The Large Maf Transcription Factor Traffic Jam Regulates Collective Cell Migration During Drosophila Oogenesis PDF Download

Author: Felix Gunawan
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: Trupti Panchal
Publisher:
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Category :
Languages : en
Pages :

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Author: Jeffrey David Alls
Publisher: Library and Archives Canada = Bibliothèque et Archives Canada
ISBN: 9780494025468
Category :
Languages : en
Pages : 278

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Traffic jam (tj) encodes a soma-specific Maf transcription factor required for morphogenesis of the gonads in Drosophila melanogaster. I examined the requirement of tj function throughout oogenesis by studying the expression of follicle cell type specific markers in tj mutant cell clones. I also examined the interaction between tj and JAK-Stat pathway genes using a variety of loss- and gain-of-function mutations. As well as regulating the expression of a number of adhesion molecules, TJ appears to be a general requirement in follicle cells for their proper differentiation throughout oogenesis. Depending on when cells lose tj, their subsequent differentiation is blocked or defective. This suggests that like other Mafs, TJ is a regulator of differentiation. Furthermore, my findings suggest that TJ interacts with the JAK-Stat pathway during oogenesis. I have also identified a possible interaction between TJ and the EGF receptor signaling pathway.

Author: Milica Gioia Arandjelovic
Publisher: National Library of Canada = Bibliothèque nationale du Canada
ISBN: 9780612915879
Category :
Languages : en
Pages : 278

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The gene traffic jam (tj) encodes a soma-specific, large Maf transcription factor, required for gonad morphogenesis in Drosophila. My gain-of-function analysis of tj in adult ovaries revealed both, defects in cell specification and cell behaviour. The ectopic expression of tj during oogenesis produced follicles with ectopic polar cells, missing inter-follicular stalks and stalk cells, absent or non-migrating border cells, non-migrating centripetal cells and defective stretch cells. These tj gain-of-function phenotypes mimic the phenotypes found in Jak-Stat signalling pathway mutants. My data suggest that TJ may normally be involved in cell specification, and may interact with the Jak-Stat signalling pathway. It was also found that two of the mouse homologues of tj, MafB and c-Maf, are expressed in somatic tissues of adult mouse gonads, suggesting that the role of tj in gonad development and gametogenesis may be evolutionarily conserved.

Author: Benjamin David Levine
Publisher:
ISBN:
Category : Cell migration
Languages : en
Pages : 232

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In the follicle cell (FC) epithelium that surrounds the Drosophila egg, a complex set of cell signals specifies two fates that pattern the eggshell: the anterior centripetal FCs and the posterior columnar FCs. It has been shown previously that the long-range morphogen DPP represses the expression of the bunched (bun) gene in the centripetal FCs. Bun, in turn represses anterior gene expression and antagonizes Notch signaling to restrict centripetal FC fates in posterior cells. At stage 9, expression of the centripetal FC-associated gene slbo overlaps bun in anterior FCs; by stage 10A they repress each other's expression to establish a sharp slbo/bun expression boundary. The precises position of the slbo/bun expression boundary is sensitive to Notch signaling, which is required for both slbo activation and bun repression. As centripetal migration proceeds from stages 10B-14. slbo represses its own expression and both slbo loss-of-function mutations and overexpression approaches reveal that slbo is required to coordinate centripetal migration with nurse cell dumping. The expression of the transcription factor cut increases in the centripetal FCs at stage 10B in a pattern complementary to slbo. The pattern of cut expression results through slbo and cut cross-repression. Though Notch signaling represses cut expression prior to stage 7, Notch activation of slbo at 10A does not apparently influence the timing of cut expression at 10B. Notably, FLP-out clones expressing high levels of CUT anywhere in the columnar FCs are associated with increased accumulation of adherens junction proteins and noticeable apical constrictions so that cell shapes in CUT-expressing clones phenotypically resemble stage 10B centripetal FCs. Consistent with the notion that CUT is a centripetal FC selector gene, both cut mutant clones and FLP-out clones expressing slbo recovered the columnar FCs result in aberrant localization of apical adherens junction proteins to the basolateral locations. I propose that bun links long range morphogen signals to cell-contact dependent Notch signaling to produce a sharp columnar/centripetal cell fate boundary and that cut and slbo levels tightly regulate adherens junction polarity required for centripetal migration.

