Synthesis, Characterization, and Optical Properties of Colloidal Organic Dye-containing Fluorescent Core-shell Silica Nanoparticles PDF Download
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Author: Erik Herz Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
Since fluorescence was first observed in a solution containing quinine sulfate in 1845, methods employing fluorescent materials have abounded. Today, fluorescent probes are widely used in many fields, including biological imaging and security. For all applications brightness, stability, size control and compatibility are key parameters for probe design. Recent research to address these parameters has been focused on synthesizing nanoparticle materials. A class of those materials, core-shell silica nanoparticles, covalently encapsulating organic dyes within the core, have been found to be particularly effective in meeting the need for brighter, more stable probes, while also providing size and compatibility control. This dissertation will describe how I have built on previous research by focusing on the synthesis, characterization and optical properties of core-shell silica nanoparticles that span the full spectral range, from ultraviolet to near-infrared emission. Through scattering corrected comparisons of coumarin dye-containing nanoparticles and dye in aqueous solution, relative quantum yield enhancements of an order of magnitude were measured. Similar brightness enhancements were observed when large Stokes-shift dyes were incorporated into the particles. By creating a graded series of Stokes-shifted particles, single-excitation functionality was added to the fluorescent core-shell silica nanoparticle platform, to allow for spectral multiplexing. Since both light scattering and autofluorescence backgrounds in biological samples tend to be stronger at shorter wavelengths, new probe design has swiftly moved toward near-infrared (NIR) excitation and emission. To this end, NIR fluorescent silica nanoparticles with emissions up to 800nm were synthesized and dye structure-optical property correlations were investigated. Using a self-built fluorescence correlation spectroscopy (FCS) instrument, cyanine dye containing particles of the NIR series were investigated in further depth. The FCS allows for measurement of the size, brightness and concentration of the particles in solution and, with small modifications to the beam-path also provides information on the fluorescence stability of the particles in solution, which is of particular importance in NIR probes. Photostability in aqueous solution was investigated for the NIR dye Cy5 and compared to the dye incorporated into particles, leading to findings that will impact future probe designs.
Author: Erik Herz Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
Since fluorescence was first observed in a solution containing quinine sulfate in 1845, methods employing fluorescent materials have abounded. Today, fluorescent probes are widely used in many fields, including biological imaging and security. For all applications brightness, stability, size control and compatibility are key parameters for probe design. Recent research to address these parameters has been focused on synthesizing nanoparticle materials. A class of those materials, core-shell silica nanoparticles, covalently encapsulating organic dyes within the core, have been found to be particularly effective in meeting the need for brighter, more stable probes, while also providing size and compatibility control. This dissertation will describe how I have built on previous research by focusing on the synthesis, characterization and optical properties of core-shell silica nanoparticles that span the full spectral range, from ultraviolet to near-infrared emission. Through scattering corrected comparisons of coumarin dye-containing nanoparticles and dye in aqueous solution, relative quantum yield enhancements of an order of magnitude were measured. Similar brightness enhancements were observed when large Stokes-shift dyes were incorporated into the particles. By creating a graded series of Stokes-shifted particles, single-excitation functionality was added to the fluorescent core-shell silica nanoparticle platform, to allow for spectral multiplexing. Since both light scattering and autofluorescence backgrounds in biological samples tend to be stronger at shorter wavelengths, new probe design has swiftly moved toward near-infrared (NIR) excitation and emission. To this end, NIR fluorescent silica nanoparticles with emissions up to 800nm were synthesized and dye structure-optical property correlations were investigated. Using a self-built fluorescence correlation spectroscopy (FCS) instrument, cyanine dye containing particles of the NIR series were investigated in further depth. The FCS allows for measurement of the size, brightness and concentration of the particles in solution and, with small modifications to the beam-path also provides information on the fluorescence stability of the particles in solution, which is of particular importance in NIR probes. Photostability in aqueous solution was investigated for the NIR dye Cy5 and compared to the dye incorporated into particles, leading to findings that will impact future probe designs.
