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Author: Biji T. Kurien Publisher: Springer Nature ISBN: 3030706842 Category : Medical Languages : en Pages : 467
Book Description
This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..
Author: Biji T. Kurien Publisher: Springer Nature ISBN: 3030706842 Category : Medical Languages : en Pages : 467
Book Description
This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..
Author: Akash Gautam Publisher: Springer Nature ISBN: 3030942309 Category : Science Languages : en Pages : 203
Book Description
This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.
Author: Christine Goetz Publisher: Springer ISBN: 3319980718 Category : Medical Languages : en Pages : 219
Book Description
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.
Author: Biji T. Kurien Publisher: Humana ISBN: 9781493926930 Category : Science Languages : en Pages : 0
Book Description
This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.
Author: Lisa A. Seidman Publisher: CRC Press ISBN: 1000480763 Category : Medical Languages : en Pages : 1210
Book Description
Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.
Author: Dr Jagjit Singh Publisher: Taylor & Francis ISBN: 1135824622 Category : Architecture Languages : en Pages : 327
Book Description
Biodeterioration is the most common problem of mankind and its record can be traced back to biblical times or probably earlier. Building Mycology deals with the study of fungi in and around the building environment, having direct and indirect effect on the health of the building, its materials, structures and occupants.
Author: Alexander E. Kalyuzhny Publisher: Springer ISBN: 3319308939 Category : Science Languages : en Pages : 91
Book Description
Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.
Author: Ann Arvin Publisher: Cambridge University Press ISBN: 1139461648 Category : Medical Languages : en Pages : 1325
Book Description
This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Author: Richard D. Urman Publisher: Springer Science & Business Media ISBN: 1461405513 Category : Medical Languages : en Pages : 380
Book Description
Increasingly, physicians are leveraging their medical training and expertise to pursue careers in non-traditional arenas. Their goals are diverse: · Explore consulting as a way to improve patient care · Lay the foundation for a career in academic medicine · Provide leadership in healthcare · Strengthen ties between a clinic and the community · Broaden one’s experience as a medical student · As a journalist or writer, open a window onto medicine for non-experts Some physicians will pursue another degree, while others may not, in anticipation of moving into public service, business, education, law, or organized medicine. Their common ground is the desire to enhance their professional fulfillment. Drs. Urman and Ehrenfeld’s book features individual chapters on the wide array of non-traditional careers for physicians, each one written by an outstanding leader in medicine who him- or herself has successfully forged a unique career path. A final chapter brings together fascinating brief profiles – “case studies” – of physicians who have distinguished themselves professionally outside of traditional settings. Suitable for readers at any point in their medical career – practitioners, fellows, residents, and medical students – who want to explore possibilities beyond traditional medical practice, the book also sets out common-sense advice on topics such as work-life balance, mentorship, and the relationship between personality and job satisfaction.
Author: Max Schweidler Publisher: Getty Publications ISBN: 9780892368358 Category : Books Languages : en Pages : 320
Book Description
Ever since its original publication in Germany in 1938, Max Schweidler's Die Instandetzung von Kupferstichen, Zeichnungen, Buchern usw has been recognized as a seminal modern text on the conservation and restoration of works on paper. To address what he saw as a woeful dearth of relevant literature and in order to assist those who have 'set themselves the goal of preserving cultural treasures, ' the noted German restorer composed a thorough technical manual covering a wide range of specific techniques, including detailed instructions on how to execute structural repairs and alterations that, if skilfully done, can be virtually undetectable. By the mid-twentieth century, curators and conservators of graphic arts, discovering a nearly invisible repair in an old master print or drawing, might comment that the object had been 'Schweidlerized.' This volume, based on the authoritative revised German edition of 1949, makes Schweidler's work available in English for the first time, in a meticulously edited and annotated critical edition. The editor's introduction places the work in its historical context and probes the philosophical issues the book raises, while some two hundred annotati
Author: Biji T. Kurien
Author: Biji T. Kurien
Author: Akash Gautam
Author: Christine Goetz
Author: Biji T. Kurien
Author: Lisa A. Seidman
Author: Dr Jagjit Singh
Author: Alexander E. Kalyuzhny
Author: Ann Arvin
Author: Richard D. Urman
Author: Max Schweidler