Glycopolymer Polyelectrolyte Multilayers Based on Maltose-modified Hyperbranched Poly(ethyleneimine) for Future Drug Delivery Coatings and Biomedical Applications PDF Download

Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 254

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Publisher: Scholar Press
ISBN: 6202306238
Category :
Languages : en
Pages : 268

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The theoretical background of this work is concerning with the drug loaded polyelectrolyte multilayers (PEM) modified by the host-guest interaction of biocompatible hyperbranched core-shell glycopolymers. The glycopolymer in this work is the hyperbranched polyethyleneimine that was modified with maltose moieties using reductive amination. Thus, the use of glycohyperbranched polymers for drug delivery would allow the avoid naturally occurring drug resistance due to decreased transporter activity. Concerning preparative method, PEM was fabricated using layer-by-layer (LbL) processes involve the sequential deposition of two polyions that physically bond together. Control was taken on the stoichiometric ratio related to cationic and anionic repeating units, which was chosen close to zero for the final applied PEM. Concerning analytical methods, a couple of physical-chemical methods were applied to characterize colloid stability, adhesiveness, drug loading and release of fabricated PEM. In conclusion, a highly sable and sustainable PEM coats on a surface of an activated solid substrate has been fabricated with an efficient ability to recycle the charged molecule for more than 24 times.

Author: Helen F. Chuang
Publisher:
ISBN:
Category :
Languages : en
Pages : 373

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Polyelectrolyte multilayers (PEMs) were fabricated via the layer-by-layer (LbL) deposition process, incorporating hydrolytically degradable poly([beta]-amino esters) to result in biodegradable PEMs that can release active ingredients in a dosage- and rate-tunable fashion. Specifically, PEMs incorporating several types of antibiotics, ranging from aminoglycosides to antimicrobial peptides (AmPs), were fabricated and characterized; these coatings are intended for applications onto biomedical device surfaces for infection control. In vitro efficacy against Staphylococcus aureus and nontoxicity towards preosteoblasts MC3T3 were demonstrated. In vivo evaluations involving a rabbit osteomyelitis model were undertaken as well. Aside from the development of antimicrobial PEMs, additional projects pursued under this thesis, all in the context of PEM-based drug delivery, include [1] demonstration of the sequential release of two species, [2] assessment of the in vitro activity of anticoagulant films, [3] delivery of siRNAs, [4] evaluation of the biocompatibility of poly([beta]- amino esters), [5] incorporation of cyclodextrins for the purpose of small molecule delivery, [6] incorporation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating gentamicin, [7] evaluation of film sterilizability via FDA-approved methods, and [8] design and characterization of a multi-drug coating for orthopedic implants for dual antimicrobial and tissue regenerative actions.

Author: Sukant K. Tripathy
Publisher:
ISBN:
Category : Polyelectrolytes
Languages : en
Pages : 378

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Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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The work described in this thesis focuses on the evaluation of synthetic organic materials to either (i) mediate the delivery of DNA from thin polymer films on surfaces or (ii) create functional coatings on surfaces with potential biomedical applications related to drug delivery and cell adhesion. In both cases, layer-by-layer fabrication was used to assemble films on surfaces through the iterative immersion of substrates in alternating solutions of complementary functionality (e.g. alternating solutions of polycations and polyanions or alternating solutions of organic materials possessing nucleophilic or electrophilic functional groups in order to create polyelectrolyte and covalent multilayers, respectively). The DNA-related studies build upon previous work demonstrating that films fabricated using a hydrolytically degradable cationic polymer can release DNA over a period of one to two days and promote transgene expression both in vitro and in vivo. Chapter 2 reports the synthesis of a fluorescently end-labeled degradable cationic polymer and its application to characterize the erosion of polyelectrolyte multilayers fabricated using a model polyanion. Chapter 3 used this fluorescently labeled cationic polymer to provide more direct insight into fundamental interactions between the polymer and DNA during release from films and during trafficking by cells in vitro. The results of this chapter suggest that the polymer components of these films can play a role in promoting cellular uptake and trafficking of DNA. Chapter 4 characterized the application of this DNA-containing film system to deliver DNA encoding a potential therapeutic protein to investigate the extent to which this method of delivery could be used to reduce intimal hyperplasia in arterial tissue of rats after balloon-mediated injury. The results of this study provide guidance for the development of degradable polyelectrolyte multilayered thin films for the surface-mediated delivery of DNA. Chapter 5 demonstrated layer-by-layer fabrication of covalent films using a branched polyamine and small molecule pentacrylate to yield films possessing hydrolytically degradable ester bonds. These films were subsequently functionalized using unreacted acrylate groups to control film hydrophobicity and cell adhesion and provided a platform for the release of covalently attached molecules upon incubation in aqueous media.

Author: Xi Zhang
Publisher: Royal Society of Chemistry
ISBN: 1782625429
Category : Science
Languages : en
Pages : 183

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An amphiphile is a molecule that contains a hydrophilic part and a hydrophobic part, linked by covalent bonding. Supramolecular amphiphiles (supra-amphiphiles) are amphiphiles linked by non-covalent interactions. As they employ non-covalent interactions, these species demonstrate adaptability and reversibility in conformational transformation, making them one of the most important emerging species in supramolecular chemistry. They have proven important in bridging the gap between molecular architecture and functional assembly. This book is written and edited by the current leaders in the topic and contains a foreword from Professor Jean-Marie Lehn, a father of the supramolecular chemistry field. Bringing together supramolecular chemistry and colloidal and interfacial science, the book provides a detailed and systematic introduction to supramolecular amphiphiles. Chapters explain how to employ non-covalent interactions to fabricate supra-amphiphiles. The book opens with an introduction to the history and development of the field, followed by chapters focussing on each type of interaction, including host-guest interaction, electrostatic interaction, charge-transfer interaction, hydrogen bonding and dynamic covalent bonds. This book will be a valuable resource for students new to this field and experienced researchers wanting to explore the wider context of their work.

Author: Ephraim Cohen
Publisher: Springer
ISBN: 3030129195
Category : Science
Languages : en
Pages : 820

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The extracellular matrix (ECM) is an acellular three-dimensional network composed of proteins, glycoproteins, proteoglycans and exopolysaccharides. It primarily serves as a structural component in the tissues and organs of plants and animals, or forms biofilms in which bacterial cells are embedded. ECMs are highly dynamic structures that undergo continuous remodeling, and disruptions are frequently the result of pathological processes associated with severe diseases such as arteriosclerosis, neurodegenerative illness or cancer. In turn, bacterial biofilms are a source of concern for human health, as they are associated with resistance to antibiotics. Although exopolysaccharides are crucial for ECM formation and function, they have received considerably little attention to date. The respective chapters of this book comprehensively address such issues, and provide reviews on the structural, biochemical, molecular and biophysical properties of exopolysaccharides. These components are abundantly produced by virtually all taxa including bacteria, algae, plants, fungi, invertebrates and vertebrates. They include long unbranched homopolymers (cellulose, chitin/chitosan), linear copolymers (alginate, agarose), peptoglycans such as murein, heteropolymers like a variety of glycosaminoglycans (hyaluronan, dermatan, keratin, heparin, Pel), and branched heteropolymers such as pectin and hemicellulose. A separate chapter is dedicated to modern industrial and biomedical applications of exopolysaccharides and polysaccharide-based biocomposites. Their unique chemical, physical and mechanical properties have attracted considerable interest, inspired basic and applied research, and have already been harnessed to form structural biocomposite hybrids for tailor-made applications in regenerative medicine, bioengineering and biosensor design. Given its scope, this book provides a substantial source of basic and applied information for a wide range of scientists, as well as valuable textbook for graduate and advanced undergraduate students.

Author: Robert Tattersall
Publisher:
ISBN:
Category : Health & Fitness
Languages : en
Pages : 404

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Author: Timothy Deming
Publisher: Springer Science & Business Media
ISBN: 3642271391
Category : Technology & Engineering
Languages : en
Pages : 184

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Synthesis of Polypeptides by Ring-Opening Polymerization of α-Amino Acid N-Carboxyanhydrides, by Jianjun Cheng and Timothy J. Deming.- Peptide Synthesis and Self-Assembly, by S. Maude, L. R. Tai, R. P. W. Davies, B. Liu, S. A. Harris, P. J. Kocienski and A. Aggeli.- Elastomeric Polypeptides, by Mark B. van Eldijk, Christopher L. McGann, Kristi L. Kiick andJan C. M. van Hest.- Self-Assembled Polypeptide and Polypeptide Hybrid Vesicles: From Synthesis to Application, by Uh-Joo Choe, Victor Z. Sun, James-Kevin Y. Tan and Daniel T. Kamei.- Peptide-Based and Polypeptide-Based Hydrogels for Drug Delivery and Tissue Engineering, by Aysegul Altunbas and Darrin J. Pochan.-

Author: C. Remzi Becer
Publisher: Royal Society of Chemistry
ISBN: 1782624686
Category : Science
Languages : en
Pages : 251

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Glycopolymers are important for cell signalling, recognition pathways, and their role in the immune system. They are gaining attention for new applications in tissue engineering and drug delivery. Glycopolymer Code discusses the preparation, characterization and applications of glycopolymers providing a complete overview of the topic with examples from the latest research. Specific chapters cover both basic and advanced synthesis techniques to prepare glycopolymers, the analytical techniques used to investigate lectin receptor glycopolymer interactions, the properties and types of lectins that are widely used to understand the multivalent interactions and various applications of synthetic glycopolymers. With contributions from leading researchers in the field, the book is a unique source for graduates and academics new to the subject and already working in the area of glycopolymers.