Author: Lars J. BrandénPublisher:
ISBN: 9789162848699
Category :
Languages : en
Pages : 43
Book Description
The Development of Synthetic Gene Delivery Systems
Author: Lars J. BrandénPublisher:
ISBN: 9789162848699
Category :
Languages : en
Pages : 43
Book Description
The Science and Applications of Synthetic and Systems Biology
Author: Institute of MedicinePublisher: National Academies Press
ISBN: 0309219396
Category : Science
Languages : en
Pages : 570
Book Description
Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.
Biophysical Studies of Synthetic Gene Delivery Systems
Author: Li Kim LeeBiodefense in the Age of Synthetic Biology
Author: National Academies of Sciences, Engineering, and MedicinePublisher: National Academies Press
ISBN: 0309465184
Category : Technology & Engineering
Languages : en
Pages : 189
Book Description
Scientific advances over the past several decades have accelerated the ability to engineer existing organisms and to potentially create novel ones not found in nature. Synthetic biology, which collectively refers to concepts, approaches, and tools that enable the modification or creation of biological organisms, is being pursued overwhelmingly for beneficial purposes ranging from reducing the burden of disease to improving agricultural yields to remediating pollution. Although the contributions synthetic biology can make in these and other areas hold great promise, it is also possible to imagine malicious uses that could threaten U.S. citizens and military personnel. Making informed decisions about how to address such concerns requires a realistic assessment of the capabilities that could be misused. Biodefense in the Age of Synthetic Biology explores and envisions potential misuses of synthetic biology. This report develops a framework to guide an assessment of the security concerns related to advances in synthetic biology, assesses the levels of concern warranted for such advances, and identifies options that could help mitigate those concerns.
Pharmaceutical Gene Delivery Systems
Author: Alain RollandPublisher: CRC Press
ISBN: 9780824742355
Category : Medical
Languages : en
Pages : 448
Book Description
This volume examines the advantages and limitations of the major gene delivery systems and offers guidelines to select the most appropriate viral or synthetic delivery system for specific therapeutic applications. It discusses advances in the design, optimization, and adaptation of gene delivery systems for the treatment of cancerous, cardiovascular, pulmonary, genetic, and infectious diseases.
Development of a Synthetic UPAR Targeted Gene Delivery System
Author: Thomas KeanArtificial Self-assembling Systems for Gene Delivery
Author: Philip L. FelgnerPublisher:
ISBN:
Category : Language Arts & Disciplines
Languages : en
Pages : 216
Book Description
Discusses recent advances in artificial self-assembling systems, including retrotransposon vectors, hairpin ribozymes, triple-helix-forming oligonucleotides, liposome-mediated transfection, and photonic nanostructures. Addresses developing efficient synthetic vectors such as modular self-assembling systems mimicking important features of viral vectors. Presents new developments in synthetic self-assembling gene delivery systems, including innovations in nonviral systems, targeting nucleic acids, ligand-polylysine mediated transfer, dendrimer-mediated transfection, cationic liposomes, and polylysine DNA complexes.
Synthetic Polymer and Microbubble Systems for Gene Delivery to the Brain
Author: James-Kevin Young TanPublisher:
ISBN:
Category :
Languages : en
Pages : 222
Book Description
The natural neurogenesis after neuron death in cases like traumatic brain injury and neurological diseases is sparse. Increasing the replenishment of functional neurons can improve patient outcome and prognosis. One way to encourage neurogenesis is by selective differentiation of neural progenitor cells and neural stem cells in the brain. Gene delivery has emerged as a promising strategy to accomplish this due to the development of effective gene transfer vehicles and the identification of factors that can modulate neural differentiation. The first half of this thesis focuses on improving the efficacy of synthetic gene delivery vehicles. Typically, negative-charged DNA is condensed and protected from degradation by complexing it with positively charged materials. These cationic materials improve gene transfection efficiency by enabling for endosomal uptake and escape from the lysosomal degradation pathway. However, the cationic nature of the materials also brings about deleterious effects such as cell cytotoxicity. Therefore, a there is a balance between transfection efficiency and cytotoxicity with cationic vectors. Strategies to reduce cytotoxicity and improve upon transfection efficiency are explored in this section. Even with the most effective transfection agents, a major hurdle is reaching the neural progenitor cells and neural stem cells in the subventricular zone of the brain. The blood-brain barrier and the blood-cerebrospinal fluid barrier effectively block substances from reaching the brain parenchyma after systemic and intraventricular injection. Recently, the use of microbubbles and ultrasound have been shown to transiently disrupt the blood-brain barrier and allow the permeation of larger molecular drugs into the brain parenchyma. Microbubbles and ultrasound will be investigated as a way to disrupt the brain-cerebrospinal fluid barrier and increase the permeation of our gene delivery vehicles into the brain in the second half of this thesis.
Synthetic DNA Delivery Systems
Author: Dan LuoPublisher: Springer Science & Business Media
ISBN: 9780306477010
Category : Science
Languages : en
Pages : 160
Book Description
DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.
Advanced Textbook On Gene Transfer, Gene Therapy And Genetic Pharmacology: Principles, Delivery And Pharmacological And Biomedical Applications Of Nucleotide-based Therapies (Second Edition)
Author: Daniel SchermanPublisher: World Scientific
ISBN: 1786346893
Category : Science
Languages : en
Pages : 634
Book Description
This unique advanced textbook provides a clear and comprehensive overview of gene delivery, gene therapy and genetic pharmacology, with descriptions of the main gene transfer vectors and a set of selected therapeutic applications, along with safety considerations. The second edition features new groundbreaking material on genome editing using the recently discovered CRISPR/Cas9 system and on cancer immunotherapy by CAR-T cells. It also presents the historical milestone of gene therapy application in the field of severe combined immunodeficiency, and other fields of gene therapy and molecular medicine.The use of gene transfer is exponentially growing in the scientific and medical communities for day-to-day cell biology experiments and swift development of gene therapy, which is already revolutionizing medicine. In this advanced textbook, more than 30 leading scientists come together to explore these topics.This educational introduction provides the background material needed to further explore the subject as well as relevant research literature. It is an invaluable resource to Master, PhD or MD students, post-doctoral scientists or medical doctors, as well as any scientist wishing to deliver a gene or synthetic nucleotide or develop a gene therapy strategy. The second edition's simple and synthetic content will be of value to any reader interested in the biological and medical revolution derived from the elucidation of the human genome.