Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates PDF Download

Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 0080489389
Category : Science
Languages : en
Pages : 551

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Book Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Spectroscopic Detection of Reaction Intermediates - Isotopic and Kenetic Detection of Reaction Intermediates - Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates

Author: Daniel L. Purich
Publisher: Academic Press
ISBN: 9780121822576
Category : Science
Languages : en
Pages : 0

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Book Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.

Author: Paul F. Cook
Publisher: Garland Science
ISBN: 1136844287
Category : Medical
Languages : en
Pages : 416

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Book Description
Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

Author: Daniel L. Purich
Publisher:
ISBN: 9780121822576
Category : Medical
Languages : en
Pages : 515

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Book Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Spectroscopic Detection of Reaction Intermediates * Isotopic and Kenetic Detection of Reaction Intermediates * Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates

Author:
Publisher: Academic Press
ISBN: 0080923364
Category : Science
Languages : en
Pages : 644

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Book Description
Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine. Gathers tried and tested methods and techniques from top players in the field Provides an extremely useful reference for the experienced research scientist Covers biosynthesis of Polyketides, Tarpenoids, Aminocoumarins and Crabohydrates

Author: Lynne E. Maquat
Publisher: Academic Press
ISBN: 0080922074
Category : Science
Languages : en
Pages : 661

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Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression - Offers step-by-step lab instructions, including necessary equipment and reagents

Author: Lynne E. Maquat
Publisher: Academic Press
ISBN: 0080923321
Category : Science
Languages : en
Pages : 463

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Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques - Offers step-by-step lab instructions, including necessary equipment and reagents

Author: Helmut Sies
Publisher: Elsevier
ISBN: 0080497160
Category : Science
Languages : en
Pages : 461

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Book Description
Quinones are members of a class of aromatic compounds with two oxygen atoms bonded to the ring as carbonyl groups. This volume covers the role of quinines enzymes in cellular signalling and modulation of gene expression.*Coenzyme Q: Detection and Quinone Reductases*Plasma Membrane Quinone Reductases*Quinones, Cellular Signaling, and Modulation of Gene Expression

Author: Carl Wu
Publisher: Elsevier
ISBN: 0080497136
Category : Science
Languages : en
Pages : 579

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Book Description
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription.*Histone Bioinformatics*Biochemistry of histones, nucleosomes and chromatin*Molecular cytology of chromatin functions

Author: Carl Wu
Publisher: Elsevier
ISBN: 0080497144
Category : Science
Languages : en
Pages : 497

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Book Description
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.