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Single-Molecule Science

Single-Molecule Science PDF Author: Krishnarao Appasani
Publisher: Cambridge University Press
ISBN: 1108423361
Category : Medical
Languages : en
Pages : 171

Book Description
A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.

Single-Molecule Science

Single-Molecule Science PDF Author: Krishnarao Appasani
Publisher: Cambridge University Press
ISBN: 1108423361
Category : Medical
Languages : en
Pages : 171

Book Description
A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.

Biology at the Single Molecule Level

Biology at the Single Molecule Level PDF Author: S.H. Leuba
Publisher: Elsevier
ISBN: 008056061X
Category : Science
Languages : en
Pages : 263

Book Description
This is the first book solely devoted to single-molecule biochemistry and molecular biology. Authors were selected on the basis of their contribution to this new and exciting field, and were asked to focus more on the biological problems that can be approached using single-molecule techniques rather than on the techniques per se. It is thought that such techniques will eventually dominate the physical characterization of biologically important macromolecules.

Single Molecule Biology

Single Molecule Biology PDF Author: Alexander E. Knight
Publisher: Academic Press
ISBN: 008092123X
Category : Science
Languages : en
Pages : 369

Book Description
Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. - Organized by biological system or molecule - Each chapter discusses insights into mechanism of action, structure, and function - Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc - Includes an introduction to key principles and an historical overview - Discusses applications in molecular diagnostics and drug discovery - Provides an expert's perspective on future development

An Introduction to Single Molecule Biophysics

An Introduction to Single Molecule Biophysics PDF Author: Yuri L. Lyubchenko
Publisher: CRC Press
ISBN: 1351333127
Category : Science
Languages : en
Pages : 240

Book Description
This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.

Single Molecule Spectroscopy in Chemistry, Physics and Biology

Single Molecule Spectroscopy in Chemistry, Physics and Biology PDF Author: Astrid Graslund
Publisher: Springer Science & Business Media
ISBN: 3642025986
Category : Molecular spectroscopy
Languages : en
Pages : 570

Book Description
Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

Handbook of Single-Molecule Biophysics

Handbook of Single-Molecule Biophysics PDF Author: Peter Hinterdorfer
Publisher: Springer Science & Business Media
ISBN: 0387764976
Category : Science
Languages : en
Pages : 634

Book Description
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Single-Molecule Cellular Biophysics

Single-Molecule Cellular Biophysics PDF Author: Mark C. Leake
Publisher: Cambridge University Press
ISBN: 1107005833
Category : Science
Languages : en
Pages : 291

Book Description
Indispensable textbook for undergraduate students in the physical and life sciences, unravelling the inner workings of the cell.

Single Molecule Analysis

Single Molecule Analysis PDF Author: Erwin J. G. Peterman
Publisher: Humana Press
ISBN: 9781617792816
Category : Science
Languages : en
Pages : 317

Book Description
Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

Single-molecule Techniques

Single-molecule Techniques PDF Author: Paul R. Selvin
Publisher: CSHL Press
ISBN: 087969775X
Category : Science
Languages : en
Pages : 511

Book Description
Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.

Single Molecule Science

Single Molecule Science PDF Author: Dmitrii E. Makarov
Publisher: CRC Press
ISBN: 1466559527
Category : Science
Languages : en
Pages : 212

Book Description
The observation and manipulation of individual molecules is one of the most exciting developments in modern molecular science. Single Molecule Science: Physical Principles and Models provides an introduction to the mathematical tools and physical theories needed to understand, explain, and model single-molecule observations. This book explains the