Temporal and Spatial Regulation of Plant Genes PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Temporal and Spatial Regulation of Plant Genes PDF full book. Access full book title Temporal and Spatial Regulation of Plant Genes by Desh Pal S Verma. Download full books in PDF and EPUB format.
Author: Desh Pal S Verma Publisher: Springer Science & Business Media ISBN: 370916950X Category : Science Languages : en Pages : 347
Book Description
First attempts to isolate plant genes were for those genes that are abun dantly expressed in a particular plant organ at a specific stage of devel opment. However, many important gene products are produced in a very minute quantity and in specialized cell types. Such genes can now be isolated using a variety of approaches, some of which are described in this volume. The rapid progress during the last decade in regeneration of a number of crop plants and the availability of molecular tools to introduce foreign genes in plants is allowing the engineering of specific traits of agri cultural importance. These genes must, however, be regulated in a spatial and temporal manner in order to have desired effects on plant devel opment and productivity. The habitat of plants necessitate adaptive responses with respect to the environmental changes. Starting from germination of the seed, the plant begins to sense environmental cues such as moisture, light, temperature and the presence of pathogens, and begins to respond to them. Little is known about various signal transduction pathways that lead to biochemical and morphogenetic responses, in particular, transition from vegetative to reproductive phase. With the availability of tools to generate specific mutations via transposon tagging, identification and isolation of genes affecting these processes may be facilitated. Transfer of these genes into heterologous environments will allow understanding of the complex processes that control plant development.
Author: Desh Pal S Verma Publisher: Springer Science & Business Media ISBN: 370916950X Category : Science Languages : en Pages : 347
Book Description
First attempts to isolate plant genes were for those genes that are abun dantly expressed in a particular plant organ at a specific stage of devel opment. However, many important gene products are produced in a very minute quantity and in specialized cell types. Such genes can now be isolated using a variety of approaches, some of which are described in this volume. The rapid progress during the last decade in regeneration of a number of crop plants and the availability of molecular tools to introduce foreign genes in plants is allowing the engineering of specific traits of agri cultural importance. These genes must, however, be regulated in a spatial and temporal manner in order to have desired effects on plant devel opment and productivity. The habitat of plants necessitate adaptive responses with respect to the environmental changes. Starting from germination of the seed, the plant begins to sense environmental cues such as moisture, light, temperature and the presence of pathogens, and begins to respond to them. Little is known about various signal transduction pathways that lead to biochemical and morphogenetic responses, in particular, transition from vegetative to reproductive phase. With the availability of tools to generate specific mutations via transposon tagging, identification and isolation of genes affecting these processes may be facilitated. Transfer of these genes into heterologous environments will allow understanding of the complex processes that control plant development.
Author: Ying Li Publisher: ISBN: Category : Languages : en Pages :
Book Description
The dicotyledon seedling undergoes organ-specific photomorphogenic development when exposed to light. The cotyledons open and expand, the apical hook opens and the hypocotyl ceases to elongate. Using the large and easily dissected seedlings of soybean (Glycine max cv. Williams 82), we show that genes involved in photosynthesis and its regulation dominate transcripts specific to the cotyledon, even in etiolated seedlings. Genes for cell wall biosynthesis and metabolism are expressed at higher levels in the hypocotyl, while examination of genes expressed at higher levels in the hook region reveals genes involved in cell division and protein turnover. The early transcriptional events in these three organs in response to a one-hour treatment of far-red light are highly distinctive. Not only are different regulatory genes rapidly regulated by light in each organ, but the early-responsive genes in each organ contain a distinctive subset of known light-responsive cis-regulatory elements. We detected specific light induced gene expression for the root phototropism gene RPT2 in the apical hook, and also phenotypes in Arabidopsis rpt2 mutants demonstrating that the gene is necessary for normal photomorphogenesis in the