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Author: Publisher: ISBN: Category : Languages : en Pages : 54
Book Description
A recent advance in immunogold technology has been the use of molecular gold instead of colloidal gold. A number of advantages are realized by this approach, such as stable covalent, site-specific attachment, small probe size and absence of aggregates for improved penetration. Silver enhancement has led to improved and unique results for electron and light microscopy, as well as their use with blots and gels. Most previous work with immunogold silver staining has been done with colloidal gold particles. More recently, large gold compounds (''clusters'') having a definite number of gold atoms and defined organic shell, have been used, frequently with improved results. These gold dusters, large compared to simple compounds, are, however, at the small end of the colloidal gold scale in size; undecagold is 0.8 nm and Nanogold is 1.4 nm. They may be used in practically all applications where colloidal gold is used (Light and electron microscopy, dot blots, etc.) and in some unique applications, where at least the larger colloidal golds don't work, such as running gold labeled proteins on gels (which are later detected by silver enhancement). The main differences between gold clusters and colloidal golds are the small size of the dusters and their covalent attachment to antibodies or other molecules.
Author: Publisher: ISBN: Category : Languages : en Pages : 54
Book Description
A recent advance in immunogold technology has been the use of molecular gold instead of colloidal gold. A number of advantages are realized by this approach, such as stable covalent, site-specific attachment, small probe size and absence of aggregates for improved penetration. Silver enhancement has led to improved and unique results for electron and light microscopy, as well as their use with blots and gels. Most previous work with immunogold silver staining has been done with colloidal gold particles. More recently, large gold compounds (''clusters'') having a definite number of gold atoms and defined organic shell, have been used, frequently with improved results. These gold dusters, large compared to simple compounds, are, however, at the small end of the colloidal gold scale in size; undecagold is 0.8 nm and Nanogold is 1.4 nm. They may be used in practically all applications where colloidal gold is used (Light and electron microscopy, dot blots, etc.) and in some unique applications, where at least the larger colloidal golds don't work, such as running gold labeled proteins on gels (which are later detected by silver enhancement). The main differences between gold clusters and colloidal golds are the small size of the dusters and their covalent attachment to antibodies or other molecules.
Author: M. A. Hayat Publisher: CRC Press ISBN: 9780849324499 Category : Science Languages : en Pages : 344
Book Description
This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.
Author: Publisher: ISBN: Category : Languages : en Pages : 19
Book Description
For many applications, silver salt-based autometallography (often also called silver enhancement or silver development) is required to visualize colloidal gold (1-5 nm in diameter) or the small 1.4 nm Nanogold{reg_sign} particles (Nanoprobes, Yaphank, NY, USA). Although even Nanogold may be seen directly by scanning-transmission electron microscopy (STEM), by transmission EM (TEM; in thin sections without stain or ice-embedded cryo-EM samples), energy filtered TEM, and scanning EM (SEM), silver enhancement makes viewing in the EM more facile since the particles are enlarged to approximately 10 to 20 nm, convenient for most specimens. Autometallographic (AMG) enhancement is required in order to visualize smaller gold particles such as Nanogold for light microscopy (LM) or in blots or gels. This chapter includes the following protocols: Protocol for HQ silver enhancement of Nanogold; Protocols for use of silver-enhanced Nanogold with osmium tetroxide--(A) Procedure using reduced concentration of OsO4; (B) Procedures for gold toning; Protocol for HQ silver enhancement of Nanogold in pre-embedding immunocytochemistry for cell cultures; Protocol for gold enhancement of Nanogold for EM; Protocol for gold enhancement of Nanogold for LM; Protocol for staining blots with Nanogold and silver enhancement; and Protocol for staining gels with Nanogold and silver enhancement.
