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Author: Zhihang Yuan Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
The enhanced biological phosphorus removal (EBPR) has been widely applied in treating domestic wastewater, while the performance on high-strength P wastewater is less investigated and the feasibility of coupling with short-cut nitrogen removal process remains unknown. This study first achieved the simultaneous high-efficient P removal and stable nitrite accumulation in one sequencing batch reactor for treating the synthetic digested manure wastewater. The average effluent P could be down to 0.8 ± 1.0 mg P/L and the P removal efficiency was 99.5 ± 0.8%. Candidatus Accumulibacter was the dominant polyphosphate accumulating organism with the relative abundance of 14.2-33.1%, while the presence of glycogen accumulating organisms (Defluviicoccus and Candidatus Competibacter) with relatively higher abundance (15.6-40.3%) did not deteriorate the EBPR performance. Moreover, nitrite accumulation happened in the system with the effluent nitrite up to 20.4 ± 6.4 mg N/L and the nitrite accumulation ratio was nearly 100% for 140 days (420 cycles). Nitrosomonas was the dominant ammonia-oxidizing bacteria with relative abundance of 0.3-2.4% while nitrite-oxidizing bacteria were almost undetected (
Author: Zhihang Yuan Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
The enhanced biological phosphorus removal (EBPR) has been widely applied in treating domestic wastewater, while the performance on high-strength P wastewater is less investigated and the feasibility of coupling with short-cut nitrogen removal process remains unknown. This study first achieved the simultaneous high-efficient P removal and stable nitrite accumulation in one sequencing batch reactor for treating the synthetic digested manure wastewater. The average effluent P could be down to 0.8 ± 1.0 mg P/L and the P removal efficiency was 99.5 ± 0.8%. Candidatus Accumulibacter was the dominant polyphosphate accumulating organism with the relative abundance of 14.2-33.1%, while the presence of glycogen accumulating organisms (Defluviicoccus and Candidatus Competibacter) with relatively higher abundance (15.6-40.3%) did not deteriorate the EBPR performance. Moreover, nitrite accumulation happened in the system with the effluent nitrite up to 20.4 ± 6.4 mg N/L and the nitrite accumulation ratio was nearly 100% for 140 days (420 cycles). Nitrosomonas was the dominant ammonia-oxidizing bacteria with relative abundance of 0.3-2.4% while nitrite-oxidizing bacteria were almost undetected (
Author: S. Bathe Publisher: IWA Publishing ISBN: 9781843395096 Category : Science Languages : en Pages : 186
Book Description
Aerobic Granular Sludge has recently received growing attention by researchers and technology developers, worldwide. Laboratory studies and preliminary field tests led to the conclusion that granular activated sludge can be readily established and profitably used in activated sludge plants, provided 'correct' process conditions are chosen. But what makes process conditions 'correct'? And what makes granules different from activated sludge flocs? Answers to these question are offered in Aerobic Granular Sludge. Major topics covered in this book include: Reasons and mechanism of aerobic granule formation Structure of the microbial population of aerobic granules Role, composition and physical properties of EPS Diffuse limitation and microbial activity within granules Physio-chemical characteristics Operation and application of granule reactors Scale-up aspects of granular sludge reactors, and case studies Aerobic Granular Sludge provides up-to-date information about a rapidly emerging new technology of biological treatment.
Author: Rogelio Ernesto Zuniga Montanez Publisher: ISBN: Category : Languages : en Pages :
Book Description
Municipal wastewater contains a number of constituents that can have detrimental effects if discharged to receiving water bodies. Phosphorus (P) is of specific interest as a limiting nutrient in aquatic ecosystems that can cause eutrophication. In enhanced biological phosphorus removal (EBPR), polyphosphate accumulating organisms (PAOs) store excess P intracellularly. To achieve this accumulation, the organisms are exposed consecutively to anaerobic and either aerobic or anoxic conditions. During the anaerobic phase, PAOs consume and store organic carbon with P release, followed by the aerobic/anoxic phase during which the stored carbon is oxidized and P is taken up and stored as polyphosphate. PAOs are not the only bacteria that can thrive under these cyclic conditions and they face competition from glycogen accumulating organisms (GAOs). The latter have a similar metabolism but do not accumulate P. Most research to date has focused on the use of certain volatile fatty acids (VFAs) as carbon sources and on process conditions at temperatures common in temperate climates. Much remains unknown about the potential of EBPR in tropical regions and the suitability of other carbon substrates to drive the accumulation of phosphate. The purpose of this dissertation is to