Author: Andrew Arthur Burns Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
Nano-sized fluorescent materials have demonstrated great potential in the emergent fields of nanobiotechnology and nanomedicine. To create effective probes for these applications, high brightness, photostability and size control are key parameters for particle design. Core-shell silica nanoparticles incorporating covalently bound dyes in the particle core have been shown to exhibit significant enhancements in dye brightness and photostability while allowing independent control of particle size, color and surface chemistry. Building on this platform, the first part of this dissertation explores a family of quantitative chemical sensors based on the incorporation of analyte-sensitive dyes into the particle shell. By co-localizing sensor and reference dye molecules onto a single particle, local analyte concentration changes can be discerned from local sensor concentration variations by comparison of the sensor and reference intensities facilitating quantitative "ratiometric" chemical imaging. The core-shell architecture is ideal for this application because it sequesters the reference dye in the particle core, while allowing analyte interactions with the sensor dyes near the particle surface. Traditional Stöber-derived sol-gel routes as well as reverse micelle-based methods were employed to generate pH- and Ca+2-sensitive nanoparticles. Specific sensor architectures were applied to intracellular as well as large-scale functional volumetric imaging of intact biofilms and their metabolic activities. To fully realize the potential of fluorescent nanomaterials for biomedical applications, one of the key obstacles is clearance from the body. The second part of this dissertation investigates several of the key parameters for effective clearance (size and surface chemistry) and highlights the development of a series of particles capable of rapid and efficient bodily clearance and demonstrates their efficacy in mice as a model system.
Author: Sung-Hoon Kim Publisher: Elsevier ISBN: 0080465625 Category : Science Languages : en Pages : 283
Book Description
In the last 10 years organic dyes, traditionally used for coloring textiles and other materials, have become increasingly important in the hi-tech industries of electronics and optoelectronics. They can be used in optical data storage, new solar cells and biomedical sensors.Functional Dyes discusses the synthesis of these new, high-value dyes and pigments as well as their applications and performance. The chapters are arranged so that the reader logically advances from the fundamental concepts to more practical aspects of the technology in which they are used.In providing the reader with current information on functional dye chemistry, as well as important developments within the field, Functional Dyes is a valuable information source for dye and material chemists, researchers and graduates, who want a summary of the key advances in the field over the last 10 years and an authoritative view on future developments. * Provides a broad introduction to the science technology of the functional dye application* Reviews recent advances on synthesis and characteristics of the functional dyes and their applications* Is a valuable information source for dye and material chemists and researchers
Author: Pekka Hänninen Publisher: Springer Science & Business Media ISBN: 3642210236 Category : Science Languages : en Pages : 392
Book Description
Lanthanides have fascinated scientists for more than two centuries now, and since efficient separation techniques were established roughly 50 years ago, they have increasingly found their way into industrial exploitation and our everyday lives. Numerous applications are based on their unique luminescent properties, which are highlighted in this volume. It presents established knowledge about the photophysical basics, relevant lanthanide probes or materials, and describes instrumentation-related aspects including chemical and physical sensors. The uses of lanthanides in bioanalysis and medicine are outlined, such as assays for in vitro diagnostics and research. All chapters were compiled by renowned scientists with a broad audience in mind, providing both beginners in the field and advanced researchers with comprehensive information on on the given subject.
Author: Greg T. Hermanson Publisher: Academic Press ISBN: 0080568726 Category : Science Languages : en Pages : 1233
Book Description
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Author: Paul S Clegg Publisher: Royal Society of Chemistry ISBN: 1788015207 Category : Science Languages : en Pages : 266
Book Description
Bicontinuous interfacially jammed emulsion gels, now commonly termed 'bijels', are a class of soft materials, in which interpenetrating, continuous domains of two immiscible fluids are maintained in a rigid arrangement by a jammed layer of colloidal particles at their interface. Such gels have unusual material properties that promise exciting applications across diverse fields from energy materials and catalysis, to food science. This is the first book on the subject and provides the reader with a fundamental introduction. Edited by a recognised authority on bijels, the reader will learn about the bijel and its formation. Bringing together current understanding, this book aims to bring the potential application of bijels to diverse materials challenges closer to fruition. This is a must-have resource for anyone working in soft matter and applied fields.
Author: Thomas Courtney Gardinier (II) Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
In contrast to small molar mass compounds and organic macromolecules, detailed structural investigation of inorganic-organic core-shell nanoparticles remains a challenge. I developed high performance liquid chromatography (HPLC) methods to probe the surface chemistry of Cornell Prime dots (C’ dots), a class of ultrasmall (
Author: American Chemical Society. Meeting Publisher: ISBN: Category : Language Arts & Disciplines Languages : en Pages : 474
Book Description
The book summarizes recent advances in methods to synthesize, stabilize, passivate and functionalize diverse nanoparticles from metals, metal oxides, semiconductors, polymers, organics and biomolecules. A wide range of potential appplications with nanoparticles as building blocks are described.
Author: Bernard Valeur Publisher: Springer Science & Business Media ISBN: 364256853X Category : Science Languages : en Pages : 496
Book Description
This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Author: Erik Herz
Author: Erik Herz
Author: Andrew Arthur Burns
Author: Gerasimos Konstantatos
Author: Sung-Hoon Kim
Author: Pekka Hänninen
Author: Greg T. Hermanson
Author: Paul S Clegg
Author: Thomas Courtney Gardinier (II)
Author: American Chemical Society. Meeting
Author: Bernard Valeur