Author: Wouterus Thomas Maria GruijtersPublisher:
ISBN:
Category : Cell membranes
Languages : en
Pages : 84
Book Description
Freeze-fracture Studies on Yeast Plasma Membranes
Author: Wouterus Thomas Maria GruijtersPublisher:
ISBN:
Category : Cell membranes
Languages : en
Pages : 84
Book Description
Freeze-Fracture Studies of Membranes
Author: Sek Wen HuiPublisher: CRC Press
ISBN: 1351080601
Category : Medical
Languages : en
Pages : 229
Book Description
This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.
Freeze-fracture Study of the Plasma Membrane of Schizophyllum Commune
Author: Susan Baker WardePublisher:
ISBN:
Category : Cell membranes
Languages : en
Pages : 120
Book Description
Structural and Physiological Studies on the Crystalline Membrane Pariticles in the Yeast Saccharomyces Cerevisiae
Author: Gina Ellen SosinskyRapid Freezing, Freeze Fracture, and Deep Etching
Author: Nicholas J. SeversPublisher: Wiley-Liss
ISBN:
Category : Medical
Languages : en
Pages : 392
Book Description
The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell. Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze fracture. Advanced techniques are presented next, with each chapter devoted to an in-depth treatment of a specific topic, including the advantages and limitations of each technique. The final chapters offer a series of applications selected to illustrate the wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical research. With over 150 illustrations and containing contributions by leading experts instrumental in the development of these techniques, Rapid Freezing, Freeze Fracture, and Deep Etching offers authoritative coverage of such novel topics as: Hyperbaric freezing Ultrarapid freezing techniques in the study of dynamic cellular processes Cytochemical methods utilizing lectins and antibodies for labeling specific cellcomponents Fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions Freeze fracture and deep etching in neurobiology Rapid Freezing, Freeze Fracture, and Deep Etching will appeal to both newcomers to the field and experienced researchers and will be essential reading for cell biologists and anatomists, as well as any researcher who uses electron microscopy.
Molecular Biology of the Cell
Author: Effects of Freezing and Cold Acclimation on the Plasma Membrane of Isolated Protoplasts
Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 7
Book Description
Our goal is to provide a mechanistic understanding of the cellular and molecular aspects of freezing injury and cold acclimation from a perspective of the structural and functional integrity of the plasma membrane -- the primary site of freezing injury in winter cereals. We have utilized protoplasts isolated from leaves of winter rye (Secale cereale L. cv Puma) to study the cryobehavior of the plasma membrane during a freeze/thaw cycle. The focus of our current studies is on lesions in the plasma membrane that result from severe freeze-induced dehydration and result in the alteration of the semipermeable characteristics of the plasma membrane so that the protoplasts are osmotically unresponsive. In protoplasts isolated from non-acclimated rye leaves (NA protoplasts), injury is associated with the formation of aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and lamellar-to-hexagonal II phase transitions in the plasma membrane and the subtending lamellae. However, lamellar-to-hexagonal II phase transitions are not observed following severe dehydration of protoplasts isolated from cold-acclimated rye leaves (ACC protoplasts). Rather, injury is associated with the fracture-jump lesion, '' which, in freeze-fracture electron microscopy studies, is manifested as localized deviations in the fracture face of the plasma membrane. The fracture plane jumps'' from the plasma membrane to either subtending aparticulate lamellae or aparticulate regions of various endomembranes (predominantly chloroplast envelopes) that are in close apposition with the plasma membrane.
Effects of Freezing and Cold Acclimation on the Plasma Membrane of Isolated Protoplasts, Progress Report
Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 7
Book Description
Our goal is to provide a mechanistic understanding of the cellular and molecular aspects of freezing injury and cold acclimation from a perspective of the structural and functional integrity of the plasma membrane -- the primary site of freezing injury in winter cereals. We have utilized protoplasts isolated from leaves of winter rye (Secale cereale L. cv Puma) to study the cryobehavior of the plasma membrane during a freeze/thaw cycle. The focus of our current studies is on lesions in the plasma membrane that result from severe freeze-induced dehydration and result in the alteration of the semipermeable characteristics of the plasma membrane so that the protoplasts are osmotically unresponsive. In protoplasts isolated from non-acclimated rye leaves (NA protoplasts), injury is associated with the formation of aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and lamellar-to-hexagonal II phase transitions in the plasma membrane and the subtending lamellae. However, lamellar-to-hexagonal II phase transitions are not observed following severe dehydration of protoplasts isolated from cold-acclimated rye leaves (ACC protoplasts). Rather, injury is associated with the fracture-jump lesion, '' which, in freeze-fracture electron microscopy studies, is manifested as localized deviations in the fracture face of the plasma membrane. The fracture plane jumps'' from the plasma membrane to either subtending aparticulate lamellae or aparticulate regions of various endomembranes (predominantly chloroplast envelopes) that are in close apposition with the plasma membrane.
