Application of PCR Technologies for Virus Detection in Groundwater PDF Download

Author: Morteza Abbaszadegan
Publisher: American Water Works Association
ISBN: 0898679346
Category : Enteroviruses
Languages : en
Pages : 86

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Book Description
Presents a study of the application of the polymerase chain reaction (PCR) for the detection of enteric viruses in groundwater, in which the occurrence of enteric viruses in 150 groundwater samples was determined, as was the possible association of virus presence with several potential biological and physical indicators. No index. Annotation copyrighted by Book News, Inc., Portland, OR

Author: United States. Congress. House. Committee on Science. Subcommittee on Energy and Environment
Publisher:
ISBN:
Category : Environmental protection
Languages : en
Pages : 1434

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Author: United States. Congress. House. Committee on Science. Subcommittee on Energy and Environment
Publisher:
ISBN:
Category : Law
Languages : en
Pages : 1612

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Author: National Research Council
Publisher: National Academies Press
ISBN: 0309172969
Category : Nature
Languages : en
Pages : 276

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Book Description
With an increasing population, use of new and diverse chemicals that can enter the water supply, and emergence of new microbial pathogens, the U.S. federal government is faced with a regulatory dilemma: Where should it focus its attention and limited resources to ensure safe drinking water supplies for the future? Identifying Future Drinking Water Contaminants is based on a 1998 workshop on emerging drinking water contaminants. It includes a dozen papers that were presented on new and emerging microbiological and chemical drinking water contaminants, associated analytical and water treatment methods for their detection and removal, and existing and proposed environmental databases to assist in their proactive identification and regulation. The papers are preceded by a conceptual approach and related recommendations to EPA for the periodic creation of future Drinking Water Contaminant Candidate Lists (CCLsâ€"produced every five yearsâ€"include currently unregulated chemical and microbiological substances that are known or anticipated to occur in public water systems and that may pose health risks).

Author: Moselio Schaechter
Publisher: Elsevier
ISBN: 008047246X
Category : Science
Languages : en
Pages : 1176

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Book Description
The Desk Encyclopedia of Microbiology aims to provide an affordable and ready access to a large variety of microbiological topics within one set of covers. This handy desk-top reference brings together an outstanding collection of work by the top scientists in the field. Covering topics ranging from the basic science of microbiology to the current "hot" topics in the field.* Provides a broad, easily accessible perspective on a wide range of microbiological topics* A synthesis of the broadest topics from the comprehensive and multi-volumed Encyclopedia of Microbiology, Second Edition * Helpful resource in preparing for lectures, writing reports, or drafting grant applications

Author: Sarah V. Thomas
Publisher: Nova Publishers
ISBN: 9781604562088
Category : Nature
Languages : en
Pages : 298

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Book Description
Pollution is undesirable state of the natural environment being contaminated with harmful substances as a consequence of human activities so that the environment becomes harmful or unfit for living things; especially applicable to the contamination of soil, water, or the atmosphere by the discharge of harmful substances. In addition to the harm to living beings, both present or future and known or unknown, pollution cleanup and surveillance are enormous financial drains of the economies of the world. This book focuses on issues and developments critical for the field.

Author: Kelly A. Reynolds
Publisher: American Water Works Association
ISBN: 1583211012
Category : Drinking water
Languages : en
Pages : 90

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Book Description
This volume presents the results of a study which used a combination integrated cell culture/PCR (ICC/PCR) technique for detecting human enteric viruses in raw and finished drinking water. The technique allows for definitive detection of infectious enteroviruses in days compared to weeks with cell culture alone. In addition, ICC/PCR eliminates the traditional flaw of PCR analysis alone, which cannot distinguish between infectious virus versus noninfectious virus. The text describes the materials and methods used, reports the results in testing for poliovirus and Hepatitis A, and discusses recommendations for water utilities. The volume is not indexed. c. Book News Inc.

Author: Gary A. Toranzos
Publisher: CRC Press
ISBN: 1040288987
Category : Science
Languages : en
Pages : 240

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Book Description
From the Preface Antibody techniques have allowed us to study microorganisms in situ. However, until recently all methodology lacked the sensitivity necessary for environmental work where microorganisms are in most cases present at very low concentrations or where microbial ecosystems contain a myriad of different organisms. Gene probes have been used successfully for a variety of samples, but this method was still not sensitive enough. The next logical step was the application of the recently developed DNA amplification technique known as the polymerase chain reaction, or PCR. Since then, many laboratories around the world have adopted PCR for environmental work. Samples obtained from soils, water and air are enormously complex because they are unknown mixtures of DNA and other compounds. Thus, procedures for target DNA amplification from the environment require special attention. The PCR has allowed us to go beyond the need for culturing prior to analysis of microbial communities. It has been shown that even microorganisms that can be routinely grown in the laboratory undergo some physiological changes when exposed to the environment. One of these changes (first observed by R. Colwell and colleagues) is known as the viable-but-non-culturable state, and seems to be a common occurrence. Thus, the use of culture techniques paint only part of the picture in terms of microbial behavior under environmental conditions. The ability to amplify nucleic acids by the PCR has brought about a myriad of very ingenious modifications to the technique that can then be used to study complex ecosystems. The manner in which the PCR can be modified is only limited by the need and/or the imagination of the researcher. The first manual dedicated specifically to the analysis (by PCR) of environmental samples, Environmental Applications of Nucleic Acid Amplification Techniques presents state of the art methodology for the detection of microorganisms in soil, water, air samples, as well as the amplification of nucleic acids from fossil samples. The manual gives step-by-step procedures for the analysis of these samples. Although several publications have addressed the use of Polymerase Chain Reaction technique, very few of them have been directed toward the application of this technique to environmental samples. This book fills this gap in the literature.

Author:
Publisher:
ISBN:
Category : Water
Languages : en
Pages : 962

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Author: National Research Council
Publisher: National Academies Press
ISBN: 0309091225
Category : Nature
Languages : en
Pages : 329

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Book Description
Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens. Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples. This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application. The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â