Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
Contributions to Embryology, Vol. 8, No. 24-26
Author: Contributions to Embryology, Vol. 8
Author: Contributions to Embryology Vol. 19
Author: Contributions to Embryology, 1915, Vol. 3: Nos. 7, 8, 9 (Classic Reprint)
Author: Carnegie Institution Of WashingtonPublisher: Forgotten Books
ISBN: 9781391781631
Category : Science
Languages : en
Pages : 118
Book Description
Excerpt from Contributions to Embryology, 1915, Vol. 3: Nos. 7, 8, 9 The history Of the different methods of injecting embryos as they have developed in this laboratory may be Of interest. The work was begun by Dr. Mall and has been con tinned by his pupils. At first the work was a direct development from the methods Of making injections Of the vascular system in adult animals, and this has been summarized by Dr. Flint (1900) in his article on the adrenal. After injecting the blood-vessels of the adrenal in the adult, Flint applied a similar technique to the vessels in fetal stages, namely, he tied a canula into the aorta and injected Prussian blue by means Of a gravity flask. For younger stages, he tied a canula into the apex Of the heart, and finally in still younger stages into the umbilical artery or vein. By this last procedure he succeeded in injecting embryos as small as to 3 cm. In length. The next step in the injection Of the embryos came in my study Of the lymphatic vessels Of the skin, which were filled with Prussian blue by means Of a fine hypodermic needle. In asmuch as lymphatic vessels can seldom be seen, good results are only Obtained by knowing the-level Of the lymphatic plexus in order to puncture the vessels. Extensive injections Of the lymphatics in stages before the valves develop can best be made by considering the lines Of growth, that is to say, by finding the position of larger trunks, which are the primary vessels Of an area and which drain an extensive zone. In these studies it became necessary to get the relation Of the lymphatics to the blood-vessels, and I found that by puncturing the liver directly with a syringe and filling it with fluid, an injection Of the main arteries could be made by means Of the heart-beat. Thus was emphasized the necessity Of having fresh embryos with the heart still beating. During the same work (1902) I repeated Budge's injection of the extra - embryonal coelom and the tissue space connected with it. To make these injections I used a glass canula connected by means of a rubber tube to a pressure flask Of a very low column Of mercury. This is easily arranged by putting a little mercury into a U-tube, one end Of which is attached through a rubber tube to the canula and the other to a rubber bulb which can be compressed by a screw-clamp. These injections were made with India ink, which flows more easily than Prussian blue, since it has a finer granule. This manipulation was watched under a compound microscope, a procedure which was first emphasized by W. G. Maccallum. Since then all injections Of the young embryos have been made with the aid Of the binocular microscope. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
Contributions to Embryology Vol. 18, Nos. 90-97
Author: Contributions to Embryology Vol. 7, Nos. 20-23
Author: Contributions to Embryology, Vol. 1
Author: Contributions to Embryology
Author: Carnegie Institution of WashingtonPublisher:
ISBN:
Category : Birds
Languages : en
Pages : 218
Book Description
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