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Author: Henrik J. Ditzel Publisher: ISBN: 9781071612989 Category : Drug development Languages : en Pages : 469
Book Description
This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field. This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field.
Author: Henrik J. Ditzel Publisher: ISBN: 9781071612989 Category : Drug development Languages : en Pages : 469
Book Description
This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field. This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field.
Author: Debra J. Taxman Publisher: Humana Press ISBN: 9781627031202 Category : Science Languages : en Pages : 392
Book Description
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular BiologyTM series, this work contains the kind of detailed description and implementation advice that guarantees successful results. Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.
Author: Bin Wang Publisher: CRC Press ISBN: 9814411647 Category : Medical Languages : en Pages : 468
Book Description
In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.
Author: Yuen-Chi Mui Publisher: Open Dissertation Press ISBN: 9781374719156 Category : Computers Languages : en Pages : 76
Book Description
This dissertation, "Comparison and Improvement of SiRNA Design Tools" by Yuen-chi, Mui, 梅宛芝, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of Thesis Entitled \Comparison and Improvement of siRNA Design Tools" Submitted by Mui Yuen Chi For the Degree of Master of Philosophy at The University of Hong Kong in August 2004 Short interfering ribonucleic acids (siRNAs) can be used to prevent a gene from performing its functions. They therefore have many potential applications in therapy. The siRNA design problem is equivalent to nding substrings from a string, known as messenger ribonucleic acid (mRNA), ranging in length from hundreds to tens of thousands of characters. However, not every substring can form an eective siRNA. In fact, the criteria for designing eective siRNAs re- main unclear. Expensive and time-consuming experiments are required to verify whether a given siRNA is eective. Based on the Max Planck Institute (MPI) siRNA user guide, a number of siRNA design tools have been developed in the past three years. The set of candidates reported by these tools is usually large and often contains some inef- fective siRNAs. This study has therefore investigated the possibility of ltering ineective siRNAs. It makes three main contributions. It proposes, for the rst time, a scheme to compare the performance of the tools based on real data in the literature. It also attempts to improve the existing tools by an algorithm that can lter ineective siRNAs. The ltering algorithm is based on some new observations on the secondary structure of the mRNA, which have been veried by articial intelligence techniques (decision trees and support vector machines). The algorithm successfully lters most of the published ineective siRNAs and retains most of the published eective siRNAs reported by the existing tools. Finally, it presents a new implementation of siRNA design web-based software. This software is a platform for using existing tools at one time. The software also enhances the output from the existing tools by applying the ltering algorithm. DOI: 10.5353/th_b3072201 Subjects: Small interfering RNA - Data processing Computer algorithms
Author: Dr. Tim Doran Publisher: CABI ISBN: 1845934105 Category : Science Languages : en Pages : 269
Book Description
This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.
Author: Mouldy Sioud Publisher: Humana Press ISBN: 9781607616641 Category : Science Languages : en Pages : 527
Book Description
Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular BiologyTM series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes. Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.
Author: R. K. Gaur Publisher: CRC Press ISBN: 1439873259 Category : Science Languages : en Pages : 380
Book Description
RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA
Author: Gail Carolyn Sirna Publisher: ISBN: Category : Art Languages : en Pages : 200
Book Description
"Women of all times, places, and stations in life have taken needle in hand, either for the most utilitarian of purposes or for artistic reasons. Thsi charming collection of beautiful paintings celebrates the centuries-old iconography of women engaged in needle work and the related arts of knitting, lacemaking, and weaving. Artists have long sought to capture the needlewoman's quiet concertration and artistic endeavor, and many of the loveliest paintings on this theme, from the sixteenth century onward, are presented in this book"--Page 2 of cover.
Author: Loutfy H. Madkour Publisher: CRC Press ISBN: 1000532240 Category : Science Languages : en Pages : 860
Book Description
This book presents an overview of the current status of translating the RNAi cancer therapeutics in the clinic, a brief description of the biological barriers in drug delivery, and the roles of imaging in aspects of administration route, systemic circulation, and cellular barriers for the clinical translation of RNAi cancer therapeutics, and with partial content for discussing the safety concerns. It then focuses on imaging-guided delivery of RNAi therapeutics in preclinical development, including the basic principles of different imaging modalities, and their advantages and limitations for biological imaging. With growing number of RNAi therapeutics entering the clinic, various imaging methods will play an important role in facilitating the translation of RNAi cancer therapeutics from bench to bedside. RNAi technique has become a powerful tool for basic research to selectively knock down gene expression in vitro and in vivo. Our scientific and industrial communities have started to develop RNAi therapeutics as the next class of drugs for treating a variety of genetic disorders, such as cancer and other diseases that are particularly hard to address with current treatment strategies. Key Features Provides insight into the current advances and hurdles of RNAi therapeutics. Accelerates RNAi, miRNAs, and siRNA drug development for cancer therapy from bench to bedside. Addresses various modifications and novel delivery strategies for miRNAs, piRNAs and siRNA delivery in anticancer therapeutics. Explores the need for the interaction of hematologists,cell biologists, immunologists, and material scientists in the development of novel cancer therapies. Describes the current status of clinical trials related to miRNA and siRNA-based cancer therapy Presents remaining issues that need to be overcome to establish successful therapies.
Author: Henrik J. Ditzel
Author: Henrik J. Ditzel
Author: Debra J. Taxman
Author: Bin Wang
Author: Yuen-Chi Mui
Author: Dr. Tim Doran
Author: Mouldy Sioud
Author: R. K. Gaur
Author: Gail Carolyn Sirna
Author: Phillip Angart
Author: Loutfy H. Madkour