Triple Helix Forming Oligonucleotides PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Triple Helix Forming Oligonucleotides PDF full book. Access full book title Triple Helix Forming Oligonucleotides by Claude Malvy. Download full books in PDF and EPUB format.
Author: Claude Malvy Publisher: Springer Science & Business Media ISBN: 1461551773 Category : Science Languages : en Pages : 296
Book Description
Sequence-specific DNA binding ligands, amongst which triple helix forming oligonucleotides are the most efficient as yet, represent promising tools in a number of fields. One of their most promising applications is as antiviral tools: they can specifically target a viral gene, even if it is integrated into the host genome, and be used to specifically inactivate the viral gene or even destroy the cells harboring this gene. However, from science fiction to science there remains a gap; and we are at the moment on the threshold of this fascinating field. Triple Helix Forming Oligonucleotides considers the different aspects of the design and improvement, current or future, of these molecules and their structural analysis, as well as their applications, with special emphasis on the attempts to obtain biological effects of these potentially important tools. What emerges is that the current state of the research is encouraging, and that these molecules are already useful in some biotechnology applications.
Author: Claude Malvy Publisher: Springer Science & Business Media ISBN: 1461551773 Category : Science Languages : en Pages : 296
Book Description
Sequence-specific DNA binding ligands, amongst which triple helix forming oligonucleotides are the most efficient as yet, represent promising tools in a number of fields. One of their most promising applications is as antiviral tools: they can specifically target a viral gene, even if it is integrated into the host genome, and be used to specifically inactivate the viral gene or even destroy the cells harboring this gene. However, from science fiction to science there remains a gap; and we are at the moment on the threshold of this fascinating field. Triple Helix Forming Oligonucleotides considers the different aspects of the design and improvement, current or future, of these molecules and their structural analysis, as well as their applications, with special emphasis on the attempts to obtain biological effects of these potentially important tools. What emerges is that the current state of the research is encouraging, and that these molecules are already useful in some biotechnology applications.
Author: Publisher: ISBN: Category : Languages : en Pages : 6
Book Description
Triple Helix Design Principles. The first research priority of the program of Navy support was to refine our understanding of triple helix forming oligonucleotides (TFOs). Binding affinity and strand orientation of triplex forming oligonucleotides were measured as a function of base composition. Based upon that work, which was published in Biochemistry (ref. 3), we showed that triple helices containing GGC and TAT triplets were stable at physiological pH and prefer to bind with an antiparallel strand orientation. This study and the accompanying patent application provided the first evidence that TFOs can bind in a site selective fashion at physiological pH and the first explicit evidence for a new (antiparallel) class of triple helix. This work also served as the basis for the filing of a continuation in part to a patent application, filed 12/89. DNA Recognition, Triplet helix formation.
Author: Valery N. Soyfer Publisher: Springer Science & Business Media ISBN: 1461239729 Category : Science Languages : en Pages : 373
Book Description
The ability of DNA to exist in configurations other than its classical double-stranded form has been known for many years. There has been a spectacular recent surge of interest in these forms, notably in the three-stranded or triple-helical form. Triplex-like nucleic acids are now known to exist in vivo, and may well participate in significant biological processes. Interest in triple-helical nucleic acids has been greatly stimulated by their potential exploitation to control gene expression, serve as tools in genome mapping strategies, etc. The authors have written an encyclopedic introduction to nucleic acid triplexes based on many years of familiarity with the topic. The book includes information on chemistry, conformation, physical properties, applications, and hypotheses about the biological role of triplexes. It pays particular attention to the different methods for investigating these molecules, a feature which will be welcomed by those new to the field.
Author: Piet Herdewijn Publisher: Springer Science & Business Media ISBN: 1592598234 Category : Medical Languages : en Pages : 436
Book Description
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Author: Michael J Waring Publisher: Royal Society of Chemistry ISBN: 1788014286 Category : Science Languages : en Pages : 432
Book Description
There have been remarkable advances towards discovering agents that exhibit selectivity and sequence-specificity for DNA, as well as understanding the interactions that underlie its propensity to bind molecules. This progress has important applications in many areas of biotechnology and medicine, notably in cancer treatment as well as in future gene targeting therapies. The editor and contributing authors are leaders in their fields and provide useful perspectives from diverse and interdisciplinary backgrounds on the current status of this broad area. The role played by chemistry is a unifying theme. Early chapters cover methodologies to evaluate DNA-interactive agents and then the book provides examples of DNA-interactive molecules and technologies in development as therapeutic agents. DNA-binding metal complexes, peptide and polyamide–DNA interactions, and gene targeting tools are some of the most compelling topics treated in depth. This book will be a valuable resource for postgraduate students and researchers in chemical biology, biochemistry, structural biology and medicinal fields. It will also be of interest to supramolecular chemists and biophysicists.
Author: Alberte Pullman Publisher: Springer Science & Business Media ISBN: 9780792331025 Category : Science Languages : en Pages : 518
Book Description
Gathering together a number of experts in the world, the 27th Jerusalem Symposium was devoted to the theme of the modelling of biomolecular structures and mechanisms. As a result of recent growth in both importance and audience, the papers contained in this volume present an evaluation of the status of the present knowledge in this field. The main topics covered by this year's Symposium include nucleic acids and their interactions, proteins and their interaction, membranes and their interactions, enzymatic processes and the pharmacological and medical aspects of these subjects. Readers should benefit from the interdisciplinary approach which provides coverage of both theoretical and experimental advances.
Author: Claude Malvy
Author: Claude Malvy
Author: 
Author: Valery N. Soyfer
Author: Cheryl Anne Hobbs
Author: Piet Herdewijn
Author: Kordula Kautz
Author: Zoltán Sándor
Author: Michael J Waring
Author: Alberte Pullman
Author: Eldon Scott Priestley