Studies of Membrane Structure by Freeze-etching. Progress Report, 1 July 1975--30 June 1976. [Erythrocyte Membranes]. PDF Download
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Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The structure and organization of biological membranes were studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts have been probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and electron microscope autoradiographic techniques has been developed and tested and a new method of rotary shadow freeze-etching has been evaluated. The role of lipid-lipid and protein-protein interactions in controlling the mobility of cell surface macromolecules has been studied using freeze-fracture, protein extraction and SDS-polyacrylamide gel techniques.
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The structure and organization of biological membranes were studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts have been probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and electron microscope autoradiographic techniques has been developed and tested and a new method of rotary shadow freeze-etching has been evaluated. The role of lipid-lipid and protein-protein interactions in controlling the mobility of cell surface macromolecules has been studied using freeze-fracture, protein extraction and SDS-polyacrylamide gel techniques.
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The structure and organization of biological membranes has been studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts have been probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and biochemical techniques using fluorescent resonance energy transfer has been developed and tested and ultra low temperature freeze-etching has been evaluated as part of a program to develop covalent labelling techniques that will identify specific transmembrane proteins in freeze-fractured replicas.
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The structure and organization of biological membranes is being studied using a variety of physical and biochemical techniques together with electron microscopy and freeze-etching. These studies are providing knowledge relating the architecture of cellular membranes to their role in modulating cell behavior in response to environmental perturbants and in regulating the transport of molecules into and out of cells. As model systems, human erythrocyte membranes and their ghosts are being probed to determine the distribution and asymmetry of component lipids and proteins. For this purpose, a novel combination of freeze-fracture and biochemical techniques using fluorescent resonance energy transfer has been developed and tested and ultra low temperature freeze-etching has been evaluated as part of a program to develop covalent labelling techniques that will identify specific transmembrane proteins in freeze-fractured replicas.
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
The researchers devised a rapid procedure for measuring covalent bond breakage in a simple model system to determine the extent to which glycophorin was cleaved when the frozen vesicles were fractured. Evidence showed that no covalent bonds are broken during the fracture procedure. Membrane splitting was shown to be quantified under special circumstances when erythrocyte membranes are appressed to a polylysine-coated surface. Results show that freeze fracture at temperatures below that of liquid nitrogen results in the appearance of considerable detail on E faces of purple membranes. Detail seems to improve as the temperature is lowered, suggesting that the smooth E faces in conventional freeze fractures may be a result of plastic deformation and flow of membrane components. Results show that ultra-low temperature freeze-fracturing can reduce plastic distortion and, particularly when used in conjunction with rotary replication, should improve our ability to discern small labels attached to transmembrane proteins. (PCS).
Author: United States. Energy Research and Development Administration. Technical Information Center Publisher: ISBN: Category : Force and energy Languages : en Pages : 1680
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Author: United States. Energy Research and Development Administration
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Author: United States. Energy Research and Development Administration. Technical Information Center
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Author: United States. Energy Research and Development Administration
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