Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence PDF Download

Author:
Publisher: Academic Press
ISBN: 0128211547
Category : Science
Languages : en
Pages : 344

View

Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways

Author:
Publisher: Academic Press
ISBN: 0128211520
Category : Science
Languages : en
Pages : 432

View

Book Description
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways

Author:
Publisher: Academic Press
ISBN: 0128211555
Category : Science
Languages : en
Pages : 526

View

Book Description
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing ß-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways

Author:
Publisher: Academic Press
ISBN: 0128201487
Category : Science
Languages : en
Pages : 360

View

Book Description
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways

Author: National Research Council
Publisher: National Academies Press
ISBN: 030916463X
Category : Science
Languages : en
Pages : 222

View

Book Description
Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.

Author: Baldassare Di Bartolo
Publisher: Springer
ISBN: 9789400700284
Category : Science
Languages : en
Pages : 434

View

Book Description
This volume describes an impressive array of the current photonic-related technologies being used in the investigation of biological systems. The topics include various types of microscopy (fluorescence correlation microscopy, two-photon microscopy), sensitive detection of biological molecules, nano-surgery techniques, fluorescence resonance energy transfer, nano-plasmonics, terahertz spectroscopy, and photosynthetic energy conversion. The emphasis is on the physical principles behind each technique, and on examining the advantages and limitations of each.The book begins with an overview by Paras Prasad, a leader in the field of biophotonics, of several important optical techniques currently used for studying biological systems. In the subsequent chapters these techniques are discussed in depth, providing the reader with a detailed understanding of the basic physical principles at work. An excellent treatment of terahertz spectroscopy demonstrates how photonics is being extended beyond the visible region. Recent results in the use of femtosecond lasers as a tool to porate cell walls demonstrate that the manipulation of light can be used as a tool for the study and the treatment of biological systems. The field of Bio-photonics is broad and still growing, so cannot be covered comprehensively in one volume. But here the reader will find an introduction to some of the major tools used for studying biological systems, and at the same time a detailed, first-principles treatment of the physics behind these tools.

Author: Zhen Cheng
Publisher: Springer Nature
ISBN: 3030467074
Category : Science
Languages : en
Pages : 230

View

Book Description
This book reviews the most recent developments of fluorescent imaging techniques for medicinal chemistry research and biomedical applications, including cell imaging, in vitro diagnosis and in vivo imaging. Fluorescent imaging techniques play an important role in basic research, drug discovery and clinical translation. They have great impact to many fields including chemical biology, cell biology, medical imaging, cancer diagnosis and treatment, pharmaceutical science, among others, and they have facilitated our understanding of diseases and helped to develop many novel powerful tools for imaging and treatment of diseases. This book will appeal to scientists from numerous fields such as chemistry, pharmaceutical science, biology, materials science, and medicine, and it will serve as a very useful and handy resource for readers with different levels of scientific knowledge, ranging from entry level to professional level.

Author: Nicholas Long
Publisher: John Wiley & Sons
ISBN: 1118854772
Category : Technology & Engineering
Languages : en
Pages : 408

View

Book Description
Molecular imaging is primarily about the chemistry of novelbiological probes, yet the vast majority of practitioners are notchemists or biochemists. This is the first book, written from achemist's point of view, to address the nature of the chemicalinteraction between probe and environment to help elucidatebiochemical detail instead of bulk anatomy. Covers all of the fundamentals of modern imaging methodologies,including their techniques and application within medicine andindustry Focuses primarily on the chemistry of probes and imagingagents, and chemical methodology for labelling andbioconjugation First book to investigate the chemistry of molecularimaging Aimed at students as well as researchers involved in the areaof molecular imaging

Author: Raquel Seruca
Publisher: CRC Press
ISBN: 1351647334
Category : Medical
Languages : en
Pages : 274

View

Book Description
This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.

Author: Andrew Cavell
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

View

Book Description
Fluorescence microscopy has proven itself to be a powerful yet flexible platform for making measurements on many kinds of chemical systems. The application of fluorescence microscopy to biological systems has enabled decades of new discoveries and diagnostic protocols, while applications in the field of chemistry have ramped up more slowly but yielded discoveries unreachable by other means. The ability to make observations at low background and high sensitivity in real time enables the monitoring of molecular-scale processes occurring in quite complicated and noisy environments, without the disruption to reaction dynamics that other techniques might introduce. The inherent simplicity and scalability of many of these technologies makes them poised for use in a variety of fields for new in situ measurements and high-throughput screening. Still, the idiosyncrasies of each system require some work to fit the instrument to the desired measurement. In this thesis, I demonstrate the application of fluorescence microscopy to monitor three different chemical systems using three different optical setups, each tuned to the specific needs of the work at hand. While the principles of operation remain the same, the information produced is quite different, relying on various photophysical mechanisms and varied resulting design considerations. These are reviewed in the introductory chapter along with an overview of fluorescence itself, and then each is described in detail in a chapter corresponding to each project: First, two parallel measurements are shown to act as complementary readouts for monitoring polymerization catalysis confined in droplets of and the combination of two methods yields an enhanced temporal dynamic range. Second, the output of an array of electrochemical reactions is monitored with a pH sensitive fluorescent dye in order to realize an implementation of a hybrid classical-molecular computing platform. Finally, the release of lithium ions is tracked using fluorescence during the charging of a lithium-ion battery cathode material, with relationships between fluorescence signal, lithium ion release, and particle morphology explored.