The Potential Impact of Pathogens on Honey Bee, Apis Mellifera L., Colonies and Possibilities for Their Control PDF Download

Author: Suresh Desai
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Excessive honey bee colony losses all over the world are believed to be caused by multiple stressors. In this thesis, I characterized and quantified pathogen levels in honey bee colonies, studied their interactions with each other and with their associated parasite vectors, examined factors that influence their combined impacts on honey bees and developed methods to manage honey bee viruses so that colony losses can be minimized. My baseline study of virus prevalence and concentration in healthy and unhealthy (showing visible signs of disease) colonies in Canada showed that seven economically important viruses (DWV, BQCV, IAPV, KBV, SBV, ABPV, and CBPV) were all widely distributed in Canada. Differences in concentration and prevalence of some viruses were found between unhealthy and healthy colonies but these differences may have been due in part to seasonal or regional effects. Studies of the impact of viruses on worker bee populations over winter showed different factors were correlated with bee loss in different environments. Spring concentrations of DWV and mean abundance of Varroa (Varroa destructor) were positively correlated with bee loss and negatively correlated with spring population size in outdoor-wintered colonies. Fall concentration of IAPV was negatively correlated with spring population size of colonies in indoor-wintering environments but not in outdoor-environments. My study showed that it is important to consider location of sampling when associating pathogen loads with bee loss with Nosema and BQCV. Seasonal patterns of parasites and pathogens were characterized for each wintering methods (indoor and outdoor). My results revealed lower ABPV and Nosema ceranae prevalence and lower DWV concentration in genetically diverse than genetically similar colonies. I showed that within colony genetic diversity may be an important evolutionary adaptation to allow honey bees to defend against a wide range of diseases. In laboratory studies, I showed that feeding DWV to larvae in the absence of Varroa causes wing deformity and decreased survival rates of adult bees relative to bees not fed DWV. Finally, I showed that RNA silencing can be used to reduce DWV concentrations in immature and adult bees, reduce wing deformity in emerging adults, and increase their longevity relative to controls.

Author: Tarlochan S. Dhadialla
Publisher: Academic Press
ISBN: 0123915007
Category : Nature
Languages : en
Pages : 564

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Advances in Insect Physiology is committed to publishing volumes containing comprehensive and in-depth reviews on all aspects of insect physiology. First published in 1963, these volumes are an essential reference source for invertebrate physiologists, insect neurobiologists, entomologists, zoologists and insect biochemists. This volume is themed on small RNAs and RNAi in insects. Contains comprehensive and in-depth reviews. Essential reference source for invertebrate physiologists, insect neurobiologists, entomologists, zoologists and insect biochemists. This volume is themed on small RNAs and RNAi in insects.

Author: Richard Odemer
Publisher:
ISBN:
Category : Bees
Languages : en
Pages :

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Author: Carla Mucignat-Caretta
Publisher: CRC Press
ISBN: 1466553413
Category : Medical
Languages : en
Pages : 614

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Intraspecific communication involves the activation of chemoreceptors and subsequent activation of different central areas that coordinate the responses of the entire organism—ranging from behavioral modification to modulation of hormones release. Animals emit intraspecific chemical signals, often referred to as pheromones, to advertise their presence to members of the same species and to regulate interactions aimed at establishing and regulating social and reproductive bonds. In the last two decades, scientists have developed a greater understanding of the neural processing of these chemical signals. Neurobiology of Chemical Communication explores the role of the chemical senses in mediating intraspecific communication. Providing an up-to-date outline of the most recent advances in the field, it presents data from laboratory and wild species, ranging from invertebrates to vertebrates, from insects to humans. The book examines the structure, anatomy, electrophysiology, and molecular biology of pheromones. It discusses how chemical signals work on different mammalian and non-mammalian species and includes chapters on insects, Drosophila, honey bees, amphibians, mice, tigers, and cattle. It also explores the controversial topic of human pheromones. An essential reference for students and researchers in the field of pheromones, this is also an ideal resource for those working on behavioral phenotyping of animal models and persons interested in the biology/ecology of wild and domestic species.

