Electron Microscopic Mapping of the Second Polytene Chromosome of Drosophila Melanogaster PDF Download

Author: Anja O. Saura
Publisher:
ISBN:
Category :
Languages : en
Pages : 72

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Author: Gerard Morel
Publisher: CRC Press
ISBN: 9780849347818
Category : Science
Languages : en
Pages : 398

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This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.

Author: Daryl S. Henderson
Publisher: Springer Science & Business Media
ISBN: 1592596657
Category : Science
Languages : en
Pages : 467

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Book Description
Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Author:
Publisher: Academic Press
ISBN: 0080568211
Category : Science
Languages : en
Pages : 505

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This volume of Advances in Genetics illustrates the series goal to publish the most current, up-to-date reviews in the field of molecular and human genetics. This volume deviates from previous volumes in that it focuses solely on the polytene chromosome. In an excellent and exhaustive review, I.F. Zhimulev illustrates the use and function of the polytene chromosome in three parts: morphology and structure, organization and functional role of the heterochromaticregions, and the transcription patterns and replication of the chromosome. To best illustrate the breadth and scope of research, Dr. Zhimulev includes more than 100 figures and more than 2900 references specifically relevant to the polytene chromosome. - Presents technical and historical overviews of molecular biology applied to disease detection, diagnosis, and treatment - Chronicles the continuing explosion of knowledge in molecular genetic medicine by highlighting current approaches to understanding human illness - Documents the revolution in human and molecular genetics leading to a new field of medicine

Author: M. Ashburner
Publisher:
ISBN:
Category : Nature
Languages : en
Pages : 1380

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Author: Dan L. Lindsley
Publisher: Academic Press
ISBN: 0323139841
Category : Science
Languages : en
Pages : 1144

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Dedicated to the memory of George Lefevre in recognition of his exhaustive cytogenetic analysis of the X chromosome, The Genome of Drosophila melanogaster is the complete compendium of what is known about the genes and chromosomes of this widely used model organism. The volume is an up-to-date revision of Lindsley and Grell's 1968 work, Genetic Variations of Drosophila melanogaster. The new edition contains complete descriptions of normal and mutant genes including phenotypic, cytological, molecular, and bibliographic information. In addition, it describes thousands of recorded chromosome rearrangements used in research on Drosophila. This handbook and its accompanying polytene chromosome maps, are sturdily bound into the book as foldouts and available as a separate set, are essential research tools for the Drosophila community. - Describes phenotype, cytology, and molecular biology of all recorded genes of Drosophila melanogaster, plus references to the literature - Describes normal chromosome complement, special chromosome constructs, transposable elements, departures from diploidy, satellite sequences, and nonchromosomal inheritance - Describes all recorded chromosome rearrangements of Drosophila melanogaster as of the end of 1989 Contains the cytogenetic map of all genes as of mid-1991 - Contains the original polytene maps of C.B. Bridges, plus G. Lefevre's photographic equivalents, and the detailed maps of the chromosome arms produced by C.B. and P.M. Bridges - All maps are reprinted as high-quality foldouts sturdily bound into the volume - Maps may also be purchased separately in an eight-map packet, for laboratory and student use

Author: Veikko Sorsa
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 300

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Author: Kwang W. Jeon
Publisher: Academic Press
ISBN: 0123646456
Category : Medical
Languages : en
Pages : 333

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International Review of Cytology presents current advances and comprehensive reviews in cell biology - both plant and animal. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. Articles in this volume address biosynthesis and alternate targeting of the lysosomal cysteine protesase cathepsin L; microtubule-associated proteins and their essential roles during mitosis; molecular and functional analysis of the dictyostelium centrosome; polytene chromosomes; and insect basic leucine zipper proteins and their role in cyclic AMP dependent regulation of gene expression.

Author: H. Akai
Publisher: Springer Science & Business Media
ISBN: 1461327156
Category : Science
Languages : en
Pages : 637

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Book Description
Cell biology is moving at breakneck speed, and many of the results from studies on insects have helped in understanding some of the central problems of biology. The time is therefore ripe to provide the scientific community with a series of up-to-date, well illustrated reviews of selected aspects of the sub microscopic cytology of insects. The topics we have included fall into four general groups: seven chapters deal with gametogenesis, four concern develop ing somatic cells, seventeen chapters describe specialized tissues and organs, and three chapters cover cells in pathological states. These accounts are illustrated with over 600 electron micrographs. The more than 1100 pages in the two volumes of Insect Ultrastructure combined labors of 49 dedicated contributors from II countries. represent the These authors have digested and critically summarized a very large body of information, and some measure of this effort can be gained from consulting the bibliographies that close each of the 31 chapters. These contain 2400 publica tions authored by 1500 different scientists. However, before we congratulate ourselves on the advanced state of our knowledge, it is worth remembering that representatives of less than 0.01 % of the known species of insects have been examined with the electron microscope.

Author: Donald D. Brown
Publisher: Elsevier
ISBN: 0323138624
Category : Health & Fitness
Languages : en
Pages : 725

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Book Description
Developmental Biology Using Purified Genes is a compilation of papers presented at the 1981 ICN-UCLA Symposia on Developmental Biology Using Purified Genes, held in Keystone, Colorado. Contributors representing a wide range of disciplines explore the mechanisms underlying gene control of development and explain how purified genes are transcribed in cells, how DNA sequences and non-DNA molecules regulate development, and how gene-control molecules or other developmental determinants are unequally distributed among embryonic cells. Organized into nine sections comprised of 54 chapters, this volume begins with an overview of the mechanism by which gene activity is regionally controlled and its role in development. It then proceeds with a discussion on eukaryotic genes and their structure, including the collagen gene and the albumin gene family. The next chapters focus on the transcription and translation of yolk protein mRNA in the fat bodies of Drosophila, the organization and expression of the actin multi-gene family in Dictyostelium, the cDNA clones encoding mouse transplantation antigens, and the role of double minute chromosomes in unstable methotrexate resistance. The book also introduces the nucleosome core particle, regulatory factors involved in the transcription of mouse ribosomal genes, and developmental control of 5S RNA gene expression before concluding with a chapter on synthetic oligodeoxyribonucleotides and their use in the isolation of specific cloned DNA sequences. This book will be of interest to microbiologists, molecular biologists, embryologists, geneticists, and researchers working in the fields of genetics and developmental biology.