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Author: Udo J. Birk Publisher: John Wiley & Sons ISBN: 3527341331 Category : Science Languages : en Pages : 405
Book Description
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Author: Udo J. Birk Publisher: John Wiley & Sons ISBN: 3527341331 Category : Science Languages : en Pages : 405
Book Description
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Author: Publisher: ISBN: Category : Languages : en Pages : 72
Book Description
The oldest and most respected martial arts title in the industry, this popular monthly magazine addresses the needs of martial artists of all levels by providing them with information about every style of self-defense in the world - including techniques and strategies. In addition, Black Belt produces and markets over 75 martial arts-oriented books and videos including many about the works of Bruce Lee, the best-known marital arts figure in the world.
Author: Publisher: ISBN: Category : Languages : en Pages : 176
Book Description
Popular Science gives our readers the information and tools to improve their technology and their world. The core belief that Popular Science and our readers share: The future is going to be better, and science and technology are the driving forces that will help make it better.
Author: Subhasis Chaudhuri Publisher: Springer Science & Business Media ISBN: 0792374711 Category : Computers Languages : en Pages : 287
Book Description
Super-Resolution Imaging serves as an essential reference for both academicians and practicing engineers. It can be used both as a text for advanced courses in imaging and as a desk reference for those working in multimedia, electrical engineering, computer science, and mathematics. The first book to cover the new research area of super-resolution imaging, this text includes work on the following groundbreaking topics: Image zooming based on wavelets and generalized interpolation; Super-resolution from sub-pixel shifts; Use of blur as a cue; Use of warping in super-resolution; Resolution enhancement using multiple apertures; Super-resolution from motion data; Super-resolution from compressed video; Limits in super-resolution imaging. Written by the leading experts in the field, Super-Resolution Imaging presents a comprehensive analysis of current technology, along with new research findings and directions for future work.
Author: Robert K. Brown Publisher: Simon and Schuster ISBN: 1628735414 Category : History Languages : en Pages : 256
Book Description
Becoming an elite sniper involves more than learning how to shoot. Snipers need to know how to judge terrain, wind, and sometimes even the curve of the Earth. They train their eyes to spot enemy movement in the distance and to never hesitate. A sniper is more that a finger behind a trigger; he is a scout, a scientist, a strategist, and the support group for a platoon. Soldier of Fortune Magazine Guide to Super Snipers brings you into the world of some of the most accomplished snipers. From Finland to Iraq, Korea to Somalia, Soldier of Fortune Magazine provides stories from the eyes of the snipers who made the kills. These trained marksmen describe their method of taking the perfect shot, all the way down to the type of chewing tobacco they prefer. Some of the super snipers in this collection include: Major Charles Greene Sergeant Dan Mills Gunnery Sergeant Jack Coughlin Chris Kyle Robert K. Brown And many more! Every sniper—just like every situation that calls for a sniper—is unique, and Soldier of Fortune Magazine Guide to Super Snipers will show you every step of the way, as you find the target, narrow the scope, and take the shot.
Author: Tamas D. Gedeon Publisher: Springer ISBN: 3540245812 Category : Computers Languages : en Pages : 1095
Book Description
Consider the problem of a robot (algorithm, learning mechanism) moving along the real line attempting to locate a particular point ? . To assist the me- anism, we assume that it can communicate with an Environment (“Oracle”) which guides it with information regarding the direction in which it should go. If the Environment is deterministic the problem is the “Deterministic Point - cation Problem” which has been studied rather thoroughly [1]. In its pioneering version [1] the problem was presented in the setting that the Environment could charge the robot a cost which was proportional to the distance it was from the point sought for. The question of having multiple communicating robots locate a point on the line has also been studied [1, 2]. In the stochastic version of this problem, we consider the scenario when the learning mechanism attempts to locate a point in an interval with stochastic (i. e. , possibly erroneous) instead of deterministic responses from the environment. Thus when it should really be moving to the “right” it may be advised to move to the “left” and vice versa. Apart from the problem being of importance in its own right, the stoch- tic pointlocationproblemalsohas potentialapplications insolvingoptimization problems. Inmanyoptimizationsolutions–forexampleinimageprocessing,p- tern recognition and neural computing [5, 9, 11, 12, 14, 16, 19], the algorithm worksits wayfromits currentsolutionto the optimalsolutionbasedoninfor- tion that it currentlyhas. A crucialquestionis oneof determining the parameter whichtheoptimizationalgorithmshoulduse.
Author: Udo J. Birk
Author: Udo J. Birk
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Author: Lester Gray French
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Author: Subhasis Chaudhuri
Author: Robert K. Brown
Author: Tamas D. Gedeon