Author: Jennifer M. Weisbrod
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Honey bees (Apis mellifera L.) face high annual declines in the United States and pesticide exposure is a factor. Bees may return with residues from the environment or become exposed through beekeeper-applied compounds, however the effects of pesticide accumulation in combs on bees have not been well-studied. To further examine this, chlorothalonil fungicide and beekeeper-applied acaricide amitraz, common pesticides within the hive, were applied to comb. Queen bees laid eggs onto treated and control combs (acetone solvent or untreated) then larval development and adult worker bee measures (hypopharyngeal gland size and abdominal lipids) were compared to determine potential effects of pesticide residues on bee health. Results indicates that larvae reared in comb treated with amitraz developed significantly smaller hypopharyngeal glands. Exposure to newer chemistries, may not result in rapid losses but rather colonies may exhibit slow chronic losses over time, indicating impacts may be due to persistent residual effects. Here, we assessed the use of dead bee traps for monitoring pesticide incidents. Trap efficacy was assessed by exposing workers imidacloprid (or freeze-killed (control)) and monitoring traps to determine when dead/dying bees are removed from the hive (recapture rates). Dead bee traps recaptured 27.7% of freeze-killed control bees and significantly less of the imidacloprid-treated bees. Trap collection data from three apiaries indicate distinct differences in timing of observed mortality by location. Results elucidate how pesticide exposures may be monitored and this thesis concludes with an instructional guide to build and use traps to better monitor for hive health issues.

Author: Terry Ryan Kane
Publisher: John Wiley & Sons
ISBN: 1119583373
Category : Medical
Languages : de
Pages : 401

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Book Description
An essential guide to the health care of honey bees Honey Bee Medicine for the Veterinary Practitioner offers an authoritative guide to honey bee health and hive management. Designed for veterinarians and other professionals, the book presents information useful for answering commonly asked questions and for facilitating hive examinations. The book covers a wide range of topics including basic husbandry, equipment and safety, anatomy, genetics, the diagnosis and management of disease. It also includes up to date information on Varroa and other bee pests, introduces honey bee pharmacology and toxicology, and addresses native bee ecology. This new resource: Offers a guide to veterinary care of honey bees Provides information on basic husbandry, examination techniques, nutrition, and more Discusses how to successfully handle questions and 'hive calls' Includes helpful photographs, line drawings, tables, and graphs Written for veterinary practitioners, veterinary students, veterinary technicians, scientists, and apiarists, Honey Bee Medicine for the Veterinary Practitioner is a comprehensive and practical book on honey bee health.

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309102898
Category : Technology & Engineering
Languages : en
Pages : 327

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Book Description
Pollinators-insects, birds, bats, and other animals that carry pollen from the male to the female parts of flowers for plant reproduction-are an essential part of natural and agricultural ecosystems throughout North America. For example, most fruit, vegetable, and seed crops and some crops that provide fiber, drugs, and fuel depend on animals for pollination. This report provides evidence for the decline of some pollinator species in North America, including America's most important managed pollinator, the honey bee, as well as some butterflies, bats, and hummingbirds. For most managed and wild pollinator species, however, population trends have not been assessed because populations have not been monitored over time. In addition, for wild species with demonstrated declines, it is often difficult to determine the causes or consequences of their decline. This report outlines priorities for research and monitoring that are needed to improve information on the status of pollinators and establishes a framework for conservation and restoration of pollinator species and communities.

Author: Dylan Alexandra Cleary
Publisher:
ISBN:
Category : Honeybee
Languages : en
Pages : 244

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Book Description
The health and viability of colonies of the honey bee, Apis mellifera, in the United States have fluctuated dramatically over the past decade. This poses a substantial threat to agricultural production in this country. Currently, no single factor has been identified for this decline. Rather, it has been suggested that the interaction between multiple biotic and abiotic stressors may be responsible. Among these factors are pesticides, habitat loss, climate and weather, parasites and pathogens, and colony management techniques. For this reason, it is important to examine the prevalence of honey bee parasite and pathogen infection at the state level in comparison to national survey data. In the research described herein, molecular diagnostics were performed on worker honey bee samples from Arkansas hobbyist beekeepers and Oklahoma migratory beekeepers to detect the presence of the following A. mellifera pathogens and parasites: protozoans Nosema apis and N. ceranae; bacterial pathogens Spiroplasma apis and S. melliferum; Trypanosomatid parasites Crithidia mellificae and Lotmaria passim and the parasitic phorid fly Apocephalus borealis. A study including both migratory honey bee colonies and hobbyist managed colonies provides a more comprehensive distribution of where these parasite and pathogen species are occurring and potentially why they are occurring. The study determined that N. ceranae (H=11.6%, M=27.6), L. passim (H=11.3%, M=1.1%), and V. destructor (H=45.5%, M=17.2%), occur in both hobbyist and migratory managed colonies. Nosema ceranae was more prevalent in the migratory colonies than the hobbyist colonies. Spiroplasma was also detected in the Oklahoma migratory colony samples (8.05%), but not in the Arkansas hobbyist colonies. Both V. destructor and L. passim were more prevalent in the hobbyist managed colonies. This research resulted in the first detection of Lotmaria passim in Arkansas honey bees, as well as the first documented detection of L. passim and S. melliferum in Oklahoma. Apocephalus borealis, C. mellificae, N. apis, and S. apis were not detected in either the migratory nor the hobbyist colonies. This study compares honey bee management practices at the hobbyist and migratory level to better understand how management influences parasite and pathogen spread and abundance. The use of state-level surveys, when examining parasite and pathogen occurrence, allows for a better understanding of how these pests are spreading, as well as how quickly and by what means.

