DISSOCIATION SUR SURFACE EN SPECTROMETRIE DE MASSE TANDEM REALISATION INSTRUMENTALE ET APPLICATIONS PDF Download

Author: THIERRY.. BLASCO
Publisher:
ISBN:
Category :
Languages : fr
Pages : 186

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LA SPECTROMETRIE DE MASSE TANDEM EST UNE METHODE MICROANALYTIQUE DESTRUCTRICE PERMETTANT EN PARTICULIER L'ANALYSE STRUCTURALE DE COMPOSES ORGANIQUES ET BIOLOGIQUES. LA METHODE CLASSIQUEMENT UTILISEE (CID) CONSISTE A SELECTIONNER UN FAISCEAU D'IONS AVEC UN PREMIER ANALYSEUR EN MASSE, A L'EXCITER EN LUI FAISANT SUBIR DES COLLISIONS AVEC DES ATOMES OU MOLECULES D'UN COMPOSE EN PHASE GAZEUSE, ET A ANALYSER LES IONS FRAGMENTS OBTENUS PAR DISSOCIATION DES IONS ACTIVES A L'AIDE D'UN DEUXIEME ANALYSEUR. UNE ALTERNATIVE, PROPOSEE EN 1985, CONSISTE A EXCITER LES IONS PAR COLLISION AVEC UNE SURFACE METALLIQUE (SID). L'APPLICATION DE CETTE METHODE A L'ETUDE DE LA FRAGMENTATION DE DIVERS TYPES DE COMPOSES (ALCANE, PEPTIDE, POLYAROMATIQUE HYDROCARBONE) MONTRE QU'ELLE PERMET UN MEILLEUR CONTROLE DE L'EXCITATION DE L'ION INCIDENT ET UN ACCES A DES ENERGIES D'ACTIVATION PLUS ELEVEES. ELLE PRESENTE TOUTEFOIS UN DESAVANTAGE MAJEUR: LA NEUTRALISATION PARTIELLE DU FAISCEAU INCIDENT, QUI CONDUIT A UNE PERTE DE SENSIBILITE. CETTE TECHNIQUE A ETE ETUDIEE SUR TROIS APPAREILS DIFFERENTS, EN ESSAYANT DIFFERENTES GEOMETRIES D'OPTIQUE IONIQUE ET DIFFERENTS TYPES DE SURFACE (SURFACE METALLIQUE EN ACIER INOXYDABLE, CUIVRE OU ALUMINIUM ET SURFACE SEMI-CONDUCTRICE CONSTITUEE D'UNE GALETTE DE MICROCANAUX). LES RESULTATS OBTENUS MONTRENT UNE NEUTRALISATION ELEVEE POUR LES SURFACES METALLIQUES, PLUS FAIBLE DANS LE CAS DE LA SURFACE EN ALUMINIUM ET ENCORE PLUS FAIBLE DANS CELUI DE LA GALETTE A MICROCANAUX. TOUTEFOIS, CETTE DERNIERE SOLUTION SEMBLE PEU ADAPTEE A LA GAMME D'ENERGIE CINETIQUE COMPATIBLE AVEC LES APPAREILS A FILTRE QUADRIPOLAIRE UTILISES (QUELQUES EV A 100EV), L'INCIDENCE RASANTE DU FAISCEAU LIMITANT L'ENERGIE D'ACTIVATION. L'ENSEMBLE DES RESULTATS OBTENUS PERMET D'ETABLIR QUE CETTE TECHNIQUE D'ACTIVATION PEUT PERMETTRE UNE EXTENSION DU DOMAINE D'APPLICATION DE LA SPECTROMETRIE DE MASSE TANDEM, EN PARTICULIER POUR L'ANALYSE STRUCTURALE DE MOLECULES DE GRANDE TAILLE. LA RECHERCHE DE MATERIAUX MINIMISANT LA NEUTRALISATION PARTIELLE SEMBLE TOUTEFOIS INDISPENSABLE

Author: BLASCO.
Publisher:
ISBN:
Category :
Languages : fr
Pages : 200

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Author: Raymond E. March
Publisher: Wiley-Interscience
ISBN: 9780471717980
Category : Science
Languages : en
Pages : 416

