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Author: Hohjai Lee Publisher: ISBN: Category : Languages : en Pages : 198
Book Description
Photosynthesis begins with absorbing the sun light by the light harvesting complexes. The solar energy is then funneled into the reaction center (RC) via the energy transfer between the light harvesting complexes at ultrafast rates (̃1/100fs) with extremely high quantum efficiency (̃100 %). Most of the complexes are composed of pigments and protein matrices that tightly bind them. The pigments are responsible for absorbing and transferring the energy. The roles of the protein environment of photosynthetic pigment-protein complexes have been suggested, but the detailed mechanisms are still not fully understood. In this dissertation, non-linear spectroscopic methods using ultrashort pulses (̃ 40-fs FWHM), three-pulse photon echo studies are presented to investigate the roles of protein environment of the photosynthetic bacterial RC. The technique characterizes the protein dynamics around the pigments (a bacteriochlorophyll a, B and a bacteriopheophytin a, H) in the RC. In particular, two-color three-pulse electronic coherence photon echo technique is used to observe the quantum coherence between the excited states of coupled H and B, whose life time is sensitive to the protein dynamics. I found a long-lasting quantum coherence suggesting that the protein actively preserves the quantum coherence. A scenario in which the long-lasting coherence can accelerate the rate of energy trap is described with a simple Bloch model simulation. In addition, one- and two-color three-pulse photon echo peak shift (1C- and 2C3PEPS) techniques are used to measure the coupling strength between H and B in the wild type RC. The coupling strength is facilitated from the geometry between the pigments governed by the protein environment. The simulation based on the standard response function formalism is used to obtain the coupling strength. 2C3PEPS signal from H and B of the oxidized RC is reproduced to extract the coupling constant between them by quantum-master equation which efficiently incorporates pulse overlap effect and bath memory effect. The values will enable the molecular level of studies on the photosynthetic energy and electron transfer.
Author: Hohjai Lee Publisher: ISBN: Category : Languages : en Pages : 198
Book Description
Photosynthesis begins with absorbing the sun light by the light harvesting complexes. The solar energy is then funneled into the reaction center (RC) via the energy transfer between the light harvesting complexes at ultrafast rates (̃1/100fs) with extremely high quantum efficiency (̃100 %). Most of the complexes are composed of pigments and protein matrices that tightly bind them. The pigments are responsible for absorbing and transferring the energy. The roles of the protein environment of photosynthetic pigment-protein complexes have been suggested, but the detailed mechanisms are still not fully understood. In this dissertation, non-linear spectroscopic methods using ultrashort pulses (̃ 40-fs FWHM), three-pulse photon echo studies are presented to investigate the roles of protein environment of the photosynthetic bacterial RC. The technique characterizes the protein dynamics around the pigments (a bacteriochlorophyll a, B and a bacteriopheophytin a, H) in the RC. In particular, two-color three-pulse electronic coherence photon echo technique is used to observe the quantum coherence between the excited states of coupled H and B, whose life time is sensitive to the protein dynamics. I found a long-lasting quantum coherence suggesting that the protein actively preserves the quantum coherence. A scenario in which the long-lasting coherence can accelerate the rate of energy trap is described with a simple Bloch model simulation. In addition, one- and two-color three-pulse photon echo peak shift (1C- and 2C3PEPS) techniques are used to measure the coupling strength between H and B in the wild type RC. The coupling strength is facilitated from the geometry between the pigments governed by the protein environment. The simulation based on the standard response function formalism is used to obtain the coupling strength. 2C3PEPS signal from H and B of the oxidized RC is reproduced to extract the coupling constant between them by quantum-master equation which efficiently incorporates pulse overlap effect and bath memory effect. The values will enable the molecular level of studies on the photosynthetic energy and electron transfer.
