Gel Electrophoresis and Isoelectric Focusing of Proteins PDF Download

Author: Robert C. Allen
Publisher: Walter de Gruyter GmbH & Co KG
ISBN: 3110863634
Category : Science
Languages : en
Pages : 280

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Book Description
No detailed description available for "Gel Electrophoresis and Isoelectric Focusing of Proteins".

Author: P. G. Righetti
Publisher: Elsevier
ISBN: 0444813012
Category : Science
Languages : en
Pages : 415

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Book Description
Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.

Author: Reiner Westermeier
Publisher: John Wiley & Sons
ISBN: 3527338802
Category : Science
Languages : en
Pages : 470

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Book Description
"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.

Author: Pawel Ciborowski
Publisher: Elsevier
ISBN: 0444636900
Category : Science
Languages : en
Pages : 300

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Book Description
Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. - Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure - Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers - Helps in designing and executing proteomic experiments with sound analytics

Author: Biji T. Kurien
Publisher:
ISBN: 9781493987931
Category : Electrophoresis
Languages : en
Pages : 523

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Author: Pier Giorgio Righetti
Publisher: Elsevier
ISBN: 0080858805
Category : Science
Languages : en
Pages : 403

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Book Description
This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.

Author: Oscar Alzate
Publisher: CRC Press
ISBN: 1420076264
Category : Medical
Languages : en
Pages : 356

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Book Description
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg

Author: T. Rabilloud
Publisher: Springer Science & Business Media
ISBN: 9783540657927
Category : Science
Languages : en
Pages : 272

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Book Description
With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.

Author: Ashok Pandey
Publisher: Elsevier
ISBN: 0444636730
Category : Science
Languages : en
Pages : 888

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Book Description
Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, focusing on industrial biotechnology and bioengineering practices for the production of industrial products, such as enzymes, organic acids, biopolymers, and biosurfactants, and the processes for isolating and purifying them from a production medium. During the last few years, the tools of molecular biology and genetic and metabolic engineering have rendered tremendous improvements in the production of industrial products by fermentation. Structured by industrial product classifications, this book provides an overview of the current practice, status, and future potential for the production of these agents, along with reviews of the industrial scenario relating to their production. - Provides information on industrial bioprocesses for the production of microbial products by fermentation - Includes separation and purification processes of fermentation products - Presents economic and feasibility assessments of the various processes and their scaling up - Links biotechnology and bioengineering for industrial process development

Author: John M. Walker
Publisher: Springer Science & Business Media
ISBN: 0896039404
Category : Medical
Languages : en
Pages : 1446

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Book Description
The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.