seedling apex. Significantly, expression of the RPT2promoter fused to a GUS reporter gene shows differential expression across the hook region. We also detect higher expression of AGO1 in the apical hook, a key player in small RNA regulatory pathway, and show that Arabidopsis ago1 mutants display an impaired photomorphogenesis in the seedling apex. Using next-gen sequencing, we show that many miRNAs are responsive to a one-hour treatment of far-red light. Most interestingly, we find that miR166 is specifically induced by one-hour far-red light in the convex side of apical hook. Overall, we conclude that organ-specific, light-responsive transcriptional networks are active early in photomorphogenesis in the aerial parts of dicotyledon seedlings. Another topic addressed in this thesis is heterosis. Heterosis, also known as hybrid vigor, refers to the phenomenon wherein a F1 hybrid produced from crossing two cultivars of the same species or two different species displays superior phenotypes compared to the inbred parents. Despite its practical applications and scientific importance, the molecular mechanism underlying heterosis is not completely understood. In recent decades, knowledge of the regulatory roles of small RNAs has greatly improved our understanding of many basic biological questions. We therefore applied a global small RNA profiling-by-sequencing approach to characterize the inheritance of small RNA expression patterns in Arabidopsis reciprocal hybrids. Two Arabidopsis thaliana accessions, Columbia and Landsberg erecta, were crossed reciprocally to produce hybrids. The small RNA expression patterns of both parents and two hybrids were compared. We find that the most common expression patterns for small RNAs in hybrid Arabidopsis are dominance for the parent with lower relative expression and additive between the parental expression. Arabidopsis Analysis of the genomic origin of the differentially expressed small RNAs suggested that they are mostly 24nt siRNA associated with maintaining genome stability and gene regulation. Interestingly, the transposon associated siRNAs are mostly additively inherited, whereas the gene-associated siRNAs are mainly down-regulated in hybrids. Overall, hybridization in Arabidopsis primarily leads to either no change or a reduction in the relative expression of siRNAs compared to their parents.
Author: Alain Goossens Publisher: Humana ISBN: 9781493960132 Category : Science Languages : en Pages : 0
Book Description
It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.
Author: Dorothee Staiger Publisher: Humana ISBN: 9781493945870 Category : Science Languages : en Pages : 0
Book Description
Plant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein interaction, cellular redox state, Ca2+ levels, and innate immune responses. Furthermore, the use of bioinformatics resources is described to evaluate high throughput data sets and to integrate the data into an overarching picture of circadian networks in the cell. Additional chapters focus on seasonal processes like flowering time control, and techniques on trees, moss and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circadian Networks: Methods and Protocols is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.
Author: Lutz Nover Publisher: Springer Science & Business Media ISBN: 3540480374 Category : Science Languages : en Pages : 279
Book Description
The control of plant gene expression at the transcriptional level is the main subject of this volume. Genetics, molecular biology and gene technology have dramatically improved our knowledge of this event. The functional analysis of promoters and transcription factors provides more and more insights into the molecular anatomy of initiation complexes assembled from RNA polymerase and the multiplicity of helper and control proteins. Formation of specific DNA-protein complexes - activating or repressing transcription - is the crux of developmental or environmental control of gene expression. The book presents an up-to-date, critical overview of this rapidly advancing field.
Author: Sacha Baginsky Publisher: Springer Science & Business Media ISBN: 376437439X Category : Science Languages : en Pages : 362
Book Description
This volume aims to provide a timely view of the state-of-the-art in systems biology. The editors take the opportunity to define systems biology as they and the contributing authors see it, and this will lay the groundwork for future studies. The volume is well-suited to both students and researchers interested in the methods of systems biology. Although the focus is on plant systems biology, the proposed material could be suitably applied to any organism.
Author: Desh Pal S Verma
Author: Desh Pal S Verma
Author: Ying Li
Author: Jing-Feng Huang
Author: Xiaoli Ma
Author: Anhthu Quang Bui
Author: Huiqing Wang
Author: Alain Goossens
Author: Dorothee Staiger
Author: Lutz Nover
Author: Sacha Baginsky