Author: R Dinakaran Michael
Publisher: Notion Press, Incorporated
ISBN: 9781644296332
Category :
Languages : en
Pages : 138
Book Description
Hello there, immunology teachers of colleges and universities! Are you still struggling to design and develop laboratory exercises to demonstrate concepts of immunology to your undergrad and postgrad students using rabbits, guinea pigs and mice without a good animal house? All your problems are over. Here is an innovative and 'easy to follow' laboratory manual which has the procedures of many immunological techniques and assays using fish model. Using fish model is mainly to avoid the constraints of having and maintaining spacious air-conditioned animal house and animals like rabbits, guinea pigs and mice which are traditionally used for immunology laboratory exercises. Fishes have most of the immunological components, mechanisms and molecules found in higher vertebrates like rabbits, guinea pigs and mice though in a simpler style. Hence, fish is as an ideal vertebrate model for teaching and demonstrating the essential immunological concepts. The immunological exercises covered in this manual includes but not restricted to the basic techniques like dissection of lymphoid organs, preparation of leucocytes from these organs, antigen preparation, immunization and bleeding technique, and serum separation and assays like non-specific immune mechanisms like lysozyme activity and production of reactive oxygen by phagocytes, specific immune responses like antibody production, allograft rejection and DTH reaction, expression of immune related genes and vaccination and vaccine efficacy testing. Many of these techniques are being routinely used in institutions where the author served and also in institutions whose faculty fellows underwent training in the large number of training workshops conducted by the author.
Immunological Techniques Using Fish Model - A Laboratory Manual
Author: R Dinakaran Michael
Publisher: Notion Press, Incorporated
ISBN: 9781644296332
Category :
Languages : en
Pages : 138
Book Description
Hello there, immunology teachers of colleges and universities! Are you still struggling to design and develop laboratory exercises to demonstrate concepts of immunology to your undergrad and postgrad students using rabbits, guinea pigs and mice without a good animal house? All your problems are over. Here is an innovative and 'easy to follow' laboratory manual which has the procedures of many immunological techniques and assays using fish model. Using fish model is mainly to avoid the constraints of having and maintaining spacious air-conditioned animal house and animals like rabbits, guinea pigs and mice which are traditionally used for immunology laboratory exercises. Fishes have most of the immunological components, mechanisms and molecules found in higher vertebrates like rabbits, guinea pigs and mice though in a simpler style. Hence, fish is as an ideal vertebrate model for teaching and demonstrating the essential immunological concepts. The immunological exercises covered in this manual includes but not restricted to the basic techniques like dissection of lymphoid organs, preparation of leucocytes from these organs, antigen preparation, immunization and bleeding technique, and serum separation and assays like non-specific immune mechanisms like lysozyme activity and production of reactive oxygen by phagocytes, specific immune responses like antibody production, allograft rejection and DTH reaction, expression of immune related genes and vaccination and vaccine efficacy testing. Many of these techniques are being routinely used in institutions where the author served and also in institutions whose faculty fellows underwent training in the large number of training workshops conducted by the author.
Publisher: Notion Press, Incorporated
ISBN: 9781644296332
Category :
Languages : en
Pages : 138
Book Description
Hello there, immunology teachers of colleges and universities! Are you still struggling to design and develop laboratory exercises to demonstrate concepts of immunology to your undergrad and postgrad students using rabbits, guinea pigs and mice without a good animal house? All your problems are over. Here is an innovative and 'easy to follow' laboratory manual which has the procedures of many immunological techniques and assays using fish model. Using fish model is mainly to avoid the constraints of having and maintaining spacious air-conditioned animal house and animals like rabbits, guinea pigs and mice which are traditionally used for immunology laboratory exercises. Fishes have most of the immunological components, mechanisms and molecules found in higher vertebrates like rabbits, guinea pigs and mice though in a simpler style. Hence, fish is as an ideal vertebrate model for teaching and demonstrating the essential immunological concepts. The immunological exercises covered in this manual includes but not restricted to the basic techniques like dissection of lymphoid organs, preparation of leucocytes from these organs, antigen preparation, immunization and bleeding technique, and serum separation and assays like non-specific immune mechanisms like lysozyme activity and production of reactive oxygen by phagocytes, specific immune responses like antibody production, allograft rejection and DTH reaction, expression of immune related genes and vaccination and vaccine efficacy testing. Many of these techniques are being routinely used in institutions where the author served and also in institutions whose faculty fellows underwent training in the large number of training workshops conducted by the author.
