A Genome Wide Association Study for Fusarium Head Blight Resistance in Southern Soft Red Winter Wheat PDF Download
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Author: Amanda Leigh Holder Publisher: ISBN: Category : Fusarium diseases of plants Languages : en Pages : 162
Book Description
Fusarium head blight (FHB) is a disease of small grains caused by the fungal pathogen Fusarium graminearum. FHB poses potential economic losses and health risks due to the accumulation of the mycotoxin deoxynivalenol (DON) on infected seed heads. The objectives of this study are: 1) evaluate soft red winter wheat (SRWW) lines for resistance to FHB in terms of resistance to initial inoculum (incidence); resistance to spread within the head (severity); resistance to DON accumulation; and resistance to Fusarium damaged kernels (FDK), 2) determine the frequency and effect of known FHB resistance genes and quantitative trait loci (QTL), and 3) identify novel resistance loci using a genome wide association (GWA) approach. From 2014-2017, 360 SRWW breeding lines were evaluated in inoculated misted FHB nurseries in Fayetteville and Newport, AR and Winnsboro, LA (2017 only) in a randomized complete block design. At all locations, lines were sown in two row plots, inoculated with F. graminearum infected corn (Zea mays L.) and overhead misted throughout the months of April and May to provide optimal conditions for FHB infection. In addition to visual ratings and DON analysis, lines were screened with KASP® markers linked to known FHB resistance genes, including Fhb1. The known resistance QTL, Qfhb.nc-2B.1 (Bess), on chromosome 3B was significantly associated with a reduction in incidence, severity, and DON accumulation. Genome wide SNP markers generated through genotype by sequencing (GBS) were used to perform GWA in order to identify marker-trait associations for FHB resistance. The GWA analysis identified 58 highly significant SNPs associated with the four disease traits. The most highly significant SNP was found on chromosome 4A and the minor allele was found to significantly reduce incidence by 1.17%. Results from this study will facilitate the development of SRWW cultivars with improved resistance to FHB.
Author: Amanda Leigh Holder Publisher: ISBN: Category : Fusarium diseases of plants Languages : en Pages : 162
Book Description
Fusarium head blight (FHB) is a disease of small grains caused by the fungal pathogen Fusarium graminearum. FHB poses potential economic losses and health risks due to the accumulation of the mycotoxin deoxynivalenol (DON) on infected seed heads. The objectives of this study are: 1) evaluate soft red winter wheat (SRWW) lines for resistance to FHB in terms of resistance to initial inoculum (incidence); resistance to spread within the head (severity); resistance to DON accumulation; and resistance to Fusarium damaged kernels (FDK), 2) determine the frequency and effect of known FHB resistance genes and quantitative trait loci (QTL), and 3) identify novel resistance loci using a genome wide association (GWA) approach. From 2014-2017, 360 SRWW breeding lines were evaluated in inoculated misted FHB nurseries in Fayetteville and Newport, AR and Winnsboro, LA (2017 only) in a randomized complete block design. At all locations, lines were sown in two row plots, inoculated with F. graminearum infected corn (Zea mays L.) and overhead misted throughout the months of April and May to provide optimal conditions for FHB infection. In addition to visual ratings and DON analysis, lines were screened with KASP® markers linked to known FHB resistance genes, including Fhb1. The known resistance QTL, Qfhb.nc-2B.1 (Bess), on chromosome 3B was significantly associated with a reduction in incidence, severity, and DON accumulation. Genome wide SNP markers generated through genotype by sequencing (GBS) were used to perform GWA in order to identify marker-trait associations for FHB resistance. The GWA analysis identified 58 highly significant SNPs associated with the four disease traits. The most highly significant SNP was found on chromosome 4A and the minor allele was found to significantly reduce incidence by 1.17%. Results from this study will facilitate the development of SRWW cultivars with improved resistance to FHB.
