Ultracentrifugal Analysis in Theory and Experiment PDF Download

Author: J. W. Williams
Publisher: Elsevier
ISBN: 1483221229
Category : Science
Languages : en
Pages : 301

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Book Description
Ultracentrifugal Analysis: In Theory and Experiment aims to tackle some outstanding problems in sedimentation analysis. The book presents topics such as the thermodynamics of diffusion and sedimentation; diffusion and sedimentation in multicomponent systems; and the frictional formalism in the flow equations of sedimentation. The text also includes topics such as solutions of the general differential equation for the ultracentrifuge; the interpolation diagram for calculating model Schlieren patterns for reversibly interacting systems; and sedimentation of reversibly aggregating substances. Articles on the effects of charge on the sedimentation, the diffusion and the sedimentation equilibrium of colloidal electrolytes; the basic equilibrium equations; and the sedimentation equilibrium in reacting systems are also considered. The book further tackles articles on the optical systems for sedimentation analysis; computational methods of ultracentrifugation; separation cells; and the magnetic bearing for an ultracentrifuge. Chemists, physicists, and biologists will find the book invaluable.

Author: National Academy of Sciences (U.S.)
Publisher:
ISBN:
Category : Centrifuges
Languages : en
Pages : 282

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Book Description

Author: Peter Schuck
Publisher: CRC Press
ISBN: 1498751164
Category : Medical
Languages : en
Pages : 318

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Book Description
Analytical ultracentrifugation (AUC) can supply rich information on the mass, shape, size distribution, solvation, and composition of macromolecules and nanoscopic particles. It also provides a detailed view of their reversible single- or multi-component interactions over a wide range of affinities. Yet this powerful technique has been hard to mast

Author: David Scott
Publisher: Royal Society of Chemistry
ISBN: 1847552617
Category : Science
Languages : en
Pages : 613

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Book Description
Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.

Author: H. Cölfen
Publisher: Springer
ISBN: 3540487034
Category : Technology & Engineering
Languages : en
Pages : 233

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Book Description
The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.

Author: Todd M. Schuster
Publisher: Springer Science & Business Media
ISBN: 1468468286
Category : Science
Languages : en
Pages : 359

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Book Description
There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.

Author: Curtis A. Williams
Publisher: Academic Press
ISBN: 1483222993
Category : Medical
Languages : en
Pages : 480

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Book Description
Methods in Immunology: Volume II, Physical and Chemical Methods is a collection of papers dealing with electrophoresis, analytical ultracentrifugation, dialysis, ultrafiltration, cellulose ion exchangers, and chromatographic separation of macromolecules on porous gels. Some papers explain the applications of radioisotopes, optical analysis, and chemical analysis of proteins, carbohydrates, lipids, and nucleic acid. One paper describes the theory of electro-migration. Factors such as electrical charge or frictional coefficients govern the rate of migration of charged particles in an electric field. The differences found in their velocities can be used to separate substances or analyze them. Mobility is a characteristic property of molecules and can also be influenced by the composition of the medium or solution. Dialysis separates solvents too large to diffuse through a barrier from smaller solutes; ultrafiltration (reverse osmosis) forces solvent and solutes up to a certain critical size through the barrier by a high pressure on one side. The book notes that the membrane never becomes plugged in dialysis because of some opposite movement of the solvent. Another paper points out that the significance of radioactive tracers in immunochemistry employed to identify and label macromolecules functioning as antigens and antibodies. The collection can prove valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in immunological research.

Author:
Publisher: Academic Press
ISBN: 0080859984
Category : Science
Languages : en
Pages : 617

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Book Description
Molecular Structure and Dynamics

Author: Dan H. Moore
Publisher: Academic Press
ISBN: 1483194884
Category : Science
Languages : en
Pages : 345

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Book Description
Physical Techniques in Biological Research, Second Edition, Volume II, Part B: Physical Chemical Techniques covers physical chemical techniques that have been most widely employed in the investigation of molecules of biological significance. The book discusses the measurement and properties of ionizing radiations; magnetic resonance and related techniques in magnetic susceptibility measurements; and chromatographic methods and applications of chromatography to steroid research. The text then describes the phenomenon of centrifugation, including the techniques for studying density gradient centrifugation; the formation of gradient column in swinging-bucket tubes; gradient materials; density gradient centrifugation in heavy salt; some factors influencing the distribution of sedimenting particles; and the recovery of samples. Biological investigators and students of biophysics or biochemistry will find the book invaluable.

Author: Hans-Georg Elias
Publisher: Springer Science & Business Media
ISBN: 1461573645
Category : Technology & Engineering
Languages : en
Pages : 1228

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Book Description
Like so many of its kind, this textbook originated from the requirements of teaching. While lecturing on macromolecular science as a required subject for chemists and materials scientists on the undergraduate, graduate, and postgraduate levels at Swiss Federal Institute of Technology at Zurich (1960-1971), I needed a one-volume textbook which treated the whole field of macromolecular science, from its chemistry and physics to its applications, in a not too elementary manner. This textbook thus intends to bridge the gap between the often oversimplified introductory books and the highly specialized texts and monographs that cover only parts of macromolecular science. This first English edition is based on the third German edition (1975), which is about 40% different from the first German edition (1971), a result of rapid progress in macromolecular science and the less rapid education of the writer. This text intends to survey the whole field of macromolecular science. Its organization results from the following considerations. The chemical structure of macromolecular compounds should be independent of the method of synthesis, at least in the ideal case. Part I is thus concerned with the chemical and physical structure of macro molecules. Properties depend on structure. Solution properties are thus discussed in Part II, solid state properties in Part III. There are other reasons for discussing properties before syntheses: For example, it is difficult to under stand equilibrium polymerization without knowledge of solution thermody of the glass temperature, etc.