Author: Suban K. Sahoo Publisher: Elsevier ISBN: 0323994555 Category : Technology & Engineering Languages : en Pages : 454
Book Description
Gold and Silver Nanoparticles: Synthesis and Applications provides detailed information on the preparation and utilization of Au- and Ag-based nanoparticles in a range of novel areas. Gold and silver nanoparticles offer a range of interesting properties, including unique size-dependent optoelectronic properties, chemical stability and biocompatibility, ease of synthesis and surface modification, excellent resistance to corrosion, and catalytic properties, hence paving the way to a wide range of cutting-edge applications with continual advances and innovations. Sections introduce gold and silver nanoparticles, fundamental theory, synthesis, and characterization techniques before focusing on requirements and preparation methods. Specific applications areas, such as surface-enhanced Raman spectroscopy (SERS), sensing and biosensing, imaging, drug and gene delivery, disease diagnosis, catalysis, and optoelectronic device fabrication are covered. Finally, synthesis and applications of platinum- and palladium-based nanoparticles are discussed. This is a valuable resource for researchers and advanced students across nanoscience and nanotechnology, chemistry, and materials science, as well as scientists, engineers, and R&D professionals with an interest in noble metal nanomaterials for a range of industrial applications. Explains theory, synthesis, characterization, and properties of Au- and Ag- based nanoparticles Explores a range of novel applications across biomedicine, optoelectronics, and other areas Analyzes the latest developments in the field and considers noble metal nanoparticles beyond gold and silver
Author: Gerhard W. Hacker Publisher: CRC Press ISBN: 1420040235 Category : Medical Languages : en Pages : 265
Book Description
Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a
Author: J. F.POWELL HAINFELD (R. D.FURUYA, F. R.) Publisher: ISBN: Category : Languages : en Pages : 23
Book Description
Gold has been used for immunocytochemistry since 1971 when Faulk and Taylor discovered adsorption of antibodies to colloidal gold. It is an ideal label for electron microscopy (EM) due to its high atomic number, which scatters electrons efficiently, and the fact that preparative methods have been developed to make uniform particles in the appropriate size range of 5 to 30 nm. Use in light microscopy (LM) generally requires silver enhancement (autometallography; AMG) of these small gold particles. Significant advances in this field since that time have included a better understanding of the conditions for best antibody adsorption, more regular gold size production, adsorption of other useful molecules, like protein A, and advances in silver enhancement. Many studies have also been accomplished showing the usefulness of these techniques to cell biology and biomedical research. A further advance in this field was the development of Nanogold{trademark}, a 1.4 nm gold cluster. A significant difference from colloidal gold is that Nanogold is actually a coordination compound containing a gold core covalently linked to surface organic groups. These in turn may be covalently attached to antibodies. This approach to immunolabeling has several advantages compared to colloidal gold such as vastly better penetration into tissues, generally greater sensitivity, and higher density of labeling. Since Nanogold is covalently coupled to antibodies, it may also be directly coupled to almost any protein, peptide, carbohydrate, or molecule of interest, including molecules which do not adsorb to colloidal gold. This increases the range of probes possible, and expands the applications of gold labeling.
Author: Boris Osovetsky Publisher: Springer ISBN: 3319591592 Category : Science Languages : en Pages : 150
Book Description
This book summarizes systematic data on nanogold in geological objects, including mineral-concentrators of nanogold, and the structure and chemical composition of nanogold aggregates. The book also discusses problems that arise during the development of nanogold resources and provides recommendations for prospering new gold deposits with thin-dispersed gold. Electronic microphotos and microprobe analyses support this comprehensive overview of the genesis of nanogold. The book especially focuses on the genesis of nanogold, the processes of nanogold concentration in natural environments, and geological formations containing nanogold.
Author: Catherine Louis Publisher: World Scientific ISBN: 1786341263 Category : Science Languages : en Pages : 681
Book Description
Gold Nanoparticles for Physics, Chemistry and Biology offers an overview of recent research into gold nanoparticles, covering their discovery, usage and contemporary practical applications.This Second Edition begins with a history of over 2000 years of the use of gold nanoparticles, with a review of the specific properties which make gold unique. Updated chapters include gold nanoparticle preparation methods, their plasmon resonance and thermo-optical properties, their catalytic properties and their future technological applications. New chapters have been included, and reveal the growing impact of plasmonics in research, with an introduction to quantum plasmonics, plasmon assisted catalysis and electro-photon conversion. The growing field of nanoparticles for health is also addressed with a study of gold nanoparticles as radiosensibiliser for radiotherapy, and of gold nanoparticle functionalisation. This new edition also considers the relevance of bimetallic nanoparticles for specific applications.World-class scientists provide the most up-to-date findings for an introduction to gold nanoparticles within the related areas of chemistry, biology, material science, optics and physics. It is perfectly suited to advanced level students and researchers looking to enhance their knowledge in the study of gold nanoparticles.
Author: Gary C. Howard Publisher: CRC Press ISBN: 1439869081 Category : Medical Languages : en Pages : 462
Book Description
Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science—both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects of how to make and use antibodies for research along with their commercial and industrial applications. Keeping pace with new developments in this area, all chapters in this new edition have been revised, updated, or expanded. Along with discussions of current applications, new material in the book includes chapters on western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present clear descriptions of basic methods for making and using antibodies and supply detailed descriptions of basic laboratory techniques. Each chapter begins with introductory material, allowing for a better understanding of each concept, and practical examples are included to help readers grasp the real-world scenarios in which antibodies play a part. From the eradication of smallpox to combating cancer, antibodies present an attractive solution to a range of biomedical problems. They are relatively easy to make and use, have great flexibility in applications, and are cost effective for most labs. This volume will assist biomedical researchers and students and pave the way for future discovery of new methods for making and using antibodies for a host of applications.
Author: Gerhard W. Hacker Publisher: CRC Press ISBN: 0203503481 Category : Medical Languages : en Pages : 328
Book Description
Molecular Morphology in Human Tissues: Techniques and Applications presents the most advanced molecular morphological techniques to date. This integrated approach to molecular morphology provides powerful analytical and diagnostic tools at the genome level, making the diagnosis and management of cancer, viral infections, and other diseases more pre
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Author: M. A. Hayat
Author: 
Author: Suban K. Sahoo
Author: Gerhard W. Hacker
Author: J. F.POWELL HAINFELD (R. D.FURUYA, F. R.)
Author: Boris Osovetsky
Author: Catherine Louis
Author: Gary C. Howard
Author: Gerhard W. Hacker