The low-level induction suggests that amount of active Kss1 is important for invasive growth and further indicates that Fus3-mediated negative feedback that controls Kss1 activation is critical in promoting mating specificity. The conclusion from these results is that Ste5 contributes to mating and invasive growth but it is only crucial for mating. The docking sites on Ste7 are crucial for both mating and invasive growth.
Author: Francesc Posas Publisher: Springer Science & Business Media ISBN: 3540755691 Category : Science Languages : en Pages : 322
Book Description
In this book leading researchers in the field discuss the state-of-the-art of many aspects of SAPK signaling in various systems from yeast to mammals. These include various chapters on regulatory mechanisms as well as the contribution of the SAPK signaling pathways to processes such as gene expression, metabolism, cell cycle regulation, immune responses and tumorigenesis. Written by international experts, the book will appeal to cell biologists and biochemists.
Author: Rony Seger
Author: Rony Seger
Author: George Komis
Author: Heribert Hirt
Author: Rony Seger
Author: Anisa Scott
Author: Alastair D. Reith
Author: Kaladhar D. S. V. G. K.
Author: Dafna Bar-Sagi
Author: Kandarp Harshadray Shah
Author: Francesc Posas