contribute to the understanding of EBPR at high temperatures and with unconventional carbon sources. Three different studies were designed and conducted with the following aims: (a) to evaluate the long-term EBPR stability and key microbial community in a wastewater treatment plant (WWTP) designed to achieve P removal in Singapore, (b) to study the process efficiency, biochemical transformations and organisms involved in a laboratory-scale EBPR reactor fed by alternating the substrates acetate and glutamate, and (c) to assess the potential of using unconventional carbon sources for EBPR by testing glutamate and glucose as alternating substrates at the laboratory-scale. The research included experiments at the full- and laboratory-scale, all at a mean temperature of 30 °C. Sustained observations in all three studies served to uncover the biochemical and microbial community dynamics. In the full-scale study, I conducted a yearlong evaluation of the EBPR activity at a WWTP that had been retrofitted to facilitate EBPR in Singapore. A mean P removal efficiency of 90 % was observed throughout the sampling period, similar to temperate climate installations and contrary to earlier reports that EBPR was not feasible at high temperatures. The main PAOs present in the reactor were Tetrasphaera, Candidatus Accumulibacter (Accumulibacter) and Dechloromonas, with mean relative abundances of 1.53, 0.43 and 0.69 %, respectively. The PAO community underwent changes during the surveyed period, with a marked transition from a Tetrasphaera-dominated community to a more even one. The link between PAOs and the P released in the anaerobic compartment was supported by a statistically significant correlation between the relative abundance of these organisms and the measured P concentrations. GAOs and PAOs coexisted without compromising the EBPR activity. In one of the laboratory-scale studies, glutamate and acetate were alternated as the carbon source for a reactor operated at 30 °C. Complete and stable P removal was achieved with a predominantly glutamate-containing feed, after modifying operating parameters commonly used in VFA-fed systems to a COD/P ratio of 40:1 mg COD/mg P and a cycle duration of 8 h. Long-term EBPR with a feed dominated by glutamate in a laboratory-scale reactor has not been previously reported. The P and carbon cycling patterns were different for glutamate and acetate. Reduced P release and polyhydroxyalkanoate (PHA) accumulation happened when glutamate was fed, but not with acetate, where glutamate appeared to be stored as an unidentified non-PHA compound or as different compounds. The PAO Accumulibacter and the GAO Candidatus Competibacter (Competibacter) remained the only known EBPR bacteria during the period of good EBPR performance, at similar relative abundances. A canonical correlation analysis revealed that the relative abundance of some non-PAO organisms correlated more strongly with variables that denoted good EBPR activity than did the abundance of any of the known PAOs. In the last study, a laboratory-scale sequencing batch reactor was used to test the EBPR potential of glutamate and glucose as alternating carbon sources in a high temperature process. The recommended influent COD/P ratio and batch duration for VFA-fed systems were unsuccessful. After modifications, COD/P ratios of 20:1 and 40:1 mg COD/ mg P resulted in complete P removal, but only in the short term. The EBPR stoichiometry with these two carbon substrates differed from that of VFA-fed systems. For both, lower P and PHA cycling was observed, and intracellular carbon storage compounds that were not PHA appeared to contribute to P cycling, as shown from carbon balances. A very diverse EBPR community was present in the reactor, including Accumulibacter, Tetrasphaera and Dechloromonas PAOs, and Competibacter, Defluviicoccus, Micropruina and Kineosphaera GAOs. Most of these organisms have not been reported before in laboratory-scale EBPR reactors operated at high temperatures. The work presented in this dissertation expands the understanding of EBPR by showing that the process is possible and stable in full-scale treatment plants at high temperature, with removal efficiencies similar to those observed in temperate climates. In addition, it was shown that unconventional carbon sources, specifically, glutamate and glucose, do participate in EBPR and that complete and stable phosphorus removal can be achieved with glutamate as dominant substrate at high temperature. A core PAO and GAO community was present in the three systems, where the interactions among members were more complex than previously considered, including competition, coexistence and succession events. The results obtained from this work enhance our fundamental knowledge of EBPR as an industrial process, as well as the metabolic diversity, niches and dynamics of PAOs and GAOs. The study outcomes can inform design and operational strategies at full-scale treatment plants. Lastly, the consideration of both high temperatures and unconventional carbon sources for EBPR is expected to aid in the development of more efficient treatment processes.