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A definitive reference, completely updated Published in 1989, the First Edition of this book, originally entitled Quadrupole Storage Mass Spectrometry, quickly became the definitive reference in analytical laboratories worldwide. Revised to reflect scientific and technological advances and new applications in the field, the Second Edition includes new chapters covering: * New ion trap instruments of high sensitivity * Peptide analysis by liquid chromatography/ion trap tandem mass spectrometry * Analytical aspects of ion trap mass spectrometry combined with gas chromatography * Simulation of ion trajectories in the ion trap One additional chapter discusses the Rosetta mission, a "comet chaser" that was sent on a ten-year journey in 2004 to study the comet Churyumov-Gerasimenko using, among other instruments, a GC/MS system incorporating a specially designed ion trap mass spectrometer. This comprehensive reference also includes discussions of the history of the quadrupole ion trap, the theory of quadrupole mass spectrometry, the dynamics of ion-trapping chemistry in the quadrupole ion trap, the cylindrical ion trap, miniature traps, and linear ion traps. Complete with conclusions and references, this primer effectively encapsulates the body of knowledge on quadrupole ion trap mass spectrometry. With its concise descriptions of the theory of ion motion and the principles of operation, Quadrupole Ion Trap Mass Spectrometry, Second Edition is ideal for new users of quadrupole devices, as well as for scientists, researchers, and graduate and post-doctoral students working in analytical laboratories.

Author: Claus Jacob
Publisher: Springer
ISBN: 9401789533
Category : Medical
Languages : en
Pages : 476

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Book Description
Nature endows us with a treasure chest of Green Gold full of amazing ‘redox-active’ substances which interfere with numerous biological processes in our own body, in animals, bacteria, fungi and plants. Whilst such natural products are all around and also in us, we still do not fully understand how these compounds actually work. This book attempts to resolve some of the mysteries and riddles associated with such products. Written by more than thirty international experts from academia and industry, it places a focus on modern developments in this field and considers such natural products from various angles, from their isolation and characterization all along to product development and commercialization. Throughout, the reader will be confronted with modern approaches which enable the efficient identification and isolation of new natural products, help to elucidate their mode(s) of action and permit practical uses in Medicine, Cosmetics, Agriculture, Industry and as functional foods.

Author: Mousumi Debnath
Publisher: Springer Science & Business Media
ISBN: 9048132614
Category : Medical
Languages : en
Pages : 527

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Book Description
A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.

Author: Challa S.S.R. Kumar
Publisher: Springer Nature
ISBN: 3662595966
Category : Technology & Engineering
Languages : en
Pages : 467

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Book Description
Ninth volume of a 40 volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about Nanotechnology Characterization Tools for Tissue Engineering and Medical Therapy. Modern applications and state-of-the-art techniques are covered and make this volume an essential reading for research scientists in academia and industry.

Author: Pier Francesco Bortignon
Publisher: Springer
ISBN: 3030016293
Category : Science
Languages : en
Pages : 313

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Book Description
This book is a collection of multidisciplinary papers presented at the Department of Physics of Milan University's congress on 28 and 29 June 2017, which was also intended as a kick-off meeting for the design of a novel science campus at the Expo site in Milan. The congress presented a snapshot of the department's research to the academic community, the media, policymakers and authorities as well as the public at large, and also provided an opportunity to strengthen interdisciplinary collaborations between the members of the department and other communities. This book is a valuable resource for scientists looking for synergetic projects, policymakers wanting to grasp scientists' points of view and for prospective graduate students seeking expanding areas of research.

Author: Timothy D. Veenstra
Publisher: John Wiley & Sons
ISBN: 0470007737
Category : Science
Languages : en
Pages : 361

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Book Description
Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.

Author: Yu Wai Chen
Publisher: Humana
ISBN: 9781627036900
Category : Medical
Languages : en
Pages : 0

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Book Description
The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.

Author: Ahmed Belasri
Publisher: Springer Nature
ISBN: 9811554447
Category : Technology & Engineering
Languages : en
Pages : 659

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Book Description
This book highlights peer reviewed articles from the 1st International Conference on Renewable Energy and Energy Conversion, ICREEC 2019, held at Oran in Algeria. It presents recent advances, brings together researchers and professionals in the area and presents a platform to exchange ideas and establish opportunities for a sustainable future. Topics covered in this proceedings, but not limited to, are photovoltaic systems, bioenergy, laser and plasma technology, fluid and flow for energy, software for energy and impact of energy on the environment.