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Author: Wade William Grabow Publisher: ISBN: 9781267767448 Category : Languages : en Pages : 275
Book Description
Over the last several decades ribonucleic acid (RNA) has been found to carry out a variety of previously unrecognized functions within cellular organisms. These discoveries, in conjunction with the first successful structural determination of natural RNA molecules by X-ray and NMR, have generated an interest in RNA outside the traditional bounds of molecular and cellular biology. The ability to control three-dimensional (3-D) space on the nano-scale as well as the inherent and demonstrated abilities of nucleic acids to self-assemble into predictable and controllable materials has caused RNA to gain acceptance and influence in the interdisciplinary fields of synthetic biology, nanotechnology, and nano-medicine. The following work details the guiding principles associated with the design and characterization of both natural and synthetic RNA while highlighting several newly designed RNA-based scaffolds with potential uses in the diverse fields above as well as the emerging field of RNA nanotechnology, which at its core seeks to design, to construct, and to test RNA nanoparticles that can perform specific cellular or biological functions.
Author: Wade William Grabow Publisher: ISBN: 9781267767448 Category : Languages : en Pages : 275
Book Description
Over the last several decades ribonucleic acid (RNA) has been found to carry out a variety of previously unrecognized functions within cellular organisms. These discoveries, in conjunction with the first successful structural determination of natural RNA molecules by X-ray and NMR, have generated an interest in RNA outside the traditional bounds of molecular and cellular biology. The ability to control three-dimensional (3-D) space on the nano-scale as well as the inherent and demonstrated abilities of nucleic acids to self-assemble into predictable and controllable materials has caused RNA to gain acceptance and influence in the interdisciplinary fields of synthetic biology, nanotechnology, and nano-medicine. The following work details the guiding principles associated with the design and characterization of both natural and synthetic RNA while highlighting several newly designed RNA-based scaffolds with potential uses in the diverse fields above as well as the emerging field of RNA nanotechnology, which at its core seeks to design, to construct, and to test RNA nanoparticles that can perform specific cellular or biological functions.
Author: Kirill A. Afonin Publisher: Springer Nature ISBN: 1071634178 Category : Science Languages : en Pages : 331
Book Description
This volume details protocols for computer-assisted design and experimental characterization of RNA nanostructures. Chapters guide readers through RNA nanotechnology, design and characterization of RNA nanostructures, assessment of immunology of nanomaterials, biosensing and drug delivery, various biomedical applications, and delivery approaches for therapeutic RNA nanoparticles. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, RNA Nanostructures: Design, Characterization, and Applications aims to address essential topics and concerns in the growing field of RNA nanotechnology.
Author: Peixuan Guo Publisher: Humana ISBN: 9781493954506 Category : Science Languages : en Pages : 0
Book Description
This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls. Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.
Author: Bin Wang Publisher: CRC Press ISBN: 9814411647 Category : Medical Languages : en Pages : 468
Book Description
In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.
Author: Bin Wang Publisher: ISBN: Category : Languages : en Pages :
Book Description
In the past few decades there has been incredible growth in "bionano"--Related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.
Author: Peixuan Guo Publisher: CRC Press ISBN: 1000552675 Category : Medical Languages : en Pages : 644
Book Description
Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, immunorecognition of RNA nanomaterials, RNA chemistry for nanoparticle synthesis, and conjugation and labeling. Presents the latest research and discoveries in RNA nanotechnology Features contributions from world-class experts in the field Covers RNA nanoparticles in therapeutics Describes self-assembled RNA nanoparticles
Author: Luc Ponchon Publisher: ISBN: 9781071614990 Category : Electronic books Languages : en Pages : 288
Book Description
This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field. .
Author: Paul J. Zakrevsky Publisher: ISBN: 9781339218533 Category : Languages : en Pages : 256
Book Description
RNA nanotechnology is a growing field of research that aims to harness the functional potential of RNA for specific applications. The function of an RNA can be directly related to the native structure of the molecule, defined by combinations of recurrent structural building blocks termed motifs. Long-range assembly motifs are of critical importance during RNA folding and assembly. These long-range modules are often required for a molecule to find and stabilize its native fold, and can provide a means for intermolecular recognition and assembly. In the research presented here, we work to characterize specific long-range interactions derived from various natural RNAs, with a goal to understand their roles in RNA assembly and function. Characterization of assembly modules was performed primarily through biophysical techniques utilizing rationally designed RNA scaffolds. By improving our understanding of natural RNA assembly modules, we hope to garner insights concerning molecular design and regulation which could be of use for future endeavors in RNA nanotechnology.
Author: Kirill A. Afonin Publisher: CRC Press ISBN: 1000263177 Category : Medical Languages : en Pages : 1229
Book Description
This collection of research articles and reviews covers the latest work in the design, delivery, dynamic abilities, and immune stimulation of RNA nanoparticles which have driven the utilization of their immunomodulatory properties. The unknown immune properties of nucleic acid nanoparticles have been a major hurdle in their adaptation until the works herein began assessing their structure-activity relationships. This collection chronologically follows the path of investigating the recognition of design components to implementing them into nucleic acid nanostructures. RNA nanotechnology is an emerging platform for therapeutics with increasing clinical relevance as this approach becomes more widely used and approved for the treatment of various diseases. The latest research aims to take advantage of RNA’s modular nature for the design of nanostructures which can interact with their environments to communicate programmed messages with intracellular pathways. In doing so, nanoparticles can be used to elicit or elude responses by the immune system as desired in conjunction with their therapeutic applications.
Author: Yubing Xie Publisher: CRC Press ISBN: 1439838690 Category : Medical Languages : en Pages : 694
Book Description
A thorough overview of nanobiotechnology and its place in advances in applied science and engineering, The Nanobiotechnology Handbook combines contributions from physics, bioorganic and bioinorganic chemistry, molecular and cellular biology, materials science, and medicine as well as from mechanical, electrical, chemical, and biomedical engineering to address the full scope of current and future developments. World-class experts discuss the role of nanobiotechnology in bioanalysis, biomolecular and biomedical nanotechnology, biosensors, biocatalysis and biofuel, and education and workforce development. It includes a companion CD that contains all figures in the book. The book begins with discussions of biomimetic nanotechnology, including a comprehensive overview of DNA nanostructure and DNA-inspired nanotechnology, aptamer-functionalized nanomaterials as artificial antibodies, artificial enzymes, molecular motors, and RNA structures and RNA-inspired nanotechnology. It shows how nanotechnology can be inspired by nature as well as adverse biological events in diagnostic and therapeutic development. From there, the chapters cover major important and widely used nanofabrication techniques, applications of nanotechnology for bioprocessing followed by coverage of the applications of atomic force microscopy (AFM), optical tweezers and nanofluidics as well as other nanotechnology-enabled biomolecular and cellular manipulation and detection. Focusing on major research trends, the book highlights the importance of nanobiotechnology to a range of medical applications such as stem cell technology and tissue engineering, drug development and delivery, imaging, diagnostics, and therapeutics. And with coverage of topics such as nanotoxicity, responsible nanotechnology, and educational and workforce development, it provides a unique overview and perspective of nanobiotechnology impacts from a researcher’s, entrepreneur’s, economist’s and educator’s point of view. It provides a resource for current applications and future development of nanobiotechnology.
Author: Wade William Grabow
Author: Wade William Grabow
Author: Kirill A. Afonin
Author: Peixuan Guo
Author: Bin Wang
Author: Bin Wang
Author: Peixuan Guo
Author: Luc Ponchon
Author: Paul J. Zakrevsky
Author: Kirill A. Afonin
Author: Yubing Xie