Author: Maryam Reza Publisher: ISBN: Category : Denitrification Languages : en Pages : 188
Book Description
The objective of this PhD thesis was to study, develop and analyze an effective bioreactor and biological process to simultaneously remove nitrogen and phosphorus compounds from wastewater with minimum requirements for energy and footprint. A novel biological nutrient removal process was developed in a vertically configured pilot-scale bioreactor. The bioreactor set-up and its biological process were undertaken in the Water Technologies Laboratory at Ryerson University from November 2012 to December 2013. The bioreactor consisted of three consecutive vertical stages including Anoxic 1, Anoxic 2 and Aerobic stages. The reactor was aligned with an Anaerobic Lateral Unit (ALU) which provided a strict anaerobic condition to cultivate and promote the growth of phosphorus accumulating organisms (PAOs). The unique features of the bioreactor are the foundations for development of a simultaneous nitrification-denitrification-biological phosphorus removal process (SNDP). This thesis reveals that SNDP is the main pathway for the removal of ammonia, nitrate, nitrite and phosphorus. The SNDP process shows high level of nitrogen and phosphorus compounds removal with over 95% phosphorous and nitrogen removal efficiencies during one (1) year of laboratory operations. The co-existence of microorganisms in the SNDP process was highly influenced by the actions of all three redox conditions, 1) anaerobic, 2) anoxic, and 3) aerobic zones. The redox variations were influenced by the bioreactor configuration, HRTs, SRTs and nutrient concentrations. The biomass samples from the bioreactor were studied rigorously using advanced molecular biology techniques such as genomic sequencing. Microbial structure, diversity and interactions in the SNDP were studied in details. The results obtained in this work proved the presence of a new genus of microorganism known as Saprospiracae which occupied more than 67% of the biomass in the Anoxic stages and 48% of the biomass in the Aerobic stage of the bioreactor. The second largest group belonged to the genus of Zoogloea with over 11% and 36% in the Anoxic and Aerobic stages respectively. The vertical continuous flow bioreactor developed and operated in this research created a unique habitat for the growth of these microorganisms. To optimize the SNDP process, a series of experiments were performed on the bioreactor by varying three important parameters including: 1) Dissolved Oxygen (DO) concentration in the Aerobic stage ranging from 0-0.5 to 5.5-6 mg/L; 2) COD concentration in the Anaerobic stage ranging from 1000 to 1400 mg/L and, 3) NH3-N concentration in the inlet ranging from 49 to 120 mg/L. The experimental results showed that DO in the range of 2.5-3 mg/L were the optimum concentration for the SNDP process. The COD concentrations fed to the ALU could be lowered to 1000mg/L at SRT of approximately 50 days. Moreover, the intracellular PHAs in the biomass was found to be a great asset for the SNDP process as COD addition to the ALU could be lowered from 1400 mg/L to 1000 mg/L without having any negative impact on the process. The inlet NH3-N concentration of 49 mg/L was found to be the optimum level for the SNDP process. Biological phosphorus uptake was negatively affected when NH3-N concentration in the inlet was increased from 49 mg/L to 120 mg/L. The negative effects of high NH3-N were likely caused by increase in NO2- concentration and accumulation in the bioreactor which inhibited the activities of the PAOs. Both the SNDP process developed in this research and the vertical continuous flow bioreactor are innovations in the area of water/wastewater treatment. Indeed, the microbial distributions in anoxic and aerobic environment have not been found in any laboratory scale nor large scale BNR plant to date.
Author: Korneel Rabaey Publisher: IWA Publishing ISBN: 184339233X Category : Science Languages : en Pages : 525
Book Description
In the context of wastewater treatment, Bioelectrochemical Systems (BESs) have gained considerable interest in the past few years, and several BES processes are on the brink of application to this area. This book, written by a large number of world experts in the different sub-topics, describes the different aspects and processes relevant to their development. Bioelectrochemical Systems (BESs) use micro-organisms to catalyze an oxidation and/or reduction reaction at an anodic and cathodic electrode respectively. Briefly, at an anode oxidation of organic and inorganic electron donors can occur. Prime examples of such electron donors are waste organics and sulfides. At the cathode, an electron acceptor such as oxygen or nitrate can be reduced. The anode and the cathode are connected through an electrical circuit. If electrical power is harvested from this circuit, the system is called a Microbial Fuel Cell; if electrical power is invested, the system is called a Microbial Electrolysis Cell. The overall framework of bio-energy and bio-fuels is discussed. A number of chapters discuss the basics – microbiology, microbial ecology, electrochemistry, technology and materials development. The book continues by highlighting the plurality of processes based on BES technology already in existence, going from wastewater based reactors to sediment based bio-batteries. The integration of BESs into existing water or process lines is discussed. Finally, an outlook is provided of how BES will fit within the emerging biorefinery area.
Author: Edward F. DeLong Publisher: Springer ISBN: 9783642301421 Category : Science Languages : en Pages : 662
Book Description
The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea
Author: Zhihang Yuan
Author: Zhihang Yuan
Author: Rafael Perez-Feito
Author: S. Bathe
Author: Rogelio Ernesto Zuniga Montanez
Author: Maryam Reza
Author: 
Author: Korneel Rabaey
Author: Edward F. DeLong
Author: Devang D. Patel
Author: Shuh-Ren Jing