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Cell-Free Protein Production

Cell-Free Protein Production PDF Author: Yaeta Endo
Publisher: Humana Press
ISBN: 9781603273305
Category : Science
Languages : en
Pages : 0

Book Description
During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.

Cell-Free Protein Production

Cell-Free Protein Production PDF Author: Yaeta Endo
Publisher: Humana Press
ISBN: 9781603273305
Category : Science
Languages : en
Pages : 0

Book Description
During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.

Medical Biosensors for Point of Care (POC) Applications

Medical Biosensors for Point of Care (POC) Applications PDF Author: Roger Narayan
Publisher: Woodhead Publishing
ISBN: 0081000782
Category : Medical
Languages : en
Pages : 318

Book Description
Medical Biosensors for Point of Care (POC) Applications discusses advances in this important and emerging field which has the potential to transform patient diagnosis and care. Part 1 covers the fundamentals of medical biosensors for point-of-care applications. Chapters in part 2 go on to look at materials and fabrication of medical biosensors while the next part looks at different technologies and operational techniques. The final set of chapters provide an overview of the current applications of this technology. Traditionally medical diagnostics have been dependent on sophisticated technologies which only trained professionals were able to operate. Recent research has focused on creating point-of-care diagnostic tools. These biosensors are miniaturised, portable, and are designed to be used at the point-of-care by untrained individuals, providing real-time and remote health monitoring. - Provides essential knowledge for designers and manufacturers of biosensors for point-of-care applications - Provides comprehensive coverage of the fundamentals, materials, technologies, and applications of medical biosensors for point-of-care applications - Includes contributions from leading international researchers with extensive experience in developing medical biosensors - Discusses advances in this important and emerging field which has the potential to transform patient diagnosis and care

Star-Formation Rates of Galaxies

Star-Formation Rates of Galaxies PDF Author: Andreas Zezas
Publisher: Cambridge University Press
ISBN: 1316877523
Category : Science
Languages : en
Pages : 318

Book Description
Star-formation is one of the key processes that shape the current state and evolution of galaxies. This volume provides a comprehensive presentation of the different methods used to measure the intensity of recent or on-going star-forming activity in galaxies, discussing their advantages and complications in detail. It includes a thorough overview of the theoretical underpinnings of star-formation rate indicators, including topics such as stellar evolution and stellar spectra, the stellar initial mass function, and the physical conditions in the interstellar medium. The authors bring together in one place detailed and comparative discussions of traditional and new star-formation rate indicators, star-formation rate measurements in different spatial scales, and comparisons of star-formation rate indicators probing different stellar populations, along with the corresponding theoretical background. This is a useful reference for students and researchers working in the field of extragalactic astrophysics and studying star-formation in local and higher-redshift galaxies.

Sex and Gender Factors Affecting Metabolic Homeostasis, Diabetes and Obesity

Sex and Gender Factors Affecting Metabolic Homeostasis, Diabetes and Obesity PDF Author: Franck Mauvais-Jarvis
Publisher: Springer
ISBN: 3319701789
Category : Medical
Languages : en
Pages : 630

Book Description
The book provides a reference for years to come, written by world-renowned expert investigators studying sex differences, the role of sex hormones, the systems biology of sex, and the genetic contribution of sex chromosomes to metabolic homeostasis and diseases. In this volume, leaders of the pharmaceutical industry present their views on sex-specific drug discovery. Many of the authors presented at the Keystone Symposium on “Sex and gender factors affecting metabolic homeostasis, diabetes and obesity” to be held in March 2017 in Lake Tahoe, CA. This book will generate new knowledge and ideas on the importance of gender biology and medicine from a molecular standpoint to the population level and to provide the methods to study them. It is intended to be a catalyst leading to gender-specific treatments of metabolic diseases. There are fundamental aspects of metabolic homeostasis that are regulated differently in males and females, and influence both the development of diabetes and obesity and the response to pharmacological intervention. Still, most preclinical researchers avoid studying female rodents due to the added complexity of research plans. The consequence is a generation of data that risks being relevant to only half of the population. This is a timely moment to publish a book on sex differences in diseases as NIH leadership has asked scientists to consider sex as a biological variable in preclinical research, to ensure that women get the same benefit of medical research as men.

Salts of Amino Acids

Salts of Amino Acids PDF Author: Michel Fleck
Publisher: Springer
ISBN: 3319062999
Category : Medical
Languages : en
Pages : 581

Book Description
Over the last decades, amino acids have been found to be of importance in many fields of science. Apart from their biological function, this family of organic compounds has been employed in the synthesis of a vast variety of salts, with impact on areas such as materials science, pharmaceutical or physical research. This covers a wide range, from the discovery of important ferroelectrics or non-linear optical materials to nutrients, flavor enhancers or drugs. This book describes amino acids and their salts with cations, anions and inorganic compounds from a chemical, physical and crystallographical point of view. Additional data on structural properties, crystal growth and the relation of structure and physical properties of amino acid salts is discussed.

Clean Energy Materials

Clean Energy Materials PDF Author: Lang Qin
Publisher:
ISBN: 9780841298613
Category : Electric batteries
Languages : en
Pages :

Book Description


Detection of Non-Amplified Genomic DNA

Detection of Non-Amplified Genomic DNA PDF Author: Giuseppe Spoto
Publisher: Springer Science & Business Media
ISBN: 940071226X
Category : Science
Languages : en
Pages : 320

Book Description
This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.

The Hadron Mass Spectrum

The Hadron Mass Spectrum PDF Author: Eberhard Klempt
Publisher:
ISBN:
Category : Hadron spectrocopy
Languages : en
Pages : 464

Book Description


Gauge Field Theories

Gauge Field Theories PDF Author: Stefan Pokorski
Publisher: Cambridge University Press
ISBN: 9780521478168
Category : Science
Languages : en
Pages : 636

Book Description
An expanded and up-dated book examining gauge theories and their symmetries.

Hybrid Phosphor Materials

Hybrid Phosphor Materials PDF Author: Kanchan Upadhyay
Publisher: Springer Nature
ISBN: 3030905063
Category : Technology & Engineering
Languages : en
Pages : 358

Book Description
This book reports the basics of hybrid phosphor materials, their synthesis routes and their special properties and characterization techniques. It gives the reader information about the natural origins and development of hybrid materials, which are developed by combining inorganic and organic species in one material interface-determined materials. The book provides a general classification of hybrid materials, wherein inorganic materials modified by organic moieties are distinguished from organic materials or matrices modified by inorganic constituents. It gives a focus to the functionalization of organic materials by inorganic additives. The application areas covered include optoelectronic field, sensor applications, biological and environmental applications.