Integration of High Voltage CMOS and Microfluidic Technologies for Genetic Analysis Systems PDF Download

Author: Mohammad Behnam Dehkordi
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 244

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Author: Eric Lagally
Publisher: CRC Press
ISBN: 1351831488
Category : Medical
Languages : en
Pages : 294

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Book Description
An increasing number of technologies are being used to detect minute quantities of biomolecules and cells. However, it can be difficult to determine which technologies show the most promise for high-sensitivity and low-limit detection in different applications. Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit details proven approaches for the detection of single cells and even single molecules—approaches employed by the world’s foremost microfluidics and nanotechnology laboratories. While similar books concentrate only on microfluidics or nanotechnology, this book focuses on the combination of soft materials (elastomers and other polymers) with hard materials (semiconductors, metals, and glass) to form integrated detection systems for biological and chemical targets. It explores physical and chemical—as well as contact and noncontact—detection methods, using case studies to demonstrate system capabilities. Presenting a snapshot of the current state of the art, the text: Explains the theory behind different detection techniques, from mechanical resonators for detecting cell density to fiber-optic methods for detecting DNA hybridization, and beyond Examines microfluidic advances, including droplet microfluidics, digital microfluidics for manipulating droplets on the microscale, and more Highlights an array of technologies to allow for a comparison of the fundamental advantages and challenges of each, as well as an appreciation of the power of leveraging scalability and integration to achieve sensitivity at low cost Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit not only serves as a quick reference for the latest achievements in biochemical detection at the single-cell and single-molecule levels, but also provides researchers with inspiration for further innovation and expansion of the field.

Author: Samantha Anne Cronier
Publisher:
ISBN:
Category :
Languages : en
Pages : 110

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Book Description
Recent work in the areas of microfluidic technology and innovative new chemistries have made possible tremendous improvements in our ability to determine the nucleotide sequence of DNA. These advances are already changing the way doctors diagnose and treat human disease and enabling scientists to undertake genetic studies never before possible. In this dissertation I provide a brief history of DNA sequencing methods as well as a general description of microfluidic technologies, in particular those used for genetic analysis. Next, I describe my dissertation research on the development of a highly efficient fully integrated microfluidic platform for Sanger DNA sequencing, including automated thermal cycling, purification, concentration and in-line injection of the extension fragments for microchip capillary electrophoresis separation. The two-layer glass device that I developed features two independently operated valve-free systems, comprised of a 200 nL thermal cycling reactor with resistive temperature detector, a 1.2 nL in situ photopolymerized oligonucleotide affinity capture gel for post reaction clean-up and inline injection, and an 18-cm long capillary electrophoresis channel for separation. Integration of the efficient photopolymerized affinity gel capture allows sequencing from only 350 - 500 attomoles of starting DNA template. Using this device, I was able to sequence 507 ± 31 bases at 99% accuracy. In addition, I show that this method is compatible with single cell genetic analysis techniques (SCGA) by sequencing from the small amounts (1̃00 attomoles) of amplified DNA bound to agarose microbeads that can be produced from single cells. This dissertation concludes with a discussion of the future of DNA sequencing and the feasibility of performing single cell DNA sequencing using the Microbead Integrated DNA Sequencing (MINDS) method.

Author: Steffen Hardt
Publisher: Springer Science & Business Media
ISBN: 0387684247
Category : Technology & Engineering
Languages : en
Pages : 630

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Book Description
This book addresses Lab-on-a-Chip devices. It focuses on microfluidic technologies that have emerged in the past decade. Coverage presents a comprehensive listing of the most promising microfluidic technologies in the Lab-on-a-Chip field. It also details technologies that can be viewed as toolboxes needed to set up complex Lab-on-a-Chip systems.

Author: Yu Jiang
Publisher:
ISBN: 9781119218333
Category : HEALTH & FITNESS
Languages : en
Pages :

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Author: Lawrence Kwan Yeung Hui
Publisher:
ISBN:
Category :
Languages : en
Pages : 60

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Book Description
Over the past decade, interest in microfluidics has surged as applications have trended towards novel biological assays. Specifically, the ability of microfluidics to parallelize cellular studies through array-based chip designs has attracted researchers interested in investigating cellular function under a wide variety of environmental conditions. The capability of microfluidic devices to control microenvironment conditions and induce dynamic perturbation to cellular systems makes microfluidics (or "lab-on-a-chip") an attractive platform to study gene expression dynamics. In this project, the functionality of microfluidic technology is exploited to design and construct a device for isolation and observation of cells in high throughput. The integration of a concentration gradient with homogenous medium within each chamber was designed specifically to investigate gene regulation in Saccharomyces cerevisiae under various concentrations of chemical inducers. These devices were designed to sustain cells for extended periods of time with high temporal resolution to study dynamic gene expression in single cells. The device builds on previous studies by probing up to eight distinct cell cultures in parallel. The microfluidic platform was then used to study yeast cells at various levels of inducer perturbations. Further experimentation revealed the utility of a parallel gradient by producing an induction curve of the yeast response. Such high-throughput designs will prove essential to yeast systems biology research as it strives to understand the complex regulatory interactions that dictate cell function by probing vast regions of parameter space.

Author: Sushanta K. Mitra
Publisher: CRC Press
ISBN: 1439816735
Category : Science
Languages : en
Pages : 642

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Book Description
This comprehensive handbook presents fundamental aspects, fabrication techniques, introductory materials on microbiology and chemistry, measurement techniques, and applications of microfluidics and nanofluidics. The second volume focuses on topics related to experimental and numerical methods. It also covers fabrication and applications in a variety of areas, from aerospace to biological systems. Reflecting the inherent nature of microfluidics and nanofluidics, the book includes as much interdisciplinary knowledge as possible. It provides the fundamental science background for newcomers and advanced techniques and concepts for experienced researchers and professionals.

Author: Mohammad Behnam Dehkordi
Publisher:
ISBN:
Category : Biochips
Languages : en
Pages :

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Book Description

Author: Sushanta K. Mitra
Publisher: CRC Press
ISBN: 1466515740
Category : Science
Languages : en
Pages : 1767

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Book Description
A comprehensive, two-volume handbook on Microfluidics and Nanofluidics, this text covers fundamental aspects, fabrication techniques, introductory materials on microbiology and chemistry, measurement techniques, and applications with special emphasis on the energy sector. Each chapter begins with introductory coverage to a subject and then narrows in on advanced techniques and concepts, thus making it valuable to students and practitioners. The author pays special attention to applications of microfluidics in the energy sector and provides insight into the world of opportunities nanotechnology has to offer. Figures, tables, and equations to illustrate concepts.

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309111838
Category : Technology & Engineering
Languages : en
Pages : 38

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Book Description
The report on the EEEL presents an assessment of the Lab's four divisions. The assessment is based on four criteria: alignment with national priorities, motivation of its programs, technical merit, and technical program quality. The report also provides a look at three additional concerns: staffing and funding, international issues, and the planning process.