Manual of Immunological Methods
Author: Canadian Networking
Publisher: CRC Press
ISBN: 0429529031
Category : Medical
Languages : en
Pages : 164
Book Description
The Manual of Immunological Methods represents the collaboration of the Canadian Network of Toxicology Centers, a non-profit network of university-based scientists dedicated to research, training, risk assessment, and communication. This manual provides detailed immunological methods that can be utilized by researchers or practitioners who want to
Publisher: CRC Press
ISBN: 0429529031
Category : Medical
Languages : en
Pages : 164
Book Description
The Manual of Immunological Methods represents the collaboration of the Canadian Network of Toxicology Centers, a non-profit network of university-based scientists dedicated to research, training, risk assessment, and communication. This manual provides detailed immunological methods that can be utilized by researchers or practitioners who want to
Techniques in Fish Immunology
Techniques in Fish Immunology
Author: Joanne S. Stolen
Publisher: SOS Publications (NJ)
ISBN: 9780962550584
Category : Aquatic invertebrates
Languages : en
Pages : 0
Book Description
Publisher: SOS Publications (NJ)
ISBN: 9780962550584
Category : Aquatic invertebrates
Languages : en
Pages : 0
Book Description
Fish Immunology
Author: Douglas P. Anderson
Publisher: TFH Publications
ISBN:
Category : Medical
Languages : en
Pages : 276
Book Description
Publisher: TFH Publications
ISBN:
Category : Medical
Languages : en
Pages : 276
Book Description
Techniques in Insect Immunology
Author: Andreas Wiesner
Publisher: SOS Publications (NJ)
ISBN:
Category : Medical
Languages : en
Pages : 330
Book Description
The importance of insects for human welfare is unquestionable. This laboratory manual summarizes a number of key methods used by insect immunologists around the world. It should be helpful for those already working in this field & especially for scientists with general interest in this research topic as a methodical guide for the first steps. The experiments are described in detail, with step-by-step directions. Basic procedure for activation, detection, quantification & characterization of cellular & humoral defense reactions are given, along with protocols for more detailed investigations of cell differentiation & isolation of insect immune factors. Population genetic aspect, viral pathology & comparative studies revealing similarities with the vertebrate immune system are also included. The result described in the chapters show remarkable progress in our understanding of insect immunology. Simultaneously, the large number of still-unanswered questions make us realize that we have a lot left to learn about these fascinating animals. From future investigation we can expect a better understanding of the immune system's evolution, the discovery of new antimicrobial & regulatory insect molecules potentially useful in the therapy of human disease & new strategies for insect control.
Publisher: SOS Publications (NJ)
ISBN:
Category : Medical
Languages : en
Pages : 330
Book Description
The importance of insects for human welfare is unquestionable. This laboratory manual summarizes a number of key methods used by insect immunologists around the world. It should be helpful for those already working in this field & especially for scientists with general interest in this research topic as a methodical guide for the first steps. The experiments are described in detail, with step-by-step directions. Basic procedure for activation, detection, quantification & characterization of cellular & humoral defense reactions are given, along with protocols for more detailed investigations of cell differentiation & isolation of insect immune factors. Population genetic aspect, viral pathology & comparative studies revealing similarities with the vertebrate immune system are also included. The result described in the chapters show remarkable progress in our understanding of insect immunology. Simultaneously, the large number of still-unanswered questions make us realize that we have a lot left to learn about these fascinating animals. From future investigation we can expect a better understanding of the immune system's evolution, the discovery of new antimicrobial & regulatory insect molecules potentially useful in the therapy of human disease & new strategies for insect control.
Techniques in Fish Immunology
Author: Joanne S. Stolen
Publisher:
ISBN:
Category : Fishes
Languages : en
Pages : 246
Book Description
Publisher:
ISBN:
Category : Fishes
Languages : en
Pages : 246
Book Description
Forthcoming Books
Author: Rose Arny
Publisher:
ISBN:
Category : American literature
Languages : en
Pages : 1636
Book Description
Publisher:
ISBN:
Category : American literature
Languages : en
Pages : 1636
Book Description
Early Development of Xenopus Laevis
Author: Hazel L. Sive
Publisher: CSHL Press
ISBN: 9780879695040
Category : Medical
Languages : en
Pages : 370
Book Description
Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.
Publisher: CSHL Press
ISBN: 9780879695040
Category : Medical
Languages : en
Pages : 370
Book Description
Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.
Manual of Molecular and Clinical Laboratory Immunology
Author: Barbara Detrick
Publisher: John Wiley & Sons
ISBN: 1555818722
Category : Medical
Languages : en
Pages : 1240
Book Description
THE authoritative guide for clinical laboratory immunology For over 40 years the Manual of Molecular and Clinical Laboratory Immunology has served as the premier guide for the clinical immunology laboratory. From basic serology testing to the present wide range of molecular analyses, the Manual has reflected the exponential growth in the field of immunology over the past decades. This eighth edition reflects the latest advances and developments in the diagnosis and treatment of patients with infectious and immune-mediated disorders. The Manual features detailed descriptions of general and specific methodologies, placing special focus on the interpretation of laboratory findings, and covers the immunology of infectious diseases, including specific pathogens, as well as the full range of autoimmune and immunodeficiency diseases, cancer, and transplantation. Written to guide the laboratory director, the Manual will also appeal to other laboratory scientists, especially those working in clinical immunology laboratories, and pathologists. It is also a useful reference for physicians, mid-level providers, medical students, and allied health students with an interest in the role that immunology plays in the clinical laboratory.
Publisher: John Wiley & Sons
ISBN: 1555818722
Category : Medical
Languages : en
Pages : 1240
Book Description
THE authoritative guide for clinical laboratory immunology For over 40 years the Manual of Molecular and Clinical Laboratory Immunology has served as the premier guide for the clinical immunology laboratory. From basic serology testing to the present wide range of molecular analyses, the Manual has reflected the exponential growth in the field of immunology over the past decades. This eighth edition reflects the latest advances and developments in the diagnosis and treatment of patients with infectious and immune-mediated disorders. The Manual features detailed descriptions of general and specific methodologies, placing special focus on the interpretation of laboratory findings, and covers the immunology of infectious diseases, including specific pathogens, as well as the full range of autoimmune and immunodeficiency diseases, cancer, and transplantation. Written to guide the laboratory director, the Manual will also appeal to other laboratory scientists, especially those working in clinical immunology laboratories, and pathologists. It is also a useful reference for physicians, mid-level providers, medical students, and allied health students with an interest in the role that immunology plays in the clinical laboratory.