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Author: Matt Guille Publisher: Springer Science & Business Media ISBN: 1592596789 Category : Science Languages : en Pages : 222
Book Description
The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.
Author: Matt Guille Publisher: Springer Science & Business Media ISBN: 1592596789 Category : Science Languages : en Pages : 222
Book Description
The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.
Author: Publisher: Academic Press ISBN: 0123870372 Category : Technology & Engineering Languages : en Pages : 317
Book Description
This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls Illustrates many techiques in full-color Summarizes the Zebrafish Genome Project
Author: Publisher: Academic Press ISBN: 0128034890 Category : Science Languages : en Pages : 346
Book Description
The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology, is the latest edition in the Methods in Cell Biology series that looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology. Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies Written by experts in the field Contains cutting-edge material on the topic of developmental biology in zebrafish New two part edition of this important volume
Author: Publisher: Academic Press ISBN: 0123848938 Category : Science Languages : en Pages : 363
Book Description
This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls Summarizes the Zebrafish Genome Project
Author: Publisher: Academic Press ISBN: 0128033436 Category : Medical Languages : en Pages : 386
Book Description
Zebrafish at the Interface of Development and Disease Research presents the latest on the shared pathways that govern development and contribute to disease. Zebrafish have traditionally been used to study vertebrate development, providing interesting data on the developmental processes and genes that are implicated in disease. This new release in the series contains informative discussions on congenital heart defects in zebrafish, the use of zebrafish in studying kidney development and disease, and muscle development, homeostasis and disease in zebrafish. Each chapter interweaves the study of zebrafish development and its application to the immune system, the kidney, liver, heart and others. The subject matter is unique - no single volume has pulled together the theme of development and disease with zebrafish as a centerpiece Leading experts in the field - all of them running zebrafish focused labs for each chapter
Author: Publisher: Elsevier ISBN: 0080473458 Category : Science Languages : en Pages : 655
Book Description
This volume of Methods in Cell Biology, the first of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls Illustrates many techiques in full-color Summarizes the Zebrafish Genome Project
Author: Monte Westerfield Publisher: Academic Press ISBN: 0080923437 Category : Science Languages : en Pages : 581
Book Description
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and “tricks of the trade to both experienced researchers and more junior members in the lab. Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error
Author: Publisher: Academic Press ISBN: 0128052066 Category : Science Languages : en Pages : 682
Book Description
The Zebrafish: Cellular and Developmental Biology, Part B Developmental Biology, the second volume on the topic in the Methods in Cell Biology series, looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology. Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies Chapters written by experts in the field Contains cutting-edge material on the topic of zebrafish and developments relating to their cellular and developmental biology New, two part Fourth Edition in this important volume
Author: Zhiyuan Gong Publisher: World Scientific ISBN: 9814482854 Category : Science Languages : en Pages : 684
Book Description
The zebrafish is the most important fish model in developmental and genetic analyses. This book contains 19 review articles covering a broad spectrum of topics, from development to genetic tools. The contents range from early development, the role of maternal factors and gastrulation, to tissue differentiation and organogenesis, such as development of the organizer, notochord, floor plate, nervous system, somites, muscle, skeleton and endoderm. The genetic tools cover morpholino knock-down, transgenics, fish cloning, transposons and genome evolution. The book also includes two chapters on genome mapping and embryonic stem cells in medaka, another important model fish. Summarizing the state-of-the-art studies of the zebrafish model and focusing on the molecular aspects of development, this book is a valuable reference for students learning the basic aspects of the zebrafish model, and for researchers seeking resources in zebrafish research.
Author: Matt Guille
Author: Matt Guille
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Author: Monte Westerfield
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Author: Zhiyuan Gong
Author: Monte Westerfield