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Author: David Williams Publisher: ISBN: 9781999618933 Category : Languages : en Pages : 242
Book Description
This book will serve you well if: You're new to traditional stained glass painting and are uncertain which brushes, paints and tools to buy - this book will tell you what you want to know. The accompanying 12 free videos will also introduce you to the key techniques. You've painted stained glass for a while but still worry "Is it my lack of skill which is the problem, or is it my brushes, paints and tools which stop me achieving the results I want?" - this book will guide you to the answer. And the 23 free designs will give you an excellent resource with which to practise. You're a teacher who wants your students to experience the joy of mastering this wonderful craft - this book will show you the smallest, most effective set of brushes, paints and tools to place before them so they can triumph. From designers and painters Williams & Byrne, and featuring entries from the journal of the forgotten Victorian craftsman Nathaniel Somers, this book will demonstrate how few possessions you really need to paint stained glass. This is Book 1 of The Glass Painter's Method.
Author: David Williams Publisher: ISBN: 9781999618933 Category : Languages : en Pages : 242
Book Description
This book will serve you well if: You're new to traditional stained glass painting and are uncertain which brushes, paints and tools to buy - this book will tell you what you want to know. The accompanying 12 free videos will also introduce you to the key techniques. You've painted stained glass for a while but still worry "Is it my lack of skill which is the problem, or is it my brushes, paints and tools which stop me achieving the results I want?" - this book will guide you to the answer. And the 23 free designs will give you an excellent resource with which to practise. You're a teacher who wants your students to experience the joy of mastering this wonderful craft - this book will show you the smallest, most effective set of brushes, paints and tools to place before them so they can triumph. From designers and painters Williams & Byrne, and featuring entries from the journal of the forgotten Victorian craftsman Nathaniel Somers, this book will demonstrate how few possessions you really need to paint stained glass. This is Book 1 of The Glass Painter's Method.
Author: Gerhard W. Hacker Publisher: CRC Press ISBN: 9780849313929 Category : Medical Languages : en Pages : 264
Book Description
Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know about the various staining methods, their useful applications, and the advantages and drawbacks of each process. Gold and Silver Staining: Techniques in Molecular Morphology provides a timely description of approaches, methods, protocols, and applications. The contributors cover the latest developments and a wide range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. Some of the authors are "living legends" in the field and bring their expertise and experience to this fine collaboration. Written in one consistent style, each chapter includes a concise, but comprehensive introduction, step-by-step protocols with technical hints, and a discussion of results and critical steps. What differentiates this book from all others like it, is the status of the editors, who have worked on this technique from its inception and have produced innumerable publications on the topic. The other distinguishing feature is that all of the contributors are amongst the absolute foremost leaders from the United States and Europe. Gold and Silver Staining: Techniques in Molecular Morphology presents a complete overview and detailed descriptions of this technique that allows the visualization of molecules that have never been localized before and with hitherto unknown sensitivity. Not only does this book provide an excellent review of this field, but it also serves as a lab manual for those who want to carry out this technique in their laboratory and clinical work. Armed with this information, advances in this powerful field of research will ensue.
Author: Biji T. Kurien Publisher: Humana ISBN: 9781493993819 Category : Science Languages : en Pages : 289
Book Description
This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.
Author: Marilyn S. Arsham Publisher: John Wiley & Sons ISBN: 1119061229 Category : Science Languages : en Pages : 1216
Book Description
Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
Author: Robert E. Farrell Jr. Publisher: Elsevier ISBN: 0080454763 Category : Science Languages : en Pages : 794
Book Description
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Author: Jordi Llorens Publisher: Springer Nature ISBN: 1071616374 Category : Medical Languages : en Pages : 488
Book Description
This volume explores the latest methods that seek to address the vital questions being asked in neurotoxicology research. The chapters in this book cover a variety of available methods from the molecular level to the organism level, and both in vitro and in vivo approaches, including alternative model organisms. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Experimental Neurotoxicology Methods is a valuable resource for both young and experienced researchers who are looking for guidance to implement these methods in their laboratories or for understanding the data generated through these techniques.
Author: Biji T. Kurien Publisher: Humana Press ISBN: 9781617798207 Category : Science Languages : en Pages : 0
Book Description
Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.
Author: John M. Walker Publisher: Springer Science & Business Media ISBN: 0896033384 Category : Electronic books Languages : en Pages : 759
Book Description
-- 145 concise, step-by-step protocols for the analysis of proteins and peptides -- Provides more than three times as many protocols than the competitors' manuals -- in the incomparable Methods in Molecular Biology "TM" style -- at an affordable price -- Combound in a large 7 x l0 inch format for easy benchtop use -- An indispensable reference for industry-based researchers, professors, and their students
Author: Laszlo Zaborszky Publisher: Springer Science & Business Media ISBN: 1475720556 Category : Medical Languages : en Pages : 429
Book Description
This new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.
Author: M. A. Hayat Publisher: CRC Press ISBN: 9780849324499 Category : Science Languages : en Pages : 344
Book Description
This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.
Author: David Williams
Author: David Williams
Author: Gerhard W. Hacker
Author: Biji T. Kurien
Author: Marilyn S. Arsham
Author: Robert E. Farrell Jr.
Author: Jordi Llorens
Author: Biji T. Kurien
Author: John M. Walker
Author: Laszlo Zaborszky
Author: M. A. Hayat