Author: Jacques Breton Publisher: Springer Science & Business Media ISBN: 1461530504 Category : Science Languages : en Pages : 418
Book Description
The NATO Advanced Research Workshop entitled "The Photosynthetic Bacterial Reaction Center: Structure, Spectroscopy, and Dynamics" was held May 10-15, 1992, in the Maison d'H6tes of the Centre d'Etudes Nuc1eaires de Cadarache near Aix-en-Provence in the south of France. This workshop is the most recent of a string of meetings which started in Feldafing (Germany) in March 1985, soon after the three-dimensional structure of the bacterial reaction center had been elucidated by X-ray crystallography. This was followed, in September 1987, by a workshop in Cadarache and, in March 1990, by a second meeting in Feldafing. Although one of the most important processes on Earth, photosynthesis is still poorly understood. Stimulated by the breakthrough of solving the bacterial reaction center structure at atomic resolution, the field of relating this structure to the function of the reaction center, i. e. the remarkably efficient conversion and storage of solar energy, has been developing vigorously. Once the general organization of the cofactors and some details of the protein-cofactor interactions were known, it became possible to combine a variety of spectroscopic techniques with the powerful tool of site-directed mutagenesis in order to address increasingly incisive questions about the specific role of some amino acid residues in the electron transfer process. Still another promising tool is being developed, namely the exchange of a number of the native bacteriochlorophyll and bacteriopheophytin cofactors by chemically modified pigments.
Author: Seungwan Ryu Publisher: ISBN: Category : Languages : en Pages : 98
Book Description
Photosynthetic light harvesting begins with collecting ambient photons via excitation of pigment molecules. The sophisticated design of the pigment-protein complexes is based on the cooperative network among structural motifs of pigment molecules and amino acid residues in proteins to achieve the remarkable efficiency and speed in light harvesting. The insight from nature's photosynthetic nano-machinery can be translated to an optimal material design. The principle behind such inherent excitation energy transfer dynamics were revealed with the advance of the femtosecond laser technique. One of the examples is the quantum mechanical coherence excitation energy transfer observed by nonlinear photon echo technique. This dissertation presents the investigation of the bacterial reaction center complexes using electronic coherence photon echo spectroscopy. The study focused on elucidating the origin of the coherence signals and identifying quantum mechanical components relevant to the excitation energy transfer. Experimental variables affecting the coherence signals were explored for the excitation energy transfer of photosynthetic bacterial reaction center. The result showed that the vibrational coherence mimics the behavior of the electronic coherence, and the lifetime is near 2 ps. A mutant bacterial reaction center was also examined to confirm the vibrational contribution. A new polarization control experiment indicates that majority of the observed vibrational coherence is from the electronic ground state.
Author: J. Breton Publisher: Springer Science & Business Media ISBN: 1489908153 Category : Science Languages : en Pages : 433
Book Description
This volume contains the contributions from the speakers at the NATO Advanced Research Workshop on "Structure of the Photosynthetic Bacterial Reaction Center X-ray Crystallography and Optical Spectroscopy with Polarized Light" which was held at the "Maison d'Hotes" of the Centre d'Etudes Nucleaires de Cadarache in the South of France, 20-25 September, 1987. This meeting continued in the spirit of a previous workshop which took place in Feldafing (FRG), March 1985. Photosynthetic reaction centers are intrinsic membrane proteins which, by performing a photoinduced transmembrane charge separation, are responsible for the conversion and storage of solar energy. Since the pioneering work of Reed and Clayton (1968) on the isolation of the reaction center from photosynthetic bacteria, optical spectroscopy with polarized light has been one of the main tools used to investigate the geometrical arrangement of the various chromophores in these systems. The recent elucidation by X-ray crystallography of the structure of several bacterial reaction centers, a breakthrough initiated by Michel and Deisenhofer, has provided us with the atomic coordinates of the pigments and some details about their interactions with neighboring aminoacid residues. This essential step has given a large impetus both to experimentalists and to theoreticians who are now attempting to relate the X-ray structural model to the optical properties of the reaction center and ultimately to its primary biological function.
Author: Thijs Aartsma Publisher: Springer Science & Business Media ISBN: 1402082509 Category : Science Languages : en Pages : 538
Book Description
Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the publication of the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.
Author: Maria-Elisabeth Michel-Beyerle Publisher: Springer Science & Business Media ISBN: 3642611575 Category : Science Languages : en Pages : 418
Book Description
Results of this third Feldafing Meeting can be considered as the harvest of novel techniques in spectroscopy, biochemistry and molecular biology to the bacterial photosynthetic reaction center. New information pertains to the crystallographic and electronic structure as well as to the dynamics of primary events and the role of the protein. The answer to one long-standing problem, the mechanism of primary charge separation, converges towards a sequential scheme, supported by femtosecond spectroscopy on reaction centers with selectively modified energetics.
Author: Hohjai Lee
Author: Hohjai Lee
Author: Ritesh Agarwal
Author: Jacques Breton
Author: Seungwan Ryu
Author: J. Breton
Author: Thijs Aartsma
Author: Maria-Elisabeth Michel-Beyerle
Author: J. Breton
Author: Jacques Breton
Author: