Microscopy, Immunohistochemistry, and Antigen Retrieval Methods PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Microscopy, Immunohistochemistry, and Antigen Retrieval Methods PDF full book. Access full book title Microscopy, Immunohistochemistry, and Antigen Retrieval Methods by M.A. Hayat. Download full books in PDF and EPUB format.
Author: M.A. Hayat Publisher: Springer Science & Business Media ISBN: 0306475995 Category : Medical Languages : en Pages : 368
Book Description
Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
Author: M.A. Hayat Publisher: Springer Science & Business Media ISBN: 0306475995 Category : Medical Languages : en Pages : 368
Book Description
Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
Author: Shuji Yamashita Publisher: ISBN: Category : Medical Languages : en Pages : 0
Book Description
Heat-induced antigen retrieval (HIAR) method reported by Shi et al. in 1991 has greatly contributed not only to immunohistochemistry but also to studying gene expressions using archived formalin-fixed and paraffin-embedded (FFPE) specimens. Heating cleaves crosslinks (methylene bridges) in formaldehyde-fixed proteins and extends polypeptides to expose epitopes hidden in the inner portion of antigens or covered by adjacent macromolecules. In this chapter, the following topics are described to reconsider the concept of immunohistochemistry flexibly and to apply HIAR for further immunological studies using a variety of specimens: (1) antigen-antibody interactions in tissues; (2) mechanisms of chemical fixation with formaldehyde, glutaraldehyde, and osmium tetroxide; (3) unmasking of epitopes using HIAR for specimens fixed with chemical fixatives, including highly masked epitopes; (4) a standardized fixative for immunoelectron microscopy-based HIAR; (5) HIAR for conventionally processed electron microscopy specimens embedded in epoxy resins; and (6) effects of antibody diluents on immunohistochemistry.
Author: Igor B. Buchwalow Publisher: Springer Science & Business Media ISBN: 3642046096 Category : Medical Languages : en Pages : 153
Book Description
This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Author: Julia M. Polak Publisher: Taylor & Francis ISBN: Category : Medical Languages : en Pages : 164
Book Description
Immunocytochemical techniques are essential in diagnosis and biomedical research. This rewritten and updated introduction for beginners explains the reasons for the various steps, and discusses problems for specificity and sensitivity and their solutions. It includes practical instructions for several immunostaining methods using flourescent, enzyme and gold labels.
Author: Charles Streckfus Publisher: BoD – Books on Demand ISBN: 1838808205 Category : Medical Languages : en Pages : 142
Book Description
Immunohistochemistry - The Ageless Biotechnology is a book that is ideal for undergraduate and graduate biomedical researchers, and medical and dental health professionals. It is a detailed text, which emphasizes the laboratory and clinical implications of immunohistochemistry. The text covers the advances of immunohistochemistry from its humble origins in the 1930s up to the new decade of 2020. The book also offers a review of the immunohistochemistry detection systems with emphasis on their principles, history, and their advantages. It also stipulates the limitations and delineates the factors that need to be considered for choosing an appropriate detection system for IHC applications. The book describes current laboratory techniques and new applications for the technology. As the reader will observe, the book provides new and useful information concerning the rapidly advancing field of immunohistochemistry.
Author: Carlo Pellicciari Publisher: Springer Nature ISBN: 1071626752 Category : Science Languages : en Pages : 351
Book Description
This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.
Author: Simon Renshaw Publisher: John Wiley & Sons ISBN: 1118717775 Category : Science Languages : en Pages : 258
Book Description
Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
Author: M.A. Hayat
Author: M.A. Hayat
Author: Shuji Yamashita
Author: M. A. Hayat
Author: Igor B. Buchwalow
Author: Shan-Rong Shi
Author: Julia M. Polak
Author: Christopher Guerin
Author: Charles Streckfus
Author: Carlo Pellicciari
Author: Simon Renshaw