Biomembrane Structures
Author: Parvez I. HarisPublisher: IOS Press
ISBN: 9789051993233
Category : Medical
Languages : en
Pages : 288
Book Description
Biological membranes play a significant role in a range of biological processes such as ion-transport and signal transduction. Over the years much effort has been devoted towards developing an understanding of biomembrane structure. The study of this subject is now reaching an important stage. This is because at last the full three-dimensional structure of certain membrane proteins is beginning to be resolved. In the past three-dimensional structures of membrane proteins were difficult to obtain as only two dimensional crystals were available. In recent years satisfactory crystals have been obtained and X-ray diffraction techniques have been applied. This has led to the three dimensional structures of the photosynthetic reaction centres, porins and more recently the structure of cytochrome oxidase. Of course not all membrane proteins are readily crystallisable and some are not even available in sufficient quantities to obtain the necessary crystals or to carry out biophysical experiments. In some cases e.g. the voltage-gated potassium ion channel membrane proteins their structure has been proposed mainly on the basis of molecular biology methods. This has prompted the search for alternative approaches for characterising biomembrane structure. Molecular biological studies are providing a wealth of information on a number of different membrane proteins. Combining the information derived from such studies with molecular modelling is becoming extremely useful for relating structure to function. Development of other approaches include synthesis and structure- function analysis of peptides corresponding to functionally important domains of membrane proteins. This book presents a series of Chapters discussing how a combination of molecular biological, biophysical and theoretical (molecular modelling) techniques are helping us to obtain a much clearer picture of biomembrane structure. After an introductory Chapter on the Principles of membrane Protein Structure, the book is divided into two sections; one dealing with crystallographic approaches and the other non-crystallographic approaches such as NMR, AFM, SPR and FTIR spectroscopy. Chapters dealing with the recently solved crystal structure of cytochrome oxidase and bacteriorhodopsin are presented. The book contains contributions from leading membrane scientists describing their latest studies. It provides an up to date coverage of the developments in the field of biomembranes with particular emphasis on membrane proteins.
Effects of Freezing and Cold Acclimation on the Plasma Membrane of Isolated Protoplasts. Progress Report, May 16, 1992--January 9, 1993
Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 7
Book Description
Our goal is to provide a mechanistic understanding of the cellular and molecular aspects of freezing injury and cold acclimation from a perspective of the structural and functional integrity of the plasma membrane -- the primary site of freezing injury in winter cereals. We have utilized protoplasts isolated from leaves of winter rye (Secale cereale L. cv Puma) to study the cryobehavior of the plasma membrane during a freeze/thaw cycle. The focus of our current studies is on lesions in the plasma membrane that result from severe freeze-induced dehydration and result in the alteration of the semipermeable characteristics of the plasma membrane so that the protoplasts are osmotically unresponsive. In protoplasts isolated from non-acclimated rye leaves (NA protoplasts), injury is associated with the formation of aparticulate domains in the plasma membrane, aparticulate lamellae subtending the plasma membrane, and lamellar-to-hexagonal II phase transitions in the plasma membrane and the subtending lamellae. However, lamellar-to-hexagonal II phase transitions are not observed following severe dehydration of protoplasts isolated from cold-acclimated rye leaves (ACC protoplasts). Rather, injury is associated with the ''fracture-jump lesion, '' which, in freeze-fracture electron microscopy studies, is manifested as localized deviations in the fracture face of the plasma membrane. The fracture plane ''jumps'' from the plasma membrane to either subtending aparticulate lamellae or aparticulate regions of various endomembranes (predominantly chloroplast envelopes) that are in close apposition with the plasma membrane.