Author: Sampurna Bartaula Publisher: ISBN: Category : Languages : en Pages :
Book Description
Fusarium head blight (FHB) is a devastating wheat (Triticum aestivum L.) disease worldwide. Presently, there is insufficient FHB resistance in the Canadian wheat germplasm. Genome-wide association study (GWAS) and genomic selection (GS) can be utilized to identify sources of resistance that could benefit wheat breeding. To define the genetic architecture of FHB resistance, association panels from a spring and a winter collection were evaluated using the Wheat Illumina Infinium 90K array. A total of 206 accessions from the spring panel and 73 from the winter panel were evaluated in field trials for 3-4 years at two locations, namely Morden (Manitoba) and Ottawa (Ontario). These accessions were phenotyped for FHB incidence (INC), severity (SEV), visual rating index (VRI), and deoxynivalenol (DON) content. Significant (p
Author: Harwinder Sidhu Publisher: ISBN: Category : Languages : en Pages :
Book Description
Fusarium Head Blight (FHB) is a devastating disease of Wheat (Triticum aestivum L.) caused primarily by Fusarium graminearum Schwabe in Canada. Wheat FHB results in yield and produce quality losses. The clearest symptoms of FHB are premature bleaching of spikelets in the field, and fusarium damaged kernels in the harvested seed. Insufficient and costly disease control strategies make breeding for FHB resistance in wheat an ideal choice. There is a lack of FHB resistance sources in the Canadian wheat germplasm and Genome Wide Association Studies (GWAS) can be utilized to identify such sources. Furthermore, as no Quantitative Trait Loci (QTL) provides absolute resistance to FHB, identification of novel sources of resistance is desired. Genomic Selection (GS) has great potential in crop improvement, specifically for quantitative traits. A diversity panel that represents the genetic diversity of the Canadian, i.e., high latitude North American winter wheat was assembled for this thesis and the genetic diversity, population structure, and linkage disequilibrium of the germplasm was studied. To identify QTL associated with FHB, a GWAS study was conducted using the phenotypic data collected at three locations over two years. For understanding the role of number of genotypic markers, population structure, and choice of model in trait prediction modelling, a GS study was conducted. The Canadian Winter Wheat Diversity Panel is a diverse collection of winter wheat capturing diversity both in time and geography from Canada. The panel comprises of seven subpopulations with different LD, allele frequencies, and genetic diversity parameters. Multiple QTL associated with FHB related traits were identified on 13 chromosomes which may harbor genes involved in plant defense and stress response mechanisms. For FHB resistance improvement, the GS study demonstrated that the modelling parameters can be determined based upon the genotypic information available. Deoxynivalenol contamination was one of the traits with the highest prediction accuracy. GS can account for minor effect QTL, which is beneficial when breeding for quantitative traits. FHB resistant varieties are needed for an effective and economical disease control strategy in wheat and GS can complement current breeding efforts to develop FHB resistant wheat varieties.
Author: Łukasz Stępień Publisher: MDPI ISBN: 303943408X Category : Science Languages : en Pages : 262
Book Description
It has been over 200 years since Fusarium pathogens were described for the first time, and they are still in the spotlight of researchers worldwide, mostly due to the mycotoxigenic abilities and the subsequent introduction of harmful metabolites into the food chain. The accelerating climatic changes are resulting in pathogen population and chemotype shifts all around the world, thus increasing the demand for continuous studies of factors that affect the virulence, disease severity and mycotoxin accumulation in plant tissues. This Special Issue summarizes recent advances in the field of Fusarium genetics, biology and toxicology.
Author: Yaoguang Li Publisher: ISBN: Category : Languages : en Pages :
Book Description
Wheat Fusarium head blight (FHB) is a devastating disease of wheat worldwide, which can significantly reduce grain yield and quality. Although the application of fungicides can reduce FHB damage, growing FHB resistant wheat is the most effective and eco-friendly approach to reduce the losses. To develop locally adapted FHB-resistant hard winter wheat germplasm, we transferred three major QTLs: Fhb1, Qfhs.ifa-5A, and Qfhb.rwg-5A.2 into two hard winter wheat cultivars, 'Everest' and 'Overland', using marker-assisted backcrossing and multiplex restriction amplicon sequencing (MRASeq). Ten 'Overland' background lines and nine 'Everest' background lines with better FHB resistance, recurrent parent similar agronomic traits were selected. They can be used as FHB resistant bridge parents for hard winter wheat breeding. To identify native FHB resistant sources, a population of 201 U.S. breeding lines and cultivars were genotyped using 90K wheat SNP arrays and phenotyped for the percentage of symptomatic spikelets (PSS), Fusarium damaged kernels (FDK) and deoxynivalenol (DON), a toxin produced by the pathogen. Genome-wide association studies (GWAS) identified significant trait associations with single nucleotide polymorphisms (SNPs) on chromosomes 1A, 1D, 2B, 3A, 3B, 4A, 5B and 5D. These marker-trait associations (MTAs) were significant for at least two of the three traits or a single trait in at least two experiments. To accelerate the evaluation of the FDK, we developed an algorithm that can separate FDK from healthy kernels with an accuracy of 90% based on color differences using image processing and unsupervised machine learning methods. Discovery and creation of the new FHB resistant germplasms and development of the fast FDK phenotyping algorithm will accelerate the improvement of U.S. hard winter wheat cultivars for FHB resistance.
Author: Amanda Leigh Holder
Author: Amanda Leigh Holder
Author: Sampurna Bartaula
Author: Harwinder Sidhu
Author: Jared Howell Benson
Author: Łukasz Stępień
Author: Yaoguang Li
Author: Virginia Laura Verges
Author: Nusret Zencirci
Author: Marla Dale Hall
Author: Daniela Sarti Dvorjak