Author: Jose A. Campos-Ortega
Publisher: Springer Science & Business Media
ISBN: 3662024543
Category : Science
Languages : en
Pages : 237

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" . . . but our knowledge is so weak that no philosoph er will ever be able to completely explore the nature of even a fly . . . " * Thornas Aquinas "In Syrnbolurn Apostolorum" 079 RSV p/96 This is a monograph on embryogenesis of the fruit fly Drosophi la melanogaster conceived as a reference book on morphology of embryonie development. A monograph of this extent and con tent is not yet available in the literature of Drosophila embryolo gy, and we believe that there is areal need for it. Thanks to the progress achieved during the last ten years in the fields of devel opmental and molecular genetics, work on Drosophila develop ment has considerably expanded creating an even greater need for the information that we present here. Our own interest for wildtype embryonie development arose several years ago, when we began to study the development of mutants. While those studies were going on we repeatedly had occasion to state in sufficiencies in the existing literature about the embryology of the wildtype, so that we undertook investigating many of these problems by ourselves. Convinced that several of our colleagues will have encountered similar difficulties we decided to publish the present monograph. Although not expressely recorded, Thomas Aquinas probably referred to the domestic fly and not to the fruit fly. Irrespective of which fly he meant, however, we know that Thomas was right in any case.

Author: Diana P. Bratu
Publisher: Humana
ISBN: 9781493928507
Category : Science
Languages : en
Pages : 0

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This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author: Institute of Medicine
Publisher: National Academies Press
ISBN: 0309290651
Category : Medical
Languages : en
Pages : 436

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Individually and collectively, resident microbes play important roles in host health and survival. Shaping and shaped by their host environments, these microorganisms form intricate communities that are in a state of dynamic equilibrium. This ecologic and dynamic view of host-microbe interactions is rapidly redefining our view of health and disease. It is now accepted that the vast majority of microbes are, for the most part, not intrinsically harmful, but rather become established as persistent, co-adapted colonists in equilibrium with their environment, providing useful goods and services to their hosts while deriving benefits from these host associations. Disruption of such alliances may have consequences for host health, and investigations in a wide variety of organisms have begun to illuminate the complex and dynamic network of interaction - across the spectrum of hosts, microbes, and environmental niches - that influence the formation, function, and stability of host-associated microbial communities. Microbial Ecology in States of Health and Disease is the summary of a workshop convened by the Institute of Medicine's Forum on Microbial Threats in March 2013 to explore the scientific and therapeutic implications of microbial ecology in states of health and disease. Participants explored host-microbe interactions in humans, animals, and plants; emerging insights into how microbes may influence the development and maintenance of states of health and disease; the effects of environmental change(s) on the formation, function, and stability of microbial communities; and research challenges and opportunities for this emerging field of inquiry.

Author: Alexander M. Vaiserman
Publisher: Springer
ISBN: 3319183265
Category : Medical
Languages : en
Pages : 351

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​This book looks at aging through research on Drosophila, the fruit fly that is one of the most widely used model organisms in bio gerontology. Work in model organisms can expand the theoretical knowledge of aging: it yields valuable insight into the molecular and cellular processes that underlie aging process, and it can perhaps provide new therapeutic targets for the treatment of age-related disorders in humans. Drosophila models have been developed for a large variety of aging-related processes and diseases, and this book provides readers with an overview of current research on the use of the Drosophila model to understand the genetic, molecular and physiological mechanisms that underlie the aging process. Themes of health span, life extension and longevity-associated genes emerge in this collation of international research on Drosophila that is of relevance to geriatrics and gerontology, animal genetics and genomics, and biomedicine. This fascinating, illustrated book will be of interest to a wide audience, ranging from academic researchers to the general reader.

Author: May Loo
Publisher: Elsevier Health Sciences
ISBN: 1416022996
Category : Health & Fitness
Languages : en
Pages : 533

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"Many parents today are turning to or seeking information about Complementary and Alternative Medicine (CAM) for their children. Whether you initiate alternative therapies or simply need to respond when asked for information or advice, it's crucial to have the most recent, evidence-based information about alternative therapies and know how to safely and effectively integrate them with conventional treatment. This innovative and reliable reference is the ideal resource to have at hand." "This book includes a wide range of complementary and alternative therapies, focusing on those most often used with children: mind/body approaches, manual therapies, lifestyle approaches, alternative systems, energy medicine, and biological agents. Within these main categories, therapies such as acupuncture, chiropractic, massage, homeopathy, herbs, and magnets are covered." "Fifty-five common pediatric conditions are comprehensively discussed, with diagnostic and evidence-based treatment information, followed by authoritative information on the major CAM therapies available for treatment of the condition. Whenever possible, an integrative approach that combines conventional and alternative approaches is presented."--BOOK JACKET.