Author: Arun Sharma
Publisher: Butterworth-Heinemann
ISBN: 1483100847
Category : Science
Languages : en
Pages : 724

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Book Description
Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.

Author: Archarna Sharma
Publisher: CRC Press
ISBN: 1000159477
Category : Science
Languages : en
Pages : 400

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Book Description
This laboratory manual covers the study of chromosomes in plants, animal and human systems, dealing with the protocols and principles involved. It caters to the requirements of scientists working laboratories, presenting details of the operational mechanism for use at the chromosome level.

Author: Mark C. Leake
Publisher: Humana
ISBN: 9781493936298
Category : Medical
Languages : en
Pages : 0

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Book Description
This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author: Kiichi Fukui
Publisher: CRC Press
ISBN: 9780849389191
Category : Science
Languages : en
Pages : 298

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Book Description
Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies. Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309038405
Category : Science
Languages : en
Pages : 128

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Book Description
There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.

Author: Tariq Ahmad Bhat
Publisher: Springer
ISBN: 8132236734
Category : Medical
Languages : en
Pages : 377

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Book Description
This book is a compilation of various chapters contributed by a group of leading researchers from different countries and covering up to date information based on published reports and personal experience of authors in the field of cytogenetics. Beginning with the introduction of chromosome, the subsequent chapters on organization of genetic material, karyotype evolution, structural and numerical variations in chromosomes, B-chromosomes and chromosomal aberrations provide an in-depth knowledge and easy understanding of the subject matter. A special feature of the book is the inclusion of a series of chapters on various types of chromosomal aberrations and their impact on breeding behaviour and crop improvement. The possible mechanism, their consequences and role in genetic analysis has been emphasized in these chapters. A few chapters have also been dedicated on various techniques routinely used in the laboratory by students and researchers. Each chapter ends with an extensive bibliography so that the students and researchers may find it relevant to consult more literature on the subject than a book of this size can offer. The book is intended to fulfill the needs of undergraduate and post graduate students of botany, zoology and agriculture besides, teachers and researchers engaged in the field of genetics, cytogenetics, and molecular genetics. In general the readers will find each chapter of the book informative and easy to understand.

Author: Marilyn S. Arsham
Publisher: John Wiley & Sons
ISBN: 1119061229
Category : Science
Languages : en
Pages : 1216

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Book Description
Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.

Author: Hanping Wang
Publisher: John Wiley & Sons
ISBN: 1119127270
Category : Technology & Engineering
Languages : en
Pages : 1969

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Book Description
Awarded Bookauthority's "Best Aquaculture Books of all Time" A comprehensive resource that covers all the aspects of sex control in aquaculture written by internationally-acclaimed scientists Comprehensive in scope, Sex Control in Aquaculture first explains the concepts and rationale for sex control in aquaculture, which serves different purposes. The most important are: to produce monosex stocks to rear only the fastest-growing sex in some species, to prevent precocious or uncontrolled reproduction in other species and to aid in broodstock management. The application of sex ratio manipulation for population control and invasive species management is also included. Next, this book provides detailed and updated information on the underlying genetic, epigenetic, endocrine and environmental mechanisms responsible for the establishment of the sexes, and explains chromosome set manipulation techniques, hybridization and the latest gene knockout approaches. Furthermore, the book offers detailed protocols and key summarizing information on how sex control is practiced worldwide in 35 major aquaculture species or groups, including fish and crustaceans, and puts the focus on its application in the aquaculture industry. With contributions from an international panel of leading scientists, Sex Control in Aquaculture will appeal to a large audience: aquaculture/fisheries professionals and students, scientists or biologists working with basic aspects of fish/shrimp biology, growth and reproductive endocrinology, genetics, molecular biology, evolutionary biology, and R&D managers and administrators. This text explores sex control technologies and monosex production of commercially-farmed fish and crustacean species that are highly in demand for aquaculture, to improve feed utilization efficiency, reduce energy consumption for reproduction and eliminate a series of problems caused by mixed sex rearing. Thus, this book: Contains contributions from an international panel of leading scientists and professionals in the field Provides comprehensive coverage of both established and new technologies to control sex ratios that are becoming more necessary to increase productivity in aquaculture Includes detailed coverage of the most effective sex control techniques used in the world's most important commercially-farmed species Sex Control in Aquaculture is the comprehensive resource for understanding the biological rationale, scientific principles and real-world practices in this exciting and expanding field.

Author: R.J. MKinlay Gardner
Publisher: OUP USA
ISBN: 0195375335
Category : Medical
Languages : en
Pages : 650

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Book Description
Advances in cytogenetics continue to crop up in wonderful ways, and we know exponentially more about chromosomes now than mere decades ago. Likewise, the necessary skills in offering genetic counseling continue to evolve. This new edition of Chromosome Abnormalities in Genetic Counseling offers a practical, up-to-date guide for the genetic counselor to marshal cytogenetic data and analysis clearly and effectively to families.

Author: Beatrice Bodega
Publisher: Humana
ISBN: 9781071606636
Category : Science
Languages : en
Pages : 322

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Book Description
This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.