Author: David Charles GrantPublisher:
ISBN:
Category : Matrix-assisted laser desorption-ionization
Languages : en
Pages : 326
Book Description
Surfactant-mediated Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry in the Analysis of Small Biological Molecules
Author: David Charles GrantPublisher:
ISBN:
Category : Matrix-assisted laser desorption-ionization
Languages : en
Pages : 326
Book Description
Application of Matrix-assisted Laser Desorption/ionization to On-line Aerosol Time-of-flight Mass Spectrometry
Author: Ch.E. KientzFundamental Studies of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS) and Its Applications to the Analysis of Biological Materials
Author: Yongdong ZhuMatrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry for Small Molecule Analysis in Foods
Author: Suzanne Lorraine FrisonPublisher:
ISBN:
Category : Food
Languages : en
Pages : 226
Book Description
Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry
Author: Rainer CramerPublisher: Springer
ISBN: 3319048198
Category : Science
Languages : en
Pages : 287
Book Description
This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics
Laser Desorption/ionization Mass Spectrometry for Direct Profiling and Imaging of Small Molecules from Raw Biological Materials
Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 201
Book Description
Matrix-assisted laser desorption/ionization(MALDI) mass spectrometry(MS) has been widely used for analysis of biological molecules, especially macromolecules such as proteins. However, MALDI MS has a problem in small molecule (less than 1 kDa) analysis because of the signal saturation by organic matrixes in the low mass region. In imaging MS (IMS), inhomogeneous surface formation due to the co-crystallization process by organic MALDI matrixes limits the spatial resolution of the mass spectral image. Therefore, to make laser desorption/ionization (LDI) MS more suitable for mass spectral profiling and imaging of small molecules directly from raw biological tissues, LDI MS protocols with various alternative assisting materials were developed and applied to many biological systems of interest. Colloidal graphite was used as a matrix for IMS of small molecules for the first time and methodologies for analyses of small metabolites in rat brain tissues, fruits, and plant tissues were developed. With rat brain tissues, the signal enhancement for cerebroside species by colloidal graphite was observed and images of cerebrosides were successfully generated by IMS. In addition, separation of isobaric lipid ions was performed by imaging tandem MS. Directly from Arabidopsis flowers, flavonoids were successfully profiled and heterogeneous distribution of flavonoids in petals was observed for the first time by graphite-assisted LDI(GALDI) IMS.
Application of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry to the Study of Bacterial Biomolecules
Author: Teresa Catherine CainPublisher:
ISBN:
Category :
Languages : en
Pages : 234
Book Description
Matrix-assisted Laser Desorption/ionization Mass Spectrometric Study of Biologic Molecules
Author: Yan-Hui LiuMatrix-assisted Laser Desorption Fourier Transform Mass Spectrometry for Biological Compounds
Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 9
Book Description
The recent development of matrix-assisted UV laser desorption (LD) mass spectrometry has made possible the ionization and detection of extremely large molecules (with molecular weights exceeding 100,000 Daltons). This technique has generated enormous interest in the biological community for the direct examination of large peptides and oligonucleotides. Although this matrix-assisted ionization method has been developed and used almost exclusively with time-of-flight (TOF) mass spectrometers, research is currently in progress to demonstrate this technique with trapped ion mass spectrometers, such as Fourier transform ion cyclotron resonance mass spectrometry (FTMS). The potential capabilities of FTMS for wide mass range, high resolution measurement, and ion trapping experiments suggest that this instrumental technique should be useful for the detailed structural characterization of large ions generated by the matrix-assisted technique. We have recently demonstrated that matrix-assisted ultraviolet laser desorption can be successfully used with FTMS for the ionization of small peptides. The objective of this report is to summarize the application and current limitations of matrix-assisted laser desorption FTMS for the characterization of peptides and oligonucleotides at the isomeric level. 4 refs., 3 figs., 2 tabs.
Laser Desorption Ionization Mass Spectrometry Based on Nanophotonic Structure: From Material Design to Mechanistic Understanding
Author: Moon-Ju KimPublisher: Springer Nature
ISBN: 981996878X
Category : Science
Languages : en
Pages : 107
Book Description
This book presents innovative laser desorption ionization (LDI)-active nanophotonic structures for addressing the challenges that matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is currently facing and for enhancing LDI efficiency. It presents a variety of cutting-edge nanophotonic structures to satisfy the mass-analytical needs of sensitivity, reproducibility, and quantification. As opposed to the commercialized, conventional organic matrix used in MALDI-MS, these nanostructures are validated to be highly effective in detecting small metabolites and drugs, highlighting their considerable potential in the mass spectrometry field. It also systematically elucidates fundamental LDI processes in terms of the photo-thermal, electronic, and structural characteristics of nanophotonic structures, offering mechanistic knowledge of LDI-MS. Even though LDI-MS performance is heavily influenced by a number of nanostructure parameters, relatively little is known about the LDI processes associated with those characteristics. An in-depth understanding of nanostructure characteristics and LDI mechanisms thus paves the way for more effective, rational design and development of nanostructures with improved LDI capabilities. Further, with a focus on multiple cascades in nanostructure functions in response to laser pulse stimuli, this book provides detailed, step-by-step procedures to design and construct a nanophotonic, LDI-active platform, which may serve as a roadmap for graduate students in the field of mass spectrometry. Readers, including graduate students, researchers, and experts working in the related areas of mass spectrometry, nanophotonics, and material science and material engineering, will find a wealth of useful information in this book.