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Author: Sek Wen Hui Publisher: CRC Press ISBN: 1351080601 Category : Medical Languages : en Pages : 229
Book Description
This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.
Author: Sek Wen Hui Publisher: CRC Press ISBN: 1351080601 Category : Medical Languages : en Pages : 229
Book Description
This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The structure and organization of biological membranes were studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts have been probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and electron microscope autoradiographic techniques has been developed and tested and a new method of rotary shadow freeze-etching has been evaluated. The role of lipid-lipid and protein-protein interactions in controlling the mobility of cell surface macromolecules has been studied using freeze-fracture, protein extraction and SDS-polyacrylamide gel techniques.
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The researchers devised a rapid procedure for measuring covalent bond breakage in a simple model system to determine the extent to which glycophorin was cleaved when the frozen vesicles were fractured. Evidence showed that no covalent bonds are broken during the fracture procedure. Membrane splitting was shown to be quantified under special circumstances when erythrocyte membranes are appressed to a polylysine-coated surface. Results show that freeze fracture at temperatures below that of liquid nitrogen results in the appearance of considerable detail on E faces of purple membranes. Detail seems to improve as the temperature is lowered, suggesting that the smooth E faces in conventional freeze fractures may be a result of plastic deformation and flow of membrane components. Results show that ultra-low temperature freeze-fracturing can reduce plastic distortion and, particularly when used in conjunction with rotary replication, should improve our ability to discern small labels attached to transmembrane proteins. (PCS).
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The structure and organization of biological membranes is being studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts are being probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and biochemical techniques using fluorescent resonance energy transfer has been developed and tested and ultra low temperature freeze-etching has been evaluated as part of a program to develop covalent labelling techniques that will identify specific transmembrane proteins in freeze-fractured replicas.
Author: William Stillwell Publisher: Newnes ISBN: 0080931286 Category : Science Languages : en Pages : 379
Book Description
An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. - Brings together different facets of membrane research in a universally understandable manner - Emphasis on the historical development of the field - Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The structure and organization of biological membranes has been studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts have been probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and biochemical techniques using fluorescent resonance energy transfer has been developed and tested and ultra low temperature freeze-etching has been evaluated as part of a program to develop covalent labelling techniques that will identify specific transmembrane proteins in freeze-fractured replicas.
Author: Sek Wen Hui
Author: Sek Wen Hui
Author: Wolfgang Kleemann
Author: Wouterus Thomas Maria Gruijters
Author: John E. Rash
Author: ![Studies of Membrane Structure by Freeze-etching. Progress Report, 1 July 1975--30 June 1976. [Erythrocyte Membranes]. PDF](https://books.google.com/books/content/images/frontcover/9LjFwQEACAAJ?fife=w80-h100)
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Author: William Stillwell
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