Author: Stephanie Parreira
Publisher:
ISBN:
Category : Fungicides
Languages : en
Pages : 246

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Book Description
Honey bees (Apis mellifera) are responsible for approximately $17 billion in crop production per year in the United States, and are arguably the most important pollinators in the nation. The future of crop pollination and production is threatened by widespread national honey bee colony losses, which have averaged approximately 30% per year over the past decade. Many factors contribute to colony mortality, but the particular impacts of pesticides are still poorly understood. Here, we investigated the impacts of pesticides under conditions that have not been examined in previous research. Our research focused on the effects of an interaction between the neonicotinoid imidacloprid and the fungicide chlorothalonil, and effects of exposure through multiple routes. To understand the potential impacts of pesticide interactions, we exposed whole colonies to imidacloprid, chlorothalonil, or combination of both chemicals through a pollen diet for one month. We found that many of our response variables were unaffected by our treatments, and that outliers influenced the outcome of several analyses. Brood area and prophenoloxidase activity were significantly affected by different treatments when outliers were excluded, although these differences were no longer significant after the multiple comparisons confidence interval adjustment. Similarly, the number of non-pollen foragers returning to the colonies was affected by the interaction between imidacloprid and time, chlorothalonil and time, and both chemicals and time, when outliers were removed. The interactions indicated that seven weeks after the end of the exposure period, both imidacloprid and chlorothalonil reduced the number of non-pollen foragers returning to the colonies. Imidacloprid and chlorothalonil also reduced the number of total foragers returning to the colonies overall. Our results indicate that colonies may be affected by pesticide exposure long after the exposure period, and that bees exposed to pesticides early in life may be detrimentally affected by that exposure at later stages. To determine whether pesticide exposure through multiple routes has a greater effect on bees than single-route exposure, we conducted a laboratory experiment in which we exposed bees to imidacloprid through pollen diet, sugar syrup, or both routes. We found that exposure through sugar syrup increased the midgut proteolytic enzyme activity overall, as well as glucose oxidase activity after four weeks of exposure. Exposure through sugar syrup, as well as exposure through both routes, increased glucose oxidase activity when outliers were included and excluded from the analysis, respectively. Mortality differed significantly between bees exposed to imidacloprid through sugar syrup and those exposed through both matrices, but none of the treatments were significantly different from the control group. We also found that bees in different treatment groups consumed different amounts of sugar syrup and pollen. Our results indicate the importance of conducting laboratory experiments that better reflect field-realistic pesticide exposure by both incorporating effects over a longer period of exposure, and exposure through multiple routes. In summary, our results provide new knowledge and insights on how pesticides impact long-term colony health. Future research must thoroughly examine statistical procedures, outliers, and statistical power, and must also determine interactions between pesticides and pathogens under different conditions, such as different types of pesticide application, honey bee subspecies, nutritional conditions, season, etc. Discerning the variability in results when these conditions vary will provide a fuller understanding of the true impacts of pesticides on colony health.

Author: Chukwuebuka Egbuna
Publisher: Springer Nature
ISBN: 3030729990
Category : Science
Languages : en
Pages : 262

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This book presents comprehensive coverage on the importance of good nutrition in the treatment and management of obesity, cancer and diabetes. Naturally occurring bioactive compounds are ubiquitous in most dietary plants available to humans and provide opportunities for the management of diseases. The text provides information about the major causes of these diseases and their association with nutrition. The text also covers the role of dietary phytochemicals in drug development and their pathways. Later chapters emphasize novel bioactive compounds as anti-diabetic, anti-cancer and anti-obesity agents and describe their mechanisms to regulate cell metabolism. Written by global team of experts, Dietary Phytochemicals: A Source of Novel Bioactive Compounds for the Treatment of Obesity, Cancer and Diabetes describes the potentials of novel phytochemicals, their sources, and underlying mechanism of action. The chapters were drawn systematically and incorporated sequentially to facilitate proper understanding. This book is intended for nutritionists, physicians, medicinal chemists, drug developers in research and development, postgraduate students and scientists in area of